Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4293 and Pf1N1B4_4294 are separated by 431 nucleotides Pf1N1B4_4294 and Pf1N1B4_4295 overlap by 1 nucleotides Pf1N1B4_4295 and Pf1N1B4_4296 are separated by 119 nucleotides
Pf1N1B4_4293 - hypothetical protein, at 4,757,615 to 4,757,863
_4293
Pf1N1B4_4294 - FIG00954439: hypothetical protein, at 4,758,295 to 4,758,744
_4294
Pf1N1B4_4295 - FIG00954434: hypothetical protein, at 4,758,744 to 4,758,947
_4295
Pf1N1B4_4296 - Histidine transporter, periplasmic histidine-binding protein, at 4,759,067 to 4,760,083
_4296
Position (kb)
4758
4759 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 4757.863 kb on + strand at 4757.864 kb on - strand at 4757.864 kb on - strand at 4757.864 kb on - strand at 4757.897 kb on + strand at 4757.973 kb on - strand at 4758.281 kb on + strand at 4758.285 kb on + strand at 4758.286 kb on - strand at 4758.339 kb on + strand at 4758.394 kb on + strand, within Pf1N1B4_4294 at 4758.394 kb on + strand, within Pf1N1B4_4294 at 4758.394 kb on + strand, within Pf1N1B4_4294 at 4758.394 kb on + strand, within Pf1N1B4_4294 at 4758.395 kb on - strand, within Pf1N1B4_4294 at 4758.615 kb on + strand, within Pf1N1B4_4294 at 4758.743 kb on - strand at 4758.743 kb on - strand at 4758.870 kb on + strand, within Pf1N1B4_4295 at 4758.870 kb on + strand, within Pf1N1B4_4295 at 4758.871 kb on - strand, within Pf1N1B4_4295 at 4758.891 kb on + strand, within Pf1N1B4_4295 at 4758.892 kb on - strand, within Pf1N1B4_4295 at 4758.945 kb on + strand at 4758.979 kb on - strand at 4758.979 kb on - strand at 4758.997 kb on - strand at 4759.086 kb on + strand at 4759.116 kb on + strand at 4759.250 kb on - strand, within Pf1N1B4_4296 at 4759.486 kb on + strand, within Pf1N1B4_4296 at 4759.506 kb on - strand, within Pf1N1B4_4296 at 4759.628 kb on + strand, within Pf1N1B4_4296 at 4759.629 kb on - strand, within Pf1N1B4_4296 at 4759.629 kb on - strand, within Pf1N1B4_4296 at 4759.629 kb on - strand, within Pf1N1B4_4296 at 4759.712 kb on + strand, within Pf1N1B4_4296 at 4759.713 kb on - strand, within Pf1N1B4_4296 at 4759.713 kb on - strand, within Pf1N1B4_4296 at 4759.715 kb on + strand, within Pf1N1B4_4296 at 4759.889 kb on + strand, within Pf1N1B4_4296
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,757,863 + -0.4 4,757,864 - -1.4 4,757,864 - -1.7 4,757,864 - +1.7 4,757,897 + +0.4 4,757,973 - +1.4 4,758,281 + -1.4 4,758,285 + -1.4 4,758,286 - +1.2 4,758,339 + -1.1 4,758,394 + Pf1N1B4_4294 0.22 -0.4 4,758,394 + Pf1N1B4_4294 0.22 +1.2 4,758,394 + Pf1N1B4_4294 0.22 -1.4 4,758,394 + Pf1N1B4_4294 0.22 -0.1 4,758,395 - Pf1N1B4_4294 0.22 -1.9 4,758,615 + Pf1N1B4_4294 0.71 -0.1 4,758,743 - +2.5 4,758,743 - +1.9 4,758,870 + Pf1N1B4_4295 0.62 -2.3 4,758,870 + Pf1N1B4_4295 0.62 -2.4 4,758,871 - Pf1N1B4_4295 0.62 -1.9 4,758,891 + Pf1N1B4_4295 0.72 +1.2 4,758,892 - Pf1N1B4_4295 0.73 -1.4 4,758,945 + +2.2 4,758,979 - -0.5 4,758,979 - -1.1 4,758,997 - -0.7 4,759,086 + -1.9 4,759,116 + +0.4 4,759,250 - Pf1N1B4_4296 0.18 +0.6 4,759,486 + Pf1N1B4_4296 0.41 -0.6 4,759,506 - Pf1N1B4_4296 0.43 +0.7 4,759,628 + Pf1N1B4_4296 0.55 -0.7 4,759,629 - Pf1N1B4_4296 0.55 +1.5 4,759,629 - Pf1N1B4_4296 0.55 +1.9 4,759,629 - Pf1N1B4_4296 0.55 +0.5 4,759,712 + Pf1N1B4_4296 0.63 -0.8 4,759,713 - Pf1N1B4_4296 0.64 -0.1 4,759,713 - Pf1N1B4_4296 0.64 +0.3 4,759,715 + Pf1N1B4_4296 0.64 -2.1 4,759,889 + Pf1N1B4_4296 0.81 +0.9
Or see this region's nucleotide sequence