Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4263 and Pf1N1B4_4264 are separated by 448 nucleotides Pf1N1B4_4264 and Pf1N1B4_4265 are separated by 224 nucleotides Pf1N1B4_4265 and Pf1N1B4_4266 are separated by 154 nucleotides
Pf1N1B4_4263 - hypothetical protein, at 4,726,839 to 4,727,135
_4263
Pf1N1B4_4264 - Aminobutyraldehyde dehydrogenase (EC 1.2.1.19), at 4,727,584 to 4,728,783
_4264
Pf1N1B4_4265 - hypothetical protein, at 4,729,008 to 4,729,514
_4265
Pf1N1B4_4266 - sensor histidine kinase, at 4,729,669 to 4,731,063
_4266
Position (kb)
4727
4728
4729 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4726.592 kb on - strand at 4726.608 kb on - strand at 4726.643 kb on + strand at 4726.643 kb on + strand at 4726.664 kb on + strand at 4727.244 kb on - strand at 4727.262 kb on - strand at 4727.290 kb on - strand at 4727.290 kb on - strand at 4727.430 kb on + strand at 4727.453 kb on + strand at 4727.453 kb on + strand at 4727.583 kb on + strand at 4727.583 kb on + strand at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.830 kb on + strand, within Pf1N1B4_4264 at 4727.831 kb on - strand, within Pf1N1B4_4264 at 4727.831 kb on - strand, within Pf1N1B4_4264 at 4728.530 kb on + strand at 4728.530 kb on + strand, within Pf1N1B4_4264 at 4728.530 kb on + strand, within Pf1N1B4_4264 at 4728.531 kb on - strand at 4728.538 kb on + strand, within Pf1N1B4_4264 at 4728.595 kb on + strand, within Pf1N1B4_4264 at 4728.758 kb on + strand at 4728.758 kb on + strand at 4728.761 kb on + strand at 4728.842 kb on - strand at 4728.842 kb on - strand at 4728.908 kb on + strand at 4728.908 kb on + strand at 4728.908 kb on + strand at 4729.018 kb on + strand at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.157 kb on + strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand, within Pf1N1B4_4265 at 4729.158 kb on - strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.161 kb on + strand, within Pf1N1B4_4265 at 4729.162 kb on - strand, within Pf1N1B4_4265 at 4729.162 kb on - strand, within Pf1N1B4_4265 at 4729.416 kb on + strand, within Pf1N1B4_4265 at 4729.443 kb on + strand, within Pf1N1B4_4265 at 4729.443 kb on + strand, within Pf1N1B4_4265 at 4729.444 kb on - strand, within Pf1N1B4_4265 at 4729.444 kb on - strand, within Pf1N1B4_4265 at 4729.595 kb on + strand at 4729.645 kb on - strand at 4729.645 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,726,592 - -1.4 4,726,608 - -1.7 4,726,643 + -0.3 4,726,643 + +1.9 4,726,664 + -1.6 4,727,244 - -1.4 4,727,262 - -2.1 4,727,290 - +0.9 4,727,290 - -0.6 4,727,430 + -2.9 4,727,453 + +1.5 4,727,453 + +0.3 4,727,583 + -1.4 4,727,583 + +0.0 4,727,830 + Pf1N1B4_4264 0.20 -1.5 4,727,830 + Pf1N1B4_4264 0.20 -1.1 4,727,830 + Pf1N1B4_4264 0.20 +0.6 4,727,830 + Pf1N1B4_4264 0.20 +0.9 4,727,831 - Pf1N1B4_4264 0.21 -0.7 4,727,831 - Pf1N1B4_4264 0.21 -0.1 4,728,530 + -0.1 4,728,530 + Pf1N1B4_4264 0.79 -0.7 4,728,530 + Pf1N1B4_4264 0.79 -0.7 4,728,531 - +0.9 4,728,538 + Pf1N1B4_4264 0.80 +0.9 4,728,595 + Pf1N1B4_4264 0.84 -1.1 4,728,758 + +0.5 4,728,758 + -2.4 4,728,761 + -1.1 4,728,842 - -1.1 4,728,842 - -0.2 4,728,908 + -1.1 4,728,908 + +0.4 4,728,908 + -1.7 4,729,018 + +0.2 4,729,157 + Pf1N1B4_4265 0.29 -0.8 4,729,157 + Pf1N1B4_4265 0.29 -0.4 4,729,157 + Pf1N1B4_4265 0.29 +0.9 4,729,158 - Pf1N1B4_4265 0.30 -1.2 4,729,158 - Pf1N1B4_4265 0.30 -0.2 4,729,158 - +0.9 4,729,158 - Pf1N1B4_4265 0.30 +2.1 4,729,158 - Pf1N1B4_4265 0.30 +0.9 4,729,158 - +1.3 4,729,161 + Pf1N1B4_4265 0.30 -0.7 4,729,161 + Pf1N1B4_4265 0.30 -1.1 4,729,161 + Pf1N1B4_4265 0.30 +0.5 4,729,161 + -1.1 4,729,161 + Pf1N1B4_4265 0.30 +0.7 4,729,161 + Pf1N1B4_4265 0.30 -0.1 4,729,161 + Pf1N1B4_4265 0.30 -1.1 4,729,161 + +0.3 4,729,161 + Pf1N1B4_4265 0.30 -0.8 4,729,161 + Pf1N1B4_4265 0.30 +0.5 4,729,162 - Pf1N1B4_4265 0.30 -0.7 4,729,162 - Pf1N1B4_4265 0.30 -1.3 4,729,416 + Pf1N1B4_4265 0.80 +0.5 4,729,443 + Pf1N1B4_4265 0.86 -1.1 4,729,443 + Pf1N1B4_4265 0.86 +0.5 4,729,444 - Pf1N1B4_4265 0.86 -1.1 4,729,444 - Pf1N1B4_4265 0.86 -0.7 4,729,595 + -1.4 4,729,645 - -1.8 4,729,645 - -0.1
Or see this region's nucleotide sequence