Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4244

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4243 and Pf1N1B4_4244 are separated by 3 nucleotidesPf1N1B4_4244 and Pf1N1B4_4245 are separated by 7 nucleotides Pf1N1B4_4243 - Alcohol dehydrogenase (EC 1.1.1.1), at 4,702,373 to 4,703,533 _4243 Pf1N1B4_4244 - Aldehyde dehydrogenase (EC 1.2.1.3), at 4,703,537 to 4,704,928 _4244 Pf1N1B4_4245 - glutamine synthetase family protein, at 4,704,936 to 4,706,309 _4245 Position (kb) 4703 4704 4705Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 4702.602 kb on + strand, within Pf1N1B4_4243at 4702.655 kb on + strandat 4702.655 kb on + strand, within Pf1N1B4_4243at 4702.655 kb on + strand, within Pf1N1B4_4243at 4702.655 kb on + strand, within Pf1N1B4_4243at 4702.656 kb on - strand, within Pf1N1B4_4243at 4702.656 kb on - strand, within Pf1N1B4_4243at 4702.659 kb on + strandat 4702.660 kb on - strandat 4702.701 kb on + strandat 4702.832 kb on + strand, within Pf1N1B4_4243at 4702.873 kb on + strand, within Pf1N1B4_4243at 4702.873 kb on + strand, within Pf1N1B4_4243at 4702.874 kb on - strand, within Pf1N1B4_4243at 4703.678 kb on + strand, within Pf1N1B4_4244at 4703.678 kb on + strand, within Pf1N1B4_4244at 4703.741 kb on - strand, within Pf1N1B4_4244at 4703.857 kb on + strand, within Pf1N1B4_4244at 4703.857 kb on + strand, within Pf1N1B4_4244at 4703.857 kb on + strand, within Pf1N1B4_4244at 4703.857 kb on + strandat 4703.877 kb on - strand, within Pf1N1B4_4244at 4703.888 kb on + strand, within Pf1N1B4_4244at 4703.889 kb on - strand, within Pf1N1B4_4244at 4704.005 kb on - strand, within Pf1N1B4_4244at 4704.054 kb on + strand, within Pf1N1B4_4244at 4704.055 kb on - strand, within Pf1N1B4_4244at 4704.115 kb on - strand, within Pf1N1B4_4244at 4704.118 kb on + strand, within Pf1N1B4_4244at 4704.118 kb on + strand, within Pf1N1B4_4244at 4704.118 kb on + strand, within Pf1N1B4_4244at 4704.119 kb on - strand, within Pf1N1B4_4244at 4704.119 kb on - strand, within Pf1N1B4_4244at 4704.214 kb on - strand, within Pf1N1B4_4244at 4704.503 kb on - strand, within Pf1N1B4_4244at 4704.526 kb on - strand, within Pf1N1B4_4244at 4704.526 kb on - strand, within Pf1N1B4_4244at 4705.021 kb on + strandat 4705.022 kb on - strandat 4705.022 kb on - strandat 4705.022 kb on - strandat 4705.133 kb on - strandat 4705.133 kb on - strand, within Pf1N1B4_4245at 4705.220 kb on - strand, within Pf1N1B4_4245at 4705.320 kb on + strand, within Pf1N1B4_4245at 4705.364 kb on - strand, within Pf1N1B4_4245at 4705.376 kb on - strand, within Pf1N1B4_4245at 4705.786 kb on + strand, within Pf1N1B4_4245at 4705.787 kb on - strand, within Pf1N1B4_4245at 4705.798 kb on + strand, within Pf1N1B4_4245at 4705.798 kb on + strand, within Pf1N1B4_4245at 4705.826 kb on - strand, within Pf1N1B4_4245at 4705.826 kb on - strand, within Pf1N1B4_4245at 4705.849 kb on + strand, within Pf1N1B4_4245at 4705.849 kb on + strand, within Pf1N1B4_4245at 4705.849 kb on + strand, within Pf1N1B4_4245at 4705.849 kb on + strand, within Pf1N1B4_4245at 4705.850 kb on - strand, within Pf1N1B4_4245at 4705.850 kb on - strand, within Pf1N1B4_4245at 4705.867 kb on + strand, within Pf1N1B4_4245at 4705.867 kb on + strandat 4705.868 kb on - strand, within Pf1N1B4_4245

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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4,702,602 + Pf1N1B4_4243 0.20 -1.9
4,702,655 + -0.7
4,702,655 + Pf1N1B4_4243 0.24 -1.1
4,702,655 + Pf1N1B4_4243 0.24 -0.1
4,702,655 + Pf1N1B4_4243 0.24 +0.1
4,702,656 - Pf1N1B4_4243 0.24 -1.4
4,702,656 - Pf1N1B4_4243 0.24 -1.1
4,702,659 + -0.1
4,702,660 - -1.4
4,702,701 + +2.7
4,702,832 + Pf1N1B4_4243 0.40 +0.2
4,702,873 + Pf1N1B4_4243 0.43 -0.4
4,702,873 + Pf1N1B4_4243 0.43 -0.6
4,702,874 - Pf1N1B4_4243 0.43 -1.6
4,703,678 + Pf1N1B4_4244 0.10 -0.5
4,703,678 + Pf1N1B4_4244 0.10 +1.6
4,703,741 - Pf1N1B4_4244 0.15 -2.7
4,703,857 + Pf1N1B4_4244 0.23 +1.7
4,703,857 + Pf1N1B4_4244 0.23 -1.9
4,703,857 + Pf1N1B4_4244 0.23 +0.6
4,703,857 + +0.9
4,703,877 - Pf1N1B4_4244 0.24 -1.7
4,703,888 + Pf1N1B4_4244 0.25 +2.7
4,703,889 - Pf1N1B4_4244 0.25 -0.7
4,704,005 - Pf1N1B4_4244 0.34 +2.2
4,704,054 + Pf1N1B4_4244 0.37 -1.9
4,704,055 - Pf1N1B4_4244 0.37 +0.2
4,704,115 - Pf1N1B4_4244 0.42 -0.1
4,704,118 + Pf1N1B4_4244 0.42 -1.1
4,704,118 + Pf1N1B4_4244 0.42 +1.4
4,704,118 + Pf1N1B4_4244 0.42 +0.6
4,704,119 - Pf1N1B4_4244 0.42 -0.1
4,704,119 - Pf1N1B4_4244 0.42 -2.7
4,704,214 - Pf1N1B4_4244 0.49 -2.6
4,704,503 - Pf1N1B4_4244 0.69 +0.5
4,704,526 - Pf1N1B4_4244 0.71 +0.0
4,704,526 - Pf1N1B4_4244 0.71 -1.3
4,705,021 + -1.3
4,705,022 - -0.9
4,705,022 - -1.9
4,705,022 - +2.5
4,705,133 - -0.1
4,705,133 - Pf1N1B4_4245 0.14 +0.7
4,705,220 - Pf1N1B4_4245 0.21 -0.7
4,705,320 + Pf1N1B4_4245 0.28 -2.9
4,705,364 - Pf1N1B4_4245 0.31 -0.1
4,705,376 - Pf1N1B4_4245 0.32 +2.5
4,705,786 + Pf1N1B4_4245 0.62 +0.9
4,705,787 - Pf1N1B4_4245 0.62 +0.4
4,705,798 + Pf1N1B4_4245 0.63 -1.9
4,705,798 + Pf1N1B4_4245 0.63 -1.8
4,705,826 - Pf1N1B4_4245 0.65 -1.1
4,705,826 - Pf1N1B4_4245 0.65 -0.9
4,705,849 + Pf1N1B4_4245 0.66 -0.7
4,705,849 + Pf1N1B4_4245 0.66 -2.1
4,705,849 + Pf1N1B4_4245 0.66 -2.3
4,705,849 + Pf1N1B4_4245 0.66 -2.4
4,705,850 - Pf1N1B4_4245 0.67 -1.7
4,705,850 - Pf1N1B4_4245 0.67 +1.3
4,705,867 + Pf1N1B4_4245 0.68 -1.1
4,705,867 + -0.1
4,705,868 - Pf1N1B4_4245 0.68 +0.2

Or see this region's nucleotide sequence