Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_420 and Pf1N1B4_421 are separated by 14 nucleotides Pf1N1B4_421 and Pf1N1B4_422 are separated by 43 nucleotides Pf1N1B4_422 and Pf1N1B4_423 overlap by 8 nucleotides Pf1N1B4_423 and Pf1N1B4_424 are separated by 83 nucleotides
Pf1N1B4_420 - Putative deoxyribonuclease YcfH, at 489,069 to 489,860
_420
Pf1N1B4_421 - Type IV pilus biogenesis protein PilZ, at 489,875 to 490,231
_421
Pf1N1B4_422 - DNA polymerase III delta prime subunit (EC 2.7.7.7), at 490,275 to 491,261
_422
Pf1N1B4_423 - Thymidylate kinase (EC 2.7.4.9), at 491,254 to 491,622
_423
Pf1N1B4_424 - deoxynucleotide monophosphate kinase, putative, at 491,706 to 492,512
_424
Position (kb) 490
491
492 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.346 kb on + strand, within Pf1N1B4_420 at 489.347 kb on - strand, within Pf1N1B4_420 at 489.468 kb on - strand at 489.468 kb on - strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.562 kb on + strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.589 kb on - strand, within Pf1N1B4_420 at 489.835 kb on - strand at 489.835 kb on - strand at 489.835 kb on - strand at 489.852 kb on + strand at 489.860 kb on + strand at 489.861 kb on - strand at 489.861 kb on - strand at 489.861 kb on - strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.876 kb on + strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.877 kb on - strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.883 kb on + strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 489.884 kb on - strand at 490.098 kb on + strand, within Pf1N1B4_421 at 490.099 kb on - strand at 490.099 kb on - strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.149 kb on + strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand, within Pf1N1B4_421 at 490.150 kb on - strand at 490.159 kb on - strand, within Pf1N1B4_421 at 490.262 kb on + strand at 490.262 kb on + strand at 490.262 kb on + strand at 490.263 kb on - strand at 490.263 kb on - strand at 491.583 kb on - strand, within Pf1N1B4_423 at 491.583 kb on - strand, within Pf1N1B4_423 at 491.611 kb on - strand at 491.623 kb on - strand at 491.623 kb on - strand at 491.623 kb on - strand at 491.623 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 489,277 - Pf1N1B4_420 0.26 +2.1 489,277 - Pf1N1B4_420 0.26 +0.8 489,343 + Pf1N1B4_420 0.35 -0.2 489,343 + Pf1N1B4_420 0.35 +1.6 489,344 - Pf1N1B4_420 0.35 -0.2 489,344 - Pf1N1B4_420 0.35 -0.4 489,344 - Pf1N1B4_420 0.35 +0.3 489,344 - Pf1N1B4_420 0.35 +0.6 489,344 - Pf1N1B4_420 0.35 -2.9 489,344 - Pf1N1B4_420 0.35 +2.2 489,346 + Pf1N1B4_420 0.35 -1.0 489,347 - Pf1N1B4_420 0.35 +1.1 489,468 - +1.3 489,468 - Pf1N1B4_420 0.50 -1.6 489,556 + Pf1N1B4_420 0.61 -0.9 489,556 + Pf1N1B4_420 0.61 -1.7 489,562 + Pf1N1B4_420 0.62 +1.7 489,563 - Pf1N1B4_420 0.62 -1.1 489,563 - Pf1N1B4_420 0.62 +2.1 489,589 - Pf1N1B4_420 0.66 +2.9 489,835 - -1.4 489,835 - +2.5 489,835 - -1.7 489,852 + -1.2 489,860 + +1.1 489,861 - +0.5 489,861 - -0.9 489,861 - -0.4 489,876 + -0.4 489,876 + -1.2 489,876 + -1.4 489,876 + -0.3 489,877 - +0.6 489,877 - +0.9 489,877 - +0.9 489,877 - -2.3 489,877 - -1.2 489,877 - -3.0 489,877 - +0.9 489,877 - +1.3 489,877 - -1.2 489,877 - -0.4 489,883 + +2.6 489,883 + +2.1 489,883 + -1.0 489,883 + +1.4 489,883 + -2.0 489,884 - +1.6 489,884 - +0.6 489,884 - +1.4 489,884 - +1.6 489,884 - +1.1 489,884 - -0.9 489,884 - +1.1 489,884 - -2.0 490,098 + Pf1N1B4_421 0.62 -0.4 490,099 - +1.6 490,099 - Pf1N1B4_421 0.63 -0.7 490,149 + Pf1N1B4_421 0.77 -0.4 490,149 + Pf1N1B4_421 0.77 -0.9 490,149 + Pf1N1B4_421 0.77 -0.3 490,149 + Pf1N1B4_421 0.77 -1.2 490,149 + Pf1N1B4_421 0.77 -0.4 490,150 - Pf1N1B4_421 0.77 -2.0 490,150 - Pf1N1B4_421 0.77 +0.8 490,150 - Pf1N1B4_421 0.77 +0.1 490,150 - Pf1N1B4_421 0.77 +0.1 490,150 - +2.7 490,159 - Pf1N1B4_421 0.80 +0.1 490,262 + +0.1 490,262 + +1.6 490,262 + -0.2 490,263 - -1.7 490,263 - +1.6 491,583 - Pf1N1B4_423 0.89 +1.6 491,583 - Pf1N1B4_423 0.89 +2.5 491,611 - +0.6 491,623 - +1.3 491,623 - +1.5 491,623 - -0.9 491,623 - -0.3
Or see this region's nucleotide sequence