Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_418 and Pf1N1B4_419 are separated by 139 nucleotides Pf1N1B4_419 and Pf1N1B4_420 are separated by 394 nucleotides
Pf1N1B4_418 - Molybdenum cofactor biosynthesis protein MoaA, at 486,928 to 487,896
_418
Pf1N1B4_419 - Transcriptional regulator, TetR family, at 488,036 to 488,674
_419
Pf1N1B4_420 - Putative deoxyribonuclease YcfH, at 489,069 to 489,860
_420
Position (kb) 488
489 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 487.045 kb on - strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.064 kb on + strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.065 kb on - strand, within Pf1N1B4_418 at 487.108 kb on - strand, within Pf1N1B4_418 at 487.120 kb on + strand, within Pf1N1B4_418 at 487.238 kb on + strand, within Pf1N1B4_418 at 487.239 kb on - strand, within Pf1N1B4_418 at 487.239 kb on - strand, within Pf1N1B4_418 at 487.351 kb on + strand, within Pf1N1B4_418 at 487.351 kb on + strand, within Pf1N1B4_418 at 487.352 kb on - strand, within Pf1N1B4_418 at 487.394 kb on - strand, within Pf1N1B4_418 at 487.732 kb on + strand, within Pf1N1B4_418 at 487.732 kb on + strand, within Pf1N1B4_418 at 487.739 kb on + strand, within Pf1N1B4_418 at 487.739 kb on + strand, within Pf1N1B4_418 at 487.740 kb on - strand, within Pf1N1B4_418 at 487.817 kb on + strand at 487.817 kb on + strand at 487.817 kb on + strand at 487.817 kb on + strand at 487.818 kb on - strand at 487.818 kb on - strand at 487.818 kb on - strand at 487.821 kb on + strand at 487.821 kb on + strand at 487.821 kb on + strand at 487.821 kb on + strand at 487.822 kb on - strand at 487.822 kb on - strand at 487.822 kb on - strand at 487.822 kb on - strand at 487.822 kb on - strand at 487.829 kb on + strand at 487.829 kb on + strand at 487.829 kb on + strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.830 kb on - strand at 487.955 kb on + strand at 487.965 kb on + strand at 487.975 kb on + strand at 487.975 kb on + strand at 487.975 kb on + strand at 487.976 kb on - strand at 487.978 kb on - strand at 487.978 kb on - strand at 487.978 kb on - strand at 487.989 kb on - strand at 488.001 kb on - strand at 488.001 kb on - strand at 488.004 kb on + strand at 488.004 kb on + strand at 488.004 kb on + strand at 488.005 kb on - strand at 488.005 kb on - strand at 488.005 kb on - strand at 488.005 kb on - strand at 488.157 kb on + strand, within Pf1N1B4_419 at 488.158 kb on - strand, within Pf1N1B4_419 at 488.208 kb on + strand, within Pf1N1B4_419 at 488.209 kb on - strand, within Pf1N1B4_419 at 488.209 kb on - strand at 488.493 kb on + strand, within Pf1N1B4_419 at 488.494 kb on - strand, within Pf1N1B4_419 at 488.494 kb on - strand, within Pf1N1B4_419 at 488.494 kb on - strand, within Pf1N1B4_419 at 488.669 kb on - strand at 488.674 kb on + strand at 488.674 kb on + strand at 488.712 kb on + strand at 488.712 kb on + strand at 488.713 kb on - strand at 488.713 kb on - strand at 488.755 kb on + strand at 488.755 kb on + strand at 488.755 kb on + strand at 488.756 kb on - strand at 488.756 kb on - strand at 488.756 kb on - strand at 488.756 kb on - strand at 488.836 kb on - strand at 488.851 kb on + strand at 488.851 kb on + strand at 488.870 kb on + strand at 488.880 kb on + strand at 488.880 kb on + strand at 488.880 kb on + strand at 488.930 kb on + strand at 488.956 kb on + strand at 488.957 kb on - strand at 488.962 kb on + strand at 488.962 kb on + strand at 488.962 kb on + strand at 489.071 kb on - strand at 489.146 kb on - strand at 489.189 kb on + strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.190 kb on - strand, within Pf1N1B4_420 at 489.193 kb on + strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.194 kb on - strand, within Pf1N1B4_420 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.277 kb on - strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.343 kb on + strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.344 kb on - strand, within Pf1N1B4_420 at 489.346 kb on + strand, within Pf1N1B4_420 at 489.347 kb on - strand, within Pf1N1B4_420 at 489.468 kb on - strand at 489.468 kb on - strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.556 kb on + strand, within Pf1N1B4_420 at 489.562 kb on + strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.563 kb on - strand, within Pf1N1B4_420 at 489.589 kb on - strand, within Pf1N1B4_420
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 487,045 - Pf1N1B4_418 0.12 +0.2 487,064 + Pf1N1B4_418 0.14 +1.9 487,064 + Pf1N1B4_418 0.14 -0.9 487,064 + Pf1N1B4_418 0.14 -0.3 487,064 + Pf1N1B4_418 0.14 +0.1 487,065 - Pf1N1B4_418 0.14 -0.7 487,065 - Pf1N1B4_418 0.14 +1.1 487,065 - Pf1N1B4_418 0.14 -1.2 487,065 - Pf1N1B4_418 0.14 +0.6 487,065 - Pf1N1B4_418 0.14 -2.3 487,065 - Pf1N1B4_418 0.14 -2.0 487,108 - Pf1N1B4_418 0.19 -1.4 487,120 + Pf1N1B4_418 0.20 -0.1 487,238 + Pf1N1B4_418 0.32 -0.2 487,239 - Pf1N1B4_418 0.32 -0.4 487,239 - Pf1N1B4_418 0.32 +0.9 487,351 + Pf1N1B4_418 0.44 -0.0 487,351 + Pf1N1B4_418 0.44 +0.2 487,352 - Pf1N1B4_418 0.44 -1.7 487,394 - Pf1N1B4_418 0.48 -1.4 487,732 + Pf1N1B4_418 0.83 +1.5 487,732 + Pf1N1B4_418 0.83 +0.9 487,739 + Pf1N1B4_418 0.84 -1.2 487,739 + Pf1N1B4_418 0.84 +1.6 487,740 - Pf1N1B4_418 0.84 +0.7 487,817 + -0.4 487,817 + -1.6 487,817 + -1.4 487,817 + -0.2 487,818 - -0.9 487,818 - +0.1 487,818 - +0.7 487,821 + +0.1 487,821 + +1.1 487,821 + -1.7 487,821 + +1.4 487,822 - +0.4 487,822 - +0.6 487,822 - -2.2 487,822 - -1.2 487,822 - +0.6 487,829 + -0.2 487,829 + +1.6 487,829 + +1.5 487,830 - +0.3 487,830 - +0.8 487,830 - -0.4 487,830 - +0.6 487,830 - -0.2 487,830 - -1.7 487,830 - -1.4 487,955 + -0.4 487,965 + -1.9 487,975 + -1.0 487,975 + +0.6 487,975 + +1.1 487,976 - -0.9 487,978 - +1.1 487,978 - -0.4 487,978 - -0.4 487,989 - +0.6 488,001 - +1.6 488,001 - -0.4 488,004 + -1.4 488,004 + +0.6 488,004 + +1.8 488,005 - +0.1 488,005 - -1.0 488,005 - -0.9 488,005 - -2.2 488,157 + Pf1N1B4_419 0.19 -1.4 488,158 - Pf1N1B4_419 0.19 -0.9 488,208 + Pf1N1B4_419 0.27 -0.9 488,209 - Pf1N1B4_419 0.27 -0.4 488,209 - -0.4 488,493 + Pf1N1B4_419 0.72 +2.5 488,494 - Pf1N1B4_419 0.72 -1.7 488,494 - Pf1N1B4_419 0.72 -2.4 488,494 - Pf1N1B4_419 0.72 -0.4 488,669 - -0.4 488,674 + -1.4 488,674 + +0.6 488,712 + -1.4 488,712 + -2.4 488,713 - -0.4 488,713 - -1.7 488,755 + -2.2 488,755 + -0.2 488,755 + -0.9 488,756 - -0.6 488,756 - +0.6 488,756 - -0.9 488,756 - +2.5 488,836 - +0.6 488,851 + -0.9 488,851 + +2.0 488,870 + -0.6 488,880 + +0.2 488,880 + -0.9 488,880 + -2.0 488,930 + -2.4 488,956 + +0.1 488,957 - -0.9 488,962 + -1.9 488,962 + -1.0 488,962 + +1.6 489,071 - +1.1 489,146 - +0.9 489,189 + Pf1N1B4_420 0.15 -1.9 489,190 - Pf1N1B4_420 0.15 -1.2 489,190 - Pf1N1B4_420 0.15 +0.9 489,190 - Pf1N1B4_420 0.15 +1.6 489,190 - Pf1N1B4_420 0.15 -1.4 489,190 - Pf1N1B4_420 0.15 +0.0 489,193 + Pf1N1B4_420 0.16 +3.1 489,194 - Pf1N1B4_420 0.16 +0.5 489,194 - Pf1N1B4_420 0.16 -1.4 489,194 - Pf1N1B4_420 0.16 -0.6 489,194 - Pf1N1B4_420 0.16 +0.4 489,277 - Pf1N1B4_420 0.26 +2.1 489,277 - Pf1N1B4_420 0.26 +0.8 489,343 + Pf1N1B4_420 0.35 -0.2 489,343 + Pf1N1B4_420 0.35 +1.6 489,344 - Pf1N1B4_420 0.35 -0.2 489,344 - Pf1N1B4_420 0.35 -0.4 489,344 - Pf1N1B4_420 0.35 +0.3 489,344 - Pf1N1B4_420 0.35 +0.6 489,344 - Pf1N1B4_420 0.35 -2.9 489,344 - Pf1N1B4_420 0.35 +2.2 489,346 + Pf1N1B4_420 0.35 -1.0 489,347 - Pf1N1B4_420 0.35 +1.1 489,468 - +1.3 489,468 - Pf1N1B4_420 0.50 -1.6 489,556 + Pf1N1B4_420 0.61 -0.9 489,556 + Pf1N1B4_420 0.61 -1.7 489,562 + Pf1N1B4_420 0.62 +1.7 489,563 - Pf1N1B4_420 0.62 -1.1 489,563 - Pf1N1B4_420 0.62 +2.1 489,589 - Pf1N1B4_420 0.66 +2.9
Or see this region's nucleotide sequence