Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4087 and Pf1N1B4_4088 are separated by 122 nucleotides Pf1N1B4_4088 and Pf1N1B4_4089 are separated by 400 nucleotides Pf1N1B4_4089 and Pf1N1B4_4090 are separated by 646 nucleotides
Pf1N1B4_4087 - MISCELLANEOUS; Unknown, at 4,535,699 to 4,536,073
_4087
Pf1N1B4_4088 - MISCELLANEOUS; Unknown, at 4,536,196 to 4,536,567
_4088
Pf1N1B4_4089 - hypothetical protein, at 4,536,968 to 4,537,324
_4089
Pf1N1B4_4090 - Exoenzymes regulatory protein AepA in lipid-linked oligosaccharide synthesis cluster, at 4,537,971 to 4,539,713
_4090
Position (kb)
4536
4537
4538 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4535.975 kb on - strand, within Pf1N1B4_4087 at 4536.061 kb on - strand at 4536.061 kb on - strand at 4536.074 kb on - strand at 4536.074 kb on - strand at 4536.139 kb on - strand at 4536.200 kb on + strand at 4536.490 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.492 kb on - strand at 4536.492 kb on - strand, within Pf1N1B4_4088 at 4536.557 kb on + strand at 4536.731 kb on - strand at 4536.791 kb on + strand at 4536.791 kb on + strand at 4536.792 kb on - strand at 4536.817 kb on + strand at 4536.853 kb on - strand at 4536.969 kb on + strand at 4536.970 kb on - strand at 4536.977 kb on - strand at 4537.171 kb on + strand, within Pf1N1B4_4089 at 4537.171 kb on + strand, within Pf1N1B4_4089 at 4537.172 kb on - strand, within Pf1N1B4_4089 at 4537.184 kb on - strand, within Pf1N1B4_4089 at 4537.276 kb on - strand, within Pf1N1B4_4089 at 4537.324 kb on + strand at 4537.324 kb on + strand at 4537.325 kb on - strand at 4537.325 kb on - strand at 4537.395 kb on - strand at 4537.406 kb on + strand at 4537.464 kb on + strand at 4537.465 kb on - strand at 4537.466 kb on + strand at 4537.466 kb on + strand at 4537.466 kb on + strand at 4537.467 kb on - strand at 4537.467 kb on - strand at 4537.508 kb on - strand at 4537.517 kb on - strand at 4537.570 kb on + strand at 4537.571 kb on - strand at 4537.571 kb on - strand at 4537.571 kb on - strand at 4537.571 kb on - strand at 4537.571 kb on - strand at 4537.571 kb on - strand at 4537.638 kb on + strand at 4537.668 kb on + strand at 4537.668 kb on + strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.688 kb on - strand at 4537.768 kb on - strand at 4537.768 kb on - strand at 4537.818 kb on + strand at 4537.820 kb on + strand at 4537.820 kb on + strand at 4537.820 kb on + strand at 4537.821 kb on - strand at 4537.951 kb on - strand at 4537.978 kb on + strand at 4537.979 kb on - strand at 4537.979 kb on - strand at 4537.979 kb on - strand at 4537.979 kb on - strand at 4537.979 kb on - strand at 4538.206 kb on + strand, within Pf1N1B4_4090 at 4538.206 kb on + strand, within Pf1N1B4_4090 at 4538.206 kb on + strand, within Pf1N1B4_4090 at 4538.207 kb on - strand, within Pf1N1B4_4090 at 4538.207 kb on - strand, within Pf1N1B4_4090 at 4538.290 kb on + strand, within Pf1N1B4_4090 at 4538.290 kb on + strand, within Pf1N1B4_4090 at 4538.291 kb on - strand, within Pf1N1B4_4090
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,535,975 - Pf1N1B4_4087 0.74 -0.1 4,536,061 - +1.2 4,536,061 - +0.2 4,536,074 - +1.6 4,536,074 - +1.6 4,536,139 - +2.9 4,536,200 + -1.5 4,536,490 - Pf1N1B4_4088 0.79 -1.1 4,536,492 - Pf1N1B4_4088 0.80 +1.8 4,536,492 - Pf1N1B4_4088 0.80 -0.1 4,536,492 - +1.3 4,536,492 - Pf1N1B4_4088 0.80 +0.6 4,536,557 + -1.9 4,536,731 - -0.5 4,536,791 + -0.9 4,536,791 + +0.3 4,536,792 - +0.2 4,536,817 + -0.5 4,536,853 - -2.8 4,536,969 + +1.3 4,536,970 - -2.1 4,536,977 - -1.4 4,537,171 + Pf1N1B4_4089 0.57 -0.7 4,537,171 + Pf1N1B4_4089 0.57 -2.4 4,537,172 - Pf1N1B4_4089 0.57 +0.9 4,537,184 - Pf1N1B4_4089 0.61 -0.1 4,537,276 - Pf1N1B4_4089 0.86 -0.0 4,537,324 + -1.1 4,537,324 + +1.4 4,537,325 - -0.7 4,537,325 - +1.7 4,537,395 - +0.7 4,537,406 + -1.4 4,537,464 + -0.7 4,537,465 - +0.3 4,537,466 + -1.7 4,537,466 + +2.2 4,537,466 + +1.3 4,537,467 - -0.7 4,537,467 - -2.1 4,537,508 - -1.3 4,537,517 - +1.9 4,537,570 + -2.2 4,537,571 - -0.4 4,537,571 - +0.3 4,537,571 - -0.3 4,537,571 - -1.4 4,537,571 - +0.6 4,537,571 - -0.7 4,537,638 + +0.9 4,537,668 + +0.9 4,537,668 + -0.1 4,537,688 - +0.8 4,537,688 - -1.4 4,537,688 - -0.1 4,537,688 - -0.1 4,537,688 - -1.1 4,537,688 - +1.4 4,537,688 - -1.9 4,537,688 - +0.7 4,537,768 - +0.5 4,537,768 - +0.9 4,537,818 + +1.3 4,537,820 + +1.5 4,537,820 + -0.3 4,537,820 + -0.1 4,537,821 - -0.2 4,537,951 - +0.3 4,537,978 + -1.1 4,537,979 - +0.9 4,537,979 - +0.4 4,537,979 - -1.5 4,537,979 - +2.5 4,537,979 - +0.7 4,538,206 + Pf1N1B4_4090 0.13 -0.7 4,538,206 + Pf1N1B4_4090 0.13 -0.2 4,538,206 + Pf1N1B4_4090 0.13 -0.1 4,538,207 - Pf1N1B4_4090 0.14 +1.9 4,538,207 - Pf1N1B4_4090 0.14 +0.9 4,538,290 + Pf1N1B4_4090 0.18 -0.1 4,538,290 + Pf1N1B4_4090 0.18 -1.7 4,538,291 - Pf1N1B4_4090 0.18 +0.9
Or see this region's nucleotide sequence