Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4045 and Pf1N1B4_4046 are separated by 86 nucleotides Pf1N1B4_4046 and Pf1N1B4_4047 are separated by 127 nucleotides Pf1N1B4_4047 and Pf1N1B4_4048 overlap by 4 nucleotides
Pf1N1B4_4045 - FIG002776: hypothetical protein, at 4,497,082 to 4,498,251
_4045
Pf1N1B4_4046 - Adenylosuccinate lyase (EC 4.3.2.2), at 4,498,338 to 4,499,708
_4046
Pf1N1B4_4047 - FIG002903: a protein of unknown function perhaps involved in purine metabolism, at 4,499,836 to 4,500,459
_4047
Pf1N1B4_4048 - tRNA-specific 2-thiouridylase MnmA, at 4,500,456 to 4,501,589
_4048
Position (kb)
4498
4499
4500 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4497.839 kb on + strand, within Pf1N1B4_4045 at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.795 kb on - strand at 4499.811 kb on + strand at 4499.821 kb on + strand at 4499.868 kb on + strand at 4499.950 kb on + strand at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4499.954 kb on - strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.053 kb on + strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.054 kb on - strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.299 kb on + strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047 at 4500.300 kb on - strand, within Pf1N1B4_4047
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,497,839 + Pf1N1B4_4045 0.65 +0.5 4,499,795 - -2.1 4,499,795 - +0.9 4,499,795 - -1.4 4,499,811 + -0.1 4,499,821 + +2.1 4,499,868 + +0.7 4,499,950 + -0.1 4,499,954 - Pf1N1B4_4047 0.19 +0.8 4,499,954 - Pf1N1B4_4047 0.19 +0.6 4,499,954 - Pf1N1B4_4047 0.19 +0.9 4,500,053 + Pf1N1B4_4047 0.35 +1.2 4,500,053 + Pf1N1B4_4047 0.35 +0.4 4,500,054 - Pf1N1B4_4047 0.35 +1.3 4,500,054 - Pf1N1B4_4047 0.35 +0.6 4,500,054 - Pf1N1B4_4047 0.35 -0.2 4,500,299 + Pf1N1B4_4047 0.74 -0.1 4,500,299 + Pf1N1B4_4047 0.74 -1.1 4,500,299 + Pf1N1B4_4047 0.74 +2.9 4,500,300 - Pf1N1B4_4047 0.74 +1.2 4,500,300 - Pf1N1B4_4047 0.74 +1.1
Or see this region's nucleotide sequence