Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_4001

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_4000 and Pf1N1B4_4001 are separated by 15 nucleotidesPf1N1B4_4001 and Pf1N1B4_4002 are separated by 201 nucleotides Pf1N1B4_4000 - Outer membrane component of tripartite multidrug resistance system, at 4,447,986 to 4,449,455 _4000 Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673 _4001 Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404 _4002 Position (kb) 4449 4450 4451Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4449.103 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.124 kb on - strand, within Pf1N1B4_4000at 4449.240 kb on + strand, within Pf1N1B4_4000at 4449.240 kb on + strand, within Pf1N1B4_4000at 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.241 kb on - strandat 4449.241 kb on - strand, within Pf1N1B4_4000at 4449.279 kb on - strand, within Pf1N1B4_4000at 4449.282 kb on + strand, within Pf1N1B4_4000at 4449.282 kb on + strand, within Pf1N1B4_4000at 4449.453 kb on + strandat 4449.453 kb on + strandat 4449.453 kb on + strandat 4449.485 kb on + strandat 4449.485 kb on + strandat 4449.485 kb on + strandat 4449.486 kb on - strandat 4449.486 kb on - strandat 4449.526 kb on + strandat 4449.527 kb on - strandat 4449.831 kb on + strand, within Pf1N1B4_4001at 4449.831 kb on + strand, within Pf1N1B4_4001at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.329 kb on + strand, within Pf1N1B4_4001at 4450.330 kb on - strand, within Pf1N1B4_4001at 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.584 kb on + strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4450.585 kb on - strandat 4451.233 kb on - strand, within Pf1N1B4_4002at 4451.367 kb on + strand, within Pf1N1B4_4002at 4451.367 kb on + strand, within Pf1N1B4_4002at 4451.368 kb on - strandat 4451.368 kb on - strand, within Pf1N1B4_4002at 4451.481 kb on + strand, within Pf1N1B4_4002at 4451.481 kb on + strand, within Pf1N1B4_4002at 4451.482 kb on - strand, within Pf1N1B4_4002

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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4,449,103 - Pf1N1B4_4000 0.76 +2.7
4,449,124 - Pf1N1B4_4000 0.77 -0.3
4,449,124 - Pf1N1B4_4000 0.77 +0.1
4,449,124 - Pf1N1B4_4000 0.77 -1.4
4,449,240 + Pf1N1B4_4000 0.85 -0.6
4,449,240 + Pf1N1B4_4000 0.85 +3.0
4,449,241 - Pf1N1B4_4000 0.85 +0.8
4,449,241 - Pf1N1B4_4000 0.85 -1.6
4,449,241 - -0.0
4,449,241 - Pf1N1B4_4000 0.85 -2.5
4,449,279 - Pf1N1B4_4000 0.88 -1.6
4,449,282 + Pf1N1B4_4000 0.88 -1.5
4,449,282 + Pf1N1B4_4000 0.88 -0.4
4,449,453 + -1.6
4,449,453 + +2.7
4,449,453 + +0.6
4,449,485 + +0.5
4,449,485 + +0.2
4,449,485 + -0.3
4,449,486 - -1.0
4,449,486 - -1.4
4,449,526 + +0.0
4,449,527 - +0.6
4,449,831 + Pf1N1B4_4001 0.30 -0.6
4,449,831 + Pf1N1B4_4001 0.30 -2.0
4,450,329 + Pf1N1B4_4001 0.71 -0.2
4,450,329 + Pf1N1B4_4001 0.71 -1.6
4,450,330 - Pf1N1B4_4001 0.71 -2.0
4,450,584 + -1.9
4,450,584 + -1.6
4,450,584 + +1.3
4,450,584 + -0.0
4,450,585 - +0.7
4,450,585 - -1.0
4,450,585 - -0.6
4,450,585 - -1.6
4,450,585 - +0.4
4,450,585 - +0.5
4,451,233 - Pf1N1B4_4002 0.23 +0.1
4,451,367 + Pf1N1B4_4002 0.32 +1.4
4,451,367 + Pf1N1B4_4002 0.32 -1.4
4,451,368 - -1.0
4,451,368 - Pf1N1B4_4002 0.32 -1.4
4,451,481 + Pf1N1B4_4002 0.40 -0.1
4,451,481 + Pf1N1B4_4002 0.40 +1.6
4,451,482 - Pf1N1B4_4002 0.40 -0.3

Or see this region's nucleotide sequence