Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_4000 and Pf1N1B4_4001 are separated by 15 nucleotides Pf1N1B4_4001 and Pf1N1B4_4002 are separated by 201 nucleotides
Pf1N1B4_4000 - Outer membrane component of tripartite multidrug resistance system, at 4,447,986 to 4,449,455
_4000
Pf1N1B4_4001 - Membrane fusion component of tripartite multidrug resistance system, at 4,449,471 to 4,450,673
_4001
Pf1N1B4_4002 - Inner membrane component of tripartite multidrug resistance system, at 4,450,875 to 4,452,404
_4002
Position (kb)
4449
4450
4451 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 4449.103 kb on - strand, within Pf1N1B4_4000 at 4449.124 kb on - strand, within Pf1N1B4_4000 at 4449.124 kb on - strand, within Pf1N1B4_4000 at 4449.124 kb on - strand, within Pf1N1B4_4000 at 4449.240 kb on + strand, within Pf1N1B4_4000 at 4449.240 kb on + strand, within Pf1N1B4_4000 at 4449.241 kb on - strand, within Pf1N1B4_4000 at 4449.241 kb on - strand, within Pf1N1B4_4000 at 4449.241 kb on - strand at 4449.241 kb on - strand, within Pf1N1B4_4000 at 4449.279 kb on - strand, within Pf1N1B4_4000 at 4449.282 kb on + strand, within Pf1N1B4_4000 at 4449.282 kb on + strand, within Pf1N1B4_4000 at 4449.453 kb on + strand at 4449.453 kb on + strand at 4449.453 kb on + strand at 4449.485 kb on + strand at 4449.485 kb on + strand at 4449.485 kb on + strand at 4449.486 kb on - strand at 4449.486 kb on - strand at 4449.526 kb on + strand at 4449.527 kb on - strand at 4449.831 kb on + strand, within Pf1N1B4_4001 at 4449.831 kb on + strand, within Pf1N1B4_4001 at 4450.329 kb on + strand, within Pf1N1B4_4001 at 4450.329 kb on + strand, within Pf1N1B4_4001 at 4450.330 kb on - strand, within Pf1N1B4_4001 at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.584 kb on + strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4450.585 kb on - strand at 4451.233 kb on - strand, within Pf1N1B4_4002 at 4451.367 kb on + strand, within Pf1N1B4_4002 at 4451.367 kb on + strand, within Pf1N1B4_4002 at 4451.368 kb on - strand at 4451.368 kb on - strand, within Pf1N1B4_4002 at 4451.481 kb on + strand, within Pf1N1B4_4002 at 4451.481 kb on + strand, within Pf1N1B4_4002 at 4451.482 kb on - strand, within Pf1N1B4_4002
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,449,103 - Pf1N1B4_4000 0.76 +2.7 4,449,124 - Pf1N1B4_4000 0.77 -0.3 4,449,124 - Pf1N1B4_4000 0.77 +0.1 4,449,124 - Pf1N1B4_4000 0.77 -1.4 4,449,240 + Pf1N1B4_4000 0.85 -0.6 4,449,240 + Pf1N1B4_4000 0.85 +3.0 4,449,241 - Pf1N1B4_4000 0.85 +0.8 4,449,241 - Pf1N1B4_4000 0.85 -1.6 4,449,241 - -0.0 4,449,241 - Pf1N1B4_4000 0.85 -2.5 4,449,279 - Pf1N1B4_4000 0.88 -1.6 4,449,282 + Pf1N1B4_4000 0.88 -1.5 4,449,282 + Pf1N1B4_4000 0.88 -0.4 4,449,453 + -1.6 4,449,453 + +2.7 4,449,453 + +0.6 4,449,485 + +0.5 4,449,485 + +0.2 4,449,485 + -0.3 4,449,486 - -1.0 4,449,486 - -1.4 4,449,526 + +0.0 4,449,527 - +0.6 4,449,831 + Pf1N1B4_4001 0.30 -0.6 4,449,831 + Pf1N1B4_4001 0.30 -2.0 4,450,329 + Pf1N1B4_4001 0.71 -0.2 4,450,329 + Pf1N1B4_4001 0.71 -1.6 4,450,330 - Pf1N1B4_4001 0.71 -2.0 4,450,584 + -1.9 4,450,584 + -1.6 4,450,584 + +1.3 4,450,584 + -0.0 4,450,585 - +0.7 4,450,585 - -1.0 4,450,585 - -0.6 4,450,585 - -1.6 4,450,585 - +0.4 4,450,585 - +0.5 4,451,233 - Pf1N1B4_4002 0.23 +0.1 4,451,367 + Pf1N1B4_4002 0.32 +1.4 4,451,367 + Pf1N1B4_4002 0.32 -1.4 4,451,368 - -1.0 4,451,368 - Pf1N1B4_4002 0.32 -1.4 4,451,481 + Pf1N1B4_4002 0.40 -0.1 4,451,481 + Pf1N1B4_4002 0.40 +1.6 4,451,482 - Pf1N1B4_4002 0.40 -0.3
Or see this region's nucleotide sequence