Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3971 and Pf1N1B4_3972 are separated by 8 nucleotides Pf1N1B4_3972 and Pf1N1B4_3973 are separated by 6 nucleotides Pf1N1B4_3973 and Pf1N1B4_3974 overlap by 4 nucleotides
Pf1N1B4_3971 - Opine oxidase subunit C, at 4,413,063 to 4,413,353
_3971
Pf1N1B4_3972 - ABC-type spermidine/putrescine transport system, permease component II, at 4,413,362 to 4,414,156
_3972
Pf1N1B4_3973 - ABC-type spermidine/putrescine transport system, permease component I, at 4,414,163 to 4,415,032
_3973
Pf1N1B4_3974 - ABC transporter, ATP-binding protein, at 4,415,029 to 4,416,054
_3974
Position (kb)
4414
4415
4416 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 4413.489 kb on + strand, within Pf1N1B4_3972 at 4413.490 kb on - strand, within Pf1N1B4_3972 at 4413.495 kb on + strand at 4413.495 kb on + strand, within Pf1N1B4_3972 at 4413.495 kb on + strand at 4413.496 kb on - strand, within Pf1N1B4_3972 at 4413.496 kb on - strand, within Pf1N1B4_3972 at 4413.727 kb on - strand, within Pf1N1B4_3972 at 4413.727 kb on - strand, within Pf1N1B4_3972 at 4414.079 kb on + strand at 4414.287 kb on + strand, within Pf1N1B4_3973 at 4414.287 kb on + strand, within Pf1N1B4_3973 at 4414.575 kb on + strand at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.749 kb on + strand, within Pf1N1B4_3973 at 4414.828 kb on - strand, within Pf1N1B4_3973 at 4414.833 kb on + strand, within Pf1N1B4_3973 at 4414.834 kb on - strand, within Pf1N1B4_3973 at 4414.886 kb on - strand at 4414.886 kb on - strand at 4414.886 kb on - strand at 4414.963 kb on - strand at 4414.963 kb on - strand at 4414.963 kb on - strand at 4415.026 kb on + strand at 4415.026 kb on + strand at 4415.026 kb on + strand at 4415.172 kb on - strand, within Pf1N1B4_3974 at 4415.725 kb on + strand at 4415.726 kb on - strand, within Pf1N1B4_3974 at 4415.746 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,413,489 + Pf1N1B4_3972 0.16 -2.0 4,413,490 - Pf1N1B4_3972 0.16 -1.8 4,413,495 + -1.4 4,413,495 + Pf1N1B4_3972 0.17 -2.4 4,413,495 + -1.6 4,413,496 - Pf1N1B4_3972 0.17 -1.8 4,413,496 - Pf1N1B4_3972 0.17 -0.0 4,413,727 - Pf1N1B4_3972 0.46 +0.5 4,413,727 - Pf1N1B4_3972 0.46 +1.0 4,414,079 + -1.4 4,414,287 + Pf1N1B4_3973 0.14 +1.0 4,414,287 + Pf1N1B4_3973 0.14 -1.5 4,414,575 + +1.0 4,414,749 + Pf1N1B4_3973 0.67 -0.8 4,414,749 + Pf1N1B4_3973 0.67 -1.4 4,414,749 + Pf1N1B4_3973 0.67 -1.3 4,414,749 + Pf1N1B4_3973 0.67 -0.0 4,414,828 - Pf1N1B4_3973 0.76 -1.4 4,414,833 + Pf1N1B4_3973 0.77 -2.5 4,414,834 - Pf1N1B4_3973 0.77 -1.9 4,414,886 - -1.6 4,414,886 - +1.0 4,414,886 - -0.6 4,414,963 - -0.6 4,414,963 - +0.4 4,414,963 - -1.6 4,415,026 + +0.7 4,415,026 + +0.3 4,415,026 + -3.0 4,415,172 - Pf1N1B4_3974 0.14 -0.2 4,415,725 + -0.0 4,415,726 - Pf1N1B4_3974 0.68 -2.0 4,415,746 - +2.0
Or see this region's nucleotide sequence