Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3914 and Pf1N1B4_3915 are separated by 5 nucleotides Pf1N1B4_3915 and Pf1N1B4_3916 are separated by 10 nucleotides Pf1N1B4_3916 and Pf1N1B4_3917 are separated by 369 nucleotides
Pf1N1B4_3914 - COG0553: Superfamily II DNA/RNA helicases, SNF2 family, at 4,347,578 to 4,350,268
_3914
Pf1N1B4_3915 - FIG00954981: hypothetical protein, at 4,350,274 to 4,350,849
_3915
Pf1N1B4_3916 - Transcription-repair coupling factor, at 4,350,860 to 4,354,309
_3916
Pf1N1B4_3917 - NADPH-dependent glyceraldehyde-3-phosphate dehydrogenase (EC 1.2.1.13), at 4,354,679 to 4,356,127
_3917
Position (kb)
4350
4351
4352
4353
4354
4355 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 4349.967 kb on + strand, within Pf1N1B4_3914 at 4350.276 kb on - strand at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.608 kb on + strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.609 kb on - strand, within Pf1N1B4_3915 at 4350.770 kb on + strand, within Pf1N1B4_3915 at 4350.770 kb on + strand, within Pf1N1B4_3915 at 4350.817 kb on + strand at 4350.818 kb on - strand at 4350.899 kb on + strand at 4350.900 kb on - strand at 4350.904 kb on + strand at 4350.907 kb on + strand at 4350.907 kb on + strand at 4350.907 kb on + strand at 4350.907 kb on + strand at 4350.907 kb on + strand at 4350.908 kb on - strand at 4350.908 kb on - strand at 4350.972 kb on + strand at 4351.257 kb on + strand, within Pf1N1B4_3916 at 4351.257 kb on + strand, within Pf1N1B4_3916 at 4351.380 kb on + strand, within Pf1N1B4_3916 at 4351.381 kb on - strand at 4351.566 kb on + strand, within Pf1N1B4_3916 at 4351.572 kb on + strand, within Pf1N1B4_3916 at 4351.731 kb on + strand, within Pf1N1B4_3916 at 4351.731 kb on + strand, within Pf1N1B4_3916 at 4351.731 kb on + strand, within Pf1N1B4_3916 at 4351.863 kb on + strand, within Pf1N1B4_3916 at 4351.863 kb on + strand, within Pf1N1B4_3916 at 4351.866 kb on + strand, within Pf1N1B4_3916 at 4351.866 kb on + strand, within Pf1N1B4_3916 at 4351.866 kb on + strand, within Pf1N1B4_3916 at 4351.866 kb on + strand, within Pf1N1B4_3916 at 4351.866 kb on + strand, within Pf1N1B4_3916 at 4351.942 kb on + strand, within Pf1N1B4_3916 at 4351.942 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.307 kb on + strand, within Pf1N1B4_3916 at 4352.308 kb on - strand at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.589 kb on + strand, within Pf1N1B4_3916 at 4352.590 kb on - strand, within Pf1N1B4_3916 at 4352.590 kb on - strand, within Pf1N1B4_3916 at 4352.590 kb on - strand, within Pf1N1B4_3916 at 4352.745 kb on + strand, within Pf1N1B4_3916 at 4352.745 kb on + strand, within Pf1N1B4_3916 at 4352.745 kb on + strand, within Pf1N1B4_3916 at 4352.746 kb on - strand, within Pf1N1B4_3916 at 4352.826 kb on + strand, within Pf1N1B4_3916 at 4352.873 kb on + strand, within Pf1N1B4_3916 at 4352.873 kb on + strand, within Pf1N1B4_3916 at 4352.874 kb on - strand, within Pf1N1B4_3916 at 4353.406 kb on - strand, within Pf1N1B4_3916 at 4353.531 kb on + strand, within Pf1N1B4_3916 at 4353.531 kb on + strand, within Pf1N1B4_3916 at 4353.531 kb on + strand, within Pf1N1B4_3916 at 4353.531 kb on + strand, within Pf1N1B4_3916 at 4353.531 kb on + strand, within Pf1N1B4_3916 at 4353.532 kb on - strand, within Pf1N1B4_3916 at 4353.532 kb on - strand, within Pf1N1B4_3916 at 4353.534 kb on + strand, within Pf1N1B4_3916 at 4353.535 kb on - strand, within Pf1N1B4_3916 at 4353.840 kb on + strand, within Pf1N1B4_3916 at 4353.840 kb on + strand, within Pf1N1B4_3916 at 4353.855 kb on + strand at 4354.267 kb on + strand at 4354.267 kb on + strand at 4354.391 kb on + strand at 4354.391 kb on + strand at 4354.391 kb on + strand at 4354.392 kb on - strand at 4354.392 kb on - strand at 4354.563 kb on + strand at 4354.636 kb on + strand at 4354.636 kb on + strand at 4354.641 kb on + strand at 4354.641 kb on + strand at 4354.641 kb on + strand at 4354.641 kb on + strand at 4354.661 kb on + strand at 4354.661 kb on + strand at 4354.661 kb on + strand at 4354.661 kb on + strand at 4354.661 kb on + strand at 4354.662 kb on - strand at 4354.662 kb on - strand at 4354.662 kb on - strand at 4354.774 kb on + strand at 4354.796 kb on + strand at 4354.796 kb on + strand at 4354.797 kb on - strand at 4354.950 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,349,967 + Pf1N1B4_3914 0.89 +1.5 4,350,276 - -1.6 4,350,608 + Pf1N1B4_3915 0.58 -0.4 4,350,608 + Pf1N1B4_3915 0.58 -2.0 4,350,608 + Pf1N1B4_3915 0.58 -0.3 4,350,608 + Pf1N1B4_3915 0.58 -0.0 4,350,608 + Pf1N1B4_3915 0.58 -1.0 4,350,608 + Pf1N1B4_3915 0.58 +0.4 4,350,608 + Pf1N1B4_3915 0.58 -1.9 4,350,608 + +1.0 4,350,608 + Pf1N1B4_3915 0.58 -0.0 4,350,608 + Pf1N1B4_3915 0.58 -0.0 4,350,608 + Pf1N1B4_3915 0.58 +0.5 4,350,609 - Pf1N1B4_3915 0.58 +0.0 4,350,609 - Pf1N1B4_3915 0.58 -0.3 4,350,609 - Pf1N1B4_3915 0.58 +0.2 4,350,609 - Pf1N1B4_3915 0.58 +1.4 4,350,609 - Pf1N1B4_3915 0.58 +0.1 4,350,609 - Pf1N1B4_3915 0.58 -2.6 4,350,609 - Pf1N1B4_3915 0.58 +1.6 4,350,609 - Pf1N1B4_3915 0.58 +0.8 4,350,609 - Pf1N1B4_3915 0.58 -0.0 4,350,609 - +1.0 4,350,609 - Pf1N1B4_3915 0.58 -1.2 4,350,609 - Pf1N1B4_3915 0.58 -1.7 4,350,609 - Pf1N1B4_3915 0.58 -0.7 4,350,609 - Pf1N1B4_3915 0.58 +1.3 4,350,770 + Pf1N1B4_3915 0.86 -1.0 4,350,770 + Pf1N1B4_3915 0.86 -0.1 4,350,817 + +0.1 4,350,818 - +0.5 4,350,899 + -1.0 4,350,900 - +0.4 4,350,904 + -0.0 4,350,907 + +2.3 4,350,907 + +2.0 4,350,907 + +2.2 4,350,907 + +3.5 4,350,907 + +0.4 4,350,908 - +1.2 4,350,908 - +1.0 4,350,972 + +0.8 4,351,257 + Pf1N1B4_3916 0.12 +0.1 4,351,257 + Pf1N1B4_3916 0.12 +3.3 4,351,380 + Pf1N1B4_3916 0.15 +2.2 4,351,381 - -0.0 4,351,566 + Pf1N1B4_3916 0.20 +3.8 4,351,572 + Pf1N1B4_3916 0.21 +0.3 4,351,731 + Pf1N1B4_3916 0.25 +2.5 4,351,731 + Pf1N1B4_3916 0.25 +2.3 4,351,731 + Pf1N1B4_3916 0.25 +1.2 4,351,863 + Pf1N1B4_3916 0.29 +0.1 4,351,863 + Pf1N1B4_3916 0.29 +0.5 4,351,866 + Pf1N1B4_3916 0.29 +0.5 4,351,866 + Pf1N1B4_3916 0.29 +1.8 4,351,866 + Pf1N1B4_3916 0.29 +2.2 4,351,866 + Pf1N1B4_3916 0.29 +3.2 4,351,866 + Pf1N1B4_3916 0.29 +2.3 4,351,942 + Pf1N1B4_3916 0.31 +2.1 4,351,942 + Pf1N1B4_3916 0.31 +3.1 4,352,307 + Pf1N1B4_3916 0.42 +2.8 4,352,307 + Pf1N1B4_3916 0.42 -1.4 4,352,307 + Pf1N1B4_3916 0.42 +3.0 4,352,307 + Pf1N1B4_3916 0.42 +1.9 4,352,307 + Pf1N1B4_3916 0.42 -1.4 4,352,307 + Pf1N1B4_3916 0.42 +2.4 4,352,307 + Pf1N1B4_3916 0.42 +2.4 4,352,307 + Pf1N1B4_3916 0.42 +3.2 4,352,308 - +1.0 4,352,589 + Pf1N1B4_3916 0.50 +1.3 4,352,589 + Pf1N1B4_3916 0.50 +2.2 4,352,589 + +1.0 4,352,589 + Pf1N1B4_3916 0.50 +2.3 4,352,589 + Pf1N1B4_3916 0.50 +1.6 4,352,589 + Pf1N1B4_3916 0.50 +1.0 4,352,589 + Pf1N1B4_3916 0.50 +1.1 4,352,589 + Pf1N1B4_3916 0.50 +1.6 4,352,589 + Pf1N1B4_3916 0.50 +2.1 4,352,590 - Pf1N1B4_3916 0.50 +1.3 4,352,590 - Pf1N1B4_3916 0.50 -0.0 4,352,590 - Pf1N1B4_3916 0.50 +2.2 4,352,745 + Pf1N1B4_3916 0.55 +3.6 4,352,745 + Pf1N1B4_3916 0.55 +2.6 4,352,745 + Pf1N1B4_3916 0.55 +3.0 4,352,746 - Pf1N1B4_3916 0.55 -1.4 4,352,826 + Pf1N1B4_3916 0.57 -0.1 4,352,873 + Pf1N1B4_3916 0.58 +2.2 4,352,873 + Pf1N1B4_3916 0.58 +2.1 4,352,874 - Pf1N1B4_3916 0.58 -0.6 4,353,406 - Pf1N1B4_3916 0.74 -0.0 4,353,531 + Pf1N1B4_3916 0.77 -0.4 4,353,531 + Pf1N1B4_3916 0.77 +2.3 4,353,531 + Pf1N1B4_3916 0.77 +1.5 4,353,531 + Pf1N1B4_3916 0.77 -0.5 4,353,531 + Pf1N1B4_3916 0.77 +3.2 4,353,532 - Pf1N1B4_3916 0.77 +1.4 4,353,532 - Pf1N1B4_3916 0.77 +1.7 4,353,534 + Pf1N1B4_3916 0.78 -1.4 4,353,535 - Pf1N1B4_3916 0.78 +2.8 4,353,840 + Pf1N1B4_3916 0.86 +2.8 4,353,840 + Pf1N1B4_3916 0.86 +1.1 4,353,855 + +0.1 4,354,267 + +2.4 4,354,267 + +0.3 4,354,391 + +0.1 4,354,391 + +1.3 4,354,391 + -1.4 4,354,392 - -0.6 4,354,392 - -2.2 4,354,563 + -0.3 4,354,636 + +1.0 4,354,636 + +0.3 4,354,641 + -0.6 4,354,641 + +0.6 4,354,641 + +1.5 4,354,641 + -2.9 4,354,661 + +1.8 4,354,661 + -0.3 4,354,661 + -2.6 4,354,661 + -1.5 4,354,661 + -1.6 4,354,662 - -1.0 4,354,662 - -1.6 4,354,662 - -0.6 4,354,774 + -0.2 4,354,796 + +0.1 4,354,796 + -2.5 4,354,797 - -0.2 4,354,950 + +4.3
Or see this region's nucleotide sequence