Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3827 and Pf1N1B4_3828 are separated by 174 nucleotides Pf1N1B4_3828 and Pf1N1B4_3829 are separated by 25 nucleotides
Pf1N1B4_3827 - Esterase/lipase/thioesterase family protein, at 4,241,897 to 4,242,886
_3827
Pf1N1B4_3828 - Ribonuclease E inhibitor RraA, at 4,243,061 to 4,243,552
_3828
Pf1N1B4_3829 - Magnesium and cobalt transport protein CorA, at 4,243,578 to 4,244,573
_3829
Position (kb)
4243
4244 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 4242.141 kb on - strand, within Pf1N1B4_3827 at 4242.141 kb on - strand, within Pf1N1B4_3827 at 4242.248 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.359 kb on + strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.360 kb on - strand, within Pf1N1B4_3827 at 4242.415 kb on + strand, within Pf1N1B4_3827 at 4242.416 kb on - strand, within Pf1N1B4_3827 at 4242.420 kb on - strand at 4242.754 kb on + strand, within Pf1N1B4_3827 at 4242.898 kb on + strand at 4242.898 kb on + strand at 4242.899 kb on - strand at 4242.899 kb on - strand at 4242.932 kb on + strand at 4242.937 kb on + strand at 4242.938 kb on - strand at 4242.938 kb on - strand at 4242.938 kb on - strand at 4242.938 kb on - strand at 4242.947 kb on + strand at 4243.017 kb on + strand at 4243.018 kb on - strand at 4243.018 kb on - strand at 4243.018 kb on - strand at 4243.046 kb on + strand at 4243.070 kb on + strand at 4243.070 kb on + strand at 4243.070 kb on + strand at 4243.071 kb on - strand at 4243.071 kb on - strand at 4243.097 kb on + strand at 4243.195 kb on + strand, within Pf1N1B4_3828 at 4243.195 kb on + strand at 4243.218 kb on + strand at 4243.218 kb on + strand, within Pf1N1B4_3828 at 4243.237 kb on + strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.341 kb on - strand, within Pf1N1B4_3828 at 4243.448 kb on - strand, within Pf1N1B4_3828 at 4243.448 kb on - strand, within Pf1N1B4_3828 at 4243.490 kb on + strand at 4243.490 kb on + strand, within Pf1N1B4_3828 at 4243.491 kb on - strand at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.491 kb on - strand, within Pf1N1B4_3828 at 4243.497 kb on - strand, within Pf1N1B4_3828 at 4243.551 kb on - strand at 4243.566 kb on - strand at 4243.634 kb on + strand at 4243.751 kb on + strand, within Pf1N1B4_3829 at 4243.751 kb on + strand, within Pf1N1B4_3829 at 4243.751 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.755 kb on + strand, within Pf1N1B4_3829 at 4243.756 kb on - strand, within Pf1N1B4_3829 at 4243.756 kb on - strand, within Pf1N1B4_3829 at 4243.775 kb on + strand, within Pf1N1B4_3829 at 4243.775 kb on + strand, within Pf1N1B4_3829 at 4243.775 kb on + strand, within Pf1N1B4_3829 at 4243.775 kb on + strand, within Pf1N1B4_3829 at 4243.775 kb on + strand, within Pf1N1B4_3829 at 4243.776 kb on - strand, within Pf1N1B4_3829 at 4243.776 kb on - strand, within Pf1N1B4_3829 at 4243.776 kb on - strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.028 kb on + strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.029 kb on - strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.030 kb on + strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.031 kb on - strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.040 kb on + strand, within Pf1N1B4_3829 at 4244.041 kb on - strand at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.041 kb on - strand, within Pf1N1B4_3829 at 4244.049 kb on - strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.129 kb on + strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.130 kb on - strand, within Pf1N1B4_3829 at 4244.207 kb on + strand, within Pf1N1B4_3829 at 4244.208 kb on - strand, within Pf1N1B4_3829 at 4244.230 kb on + strand at 4244.230 kb on + strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.231 kb on - strand, within Pf1N1B4_3829 at 4244.237 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.392 kb on - strand, within Pf1N1B4_3829 at 4244.469 kb on - strand, within Pf1N1B4_3829 at 4244.549 kb on - strand at 4244.549 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,242,141 - Pf1N1B4_3827 0.25 -1.1 4,242,141 - Pf1N1B4_3827 0.25 -1.0 4,242,248 + Pf1N1B4_3827 0.35 +1.6 4,242,359 + Pf1N1B4_3827 0.47 -2.3 4,242,359 + Pf1N1B4_3827 0.47 -1.0 4,242,359 + Pf1N1B4_3827 0.47 -0.8 4,242,359 + Pf1N1B4_3827 0.47 -0.6 4,242,359 + Pf1N1B4_3827 0.47 -1.1 4,242,359 + Pf1N1B4_3827 0.47 -0.6 4,242,359 + Pf1N1B4_3827 0.47 +0.4 4,242,360 - Pf1N1B4_3827 0.47 -1.3 4,242,360 - Pf1N1B4_3827 0.47 -1.2 4,242,360 - Pf1N1B4_3827 0.47 -0.3 4,242,360 - Pf1N1B4_3827 0.47 -1.1 4,242,360 - Pf1N1B4_3827 0.47 +1.0 4,242,360 - Pf1N1B4_3827 0.47 -2.9 4,242,360 - Pf1N1B4_3827 0.47 -1.0 4,242,360 - Pf1N1B4_3827 0.47 -1.6 4,242,360 - Pf1N1B4_3827 0.47 -0.0 4,242,360 - Pf1N1B4_3827 0.47 +2.2 4,242,360 - Pf1N1B4_3827 0.47 -1.0 4,242,360 - Pf1N1B4_3827 0.47 -0.6 4,242,415 + Pf1N1B4_3827 0.52 +1.4 4,242,416 - Pf1N1B4_3827 0.52 -0.0 4,242,420 - -0.0 4,242,754 + Pf1N1B4_3827 0.87 +0.8 4,242,898 + -0.0 4,242,898 + -3.9 4,242,899 - -1.8 4,242,899 - -2.5 4,242,932 + -0.0 4,242,937 + +1.0 4,242,938 - -2.2 4,242,938 - -0.6 4,242,938 - -0.6 4,242,938 - -1.2 4,242,947 + -1.0 4,243,017 + +2.0 4,243,018 - -0.9 4,243,018 - -1.2 4,243,018 - -1.7 4,243,046 + -1.0 4,243,070 + +1.5 4,243,070 + -1.3 4,243,070 + -0.6 4,243,071 - +0.2 4,243,071 - -1.8 4,243,097 + -2.5 4,243,195 + Pf1N1B4_3828 0.27 +2.8 4,243,195 + +2.0 4,243,218 + +1.0 4,243,218 + Pf1N1B4_3828 0.32 -0.7 4,243,237 + Pf1N1B4_3828 0.36 -1.2 4,243,341 - Pf1N1B4_3828 0.57 -2.1 4,243,341 - Pf1N1B4_3828 0.57 -2.3 4,243,341 - Pf1N1B4_3828 0.57 -2.5 4,243,341 - Pf1N1B4_3828 0.57 -3.5 4,243,341 - Pf1N1B4_3828 0.57 -1.0 4,243,341 - Pf1N1B4_3828 0.57 -0.4 4,243,341 - Pf1N1B4_3828 0.57 -1.7 4,243,341 - Pf1N1B4_3828 0.57 +0.2 4,243,341 - Pf1N1B4_3828 0.57 -1.3 4,243,341 - Pf1N1B4_3828 0.57 -3.4 4,243,341 - Pf1N1B4_3828 0.57 -0.8 4,243,341 - +1.6 4,243,341 - Pf1N1B4_3828 0.57 -1.8 4,243,341 - Pf1N1B4_3828 0.57 -0.4 4,243,448 - Pf1N1B4_3828 0.79 -2.3 4,243,448 - Pf1N1B4_3828 0.79 -1.5 4,243,490 + -1.6 4,243,490 + Pf1N1B4_3828 0.87 -0.0 4,243,491 - -0.0 4,243,491 - Pf1N1B4_3828 0.87 -2.0 4,243,491 - Pf1N1B4_3828 0.87 -1.6 4,243,491 - Pf1N1B4_3828 0.87 -2.2 4,243,491 - Pf1N1B4_3828 0.87 -1.8 4,243,491 - Pf1N1B4_3828 0.87 -0.9 4,243,491 - Pf1N1B4_3828 0.87 -1.3 4,243,491 - Pf1N1B4_3828 0.87 -0.6 4,243,491 - Pf1N1B4_3828 0.87 -0.0 4,243,491 - Pf1N1B4_3828 0.87 -3.4 4,243,491 - Pf1N1B4_3828 0.87 -0.8 4,243,491 - Pf1N1B4_3828 0.87 +0.7 4,243,497 - Pf1N1B4_3828 0.89 -1.4 4,243,551 - -1.8 4,243,566 - -1.8 4,243,634 + -0.9 4,243,751 + Pf1N1B4_3829 0.17 +1.8 4,243,751 + Pf1N1B4_3829 0.17 +0.5 4,243,751 + Pf1N1B4_3829 0.17 +0.7 4,243,755 + Pf1N1B4_3829 0.18 -0.6 4,243,755 + Pf1N1B4_3829 0.18 -0.2 4,243,755 + Pf1N1B4_3829 0.18 -1.6 4,243,755 + Pf1N1B4_3829 0.18 -0.0 4,243,755 + Pf1N1B4_3829 0.18 -2.0 4,243,755 + Pf1N1B4_3829 0.18 +0.8 4,243,756 - Pf1N1B4_3829 0.18 -1.8 4,243,756 - Pf1N1B4_3829 0.18 +1.0 4,243,775 + Pf1N1B4_3829 0.20 -2.3 4,243,775 + Pf1N1B4_3829 0.20 -2.8 4,243,775 + Pf1N1B4_3829 0.20 -0.2 4,243,775 + Pf1N1B4_3829 0.20 -1.0 4,243,775 + Pf1N1B4_3829 0.20 -1.1 4,243,776 - Pf1N1B4_3829 0.20 -1.3 4,243,776 - Pf1N1B4_3829 0.20 -1.8 4,243,776 - Pf1N1B4_3829 0.20 -1.8 4,244,028 + Pf1N1B4_3829 0.45 -1.0 4,244,028 + Pf1N1B4_3829 0.45 -0.2 4,244,028 + Pf1N1B4_3829 0.45 -0.6 4,244,028 + Pf1N1B4_3829 0.45 -2.3 4,244,028 + Pf1N1B4_3829 0.45 -2.3 4,244,028 + Pf1N1B4_3829 0.45 -1.6 4,244,028 + Pf1N1B4_3829 0.45 -0.3 4,244,028 + Pf1N1B4_3829 0.45 -0.4 4,244,028 + Pf1N1B4_3829 0.45 +0.6 4,244,028 + Pf1N1B4_3829 0.45 -1.8 4,244,028 + Pf1N1B4_3829 0.45 -0.2 4,244,029 - Pf1N1B4_3829 0.45 -1.6 4,244,029 - Pf1N1B4_3829 0.45 -1.5 4,244,029 - Pf1N1B4_3829 0.45 -1.9 4,244,029 - Pf1N1B4_3829 0.45 -2.3 4,244,029 - Pf1N1B4_3829 0.45 -2.1 4,244,029 - Pf1N1B4_3829 0.45 +3.6 4,244,029 - Pf1N1B4_3829 0.45 -1.6 4,244,029 - Pf1N1B4_3829 0.45 -0.7 4,244,029 - Pf1N1B4_3829 0.45 +0.5 4,244,029 - Pf1N1B4_3829 0.45 -0.5 4,244,029 - Pf1N1B4_3829 0.45 -0.8 4,244,029 - Pf1N1B4_3829 0.45 -3.1 4,244,029 - Pf1N1B4_3829 0.45 -2.2 4,244,029 - Pf1N1B4_3829 0.45 -1.6 4,244,029 - Pf1N1B4_3829 0.45 -1.1 4,244,030 + Pf1N1B4_3829 0.45 -0.0 4,244,030 + Pf1N1B4_3829 0.45 +0.4 4,244,030 + Pf1N1B4_3829 0.45 +0.7 4,244,031 - Pf1N1B4_3829 0.45 -0.6 4,244,031 - Pf1N1B4_3829 0.45 -1.0 4,244,031 - Pf1N1B4_3829 0.45 -2.6 4,244,031 - Pf1N1B4_3829 0.45 +1.0 4,244,031 - Pf1N1B4_3829 0.45 -1.5 4,244,031 - Pf1N1B4_3829 0.45 -2.0 4,244,040 + Pf1N1B4_3829 0.46 -1.0 4,244,040 + Pf1N1B4_3829 0.46 +1.6 4,244,040 + Pf1N1B4_3829 0.46 +2.6 4,244,041 - -1.6 4,244,041 - Pf1N1B4_3829 0.46 -2.0 4,244,041 - Pf1N1B4_3829 0.46 -3.1 4,244,041 - Pf1N1B4_3829 0.46 -3.3 4,244,049 - Pf1N1B4_3829 0.47 -1.0 4,244,129 + Pf1N1B4_3829 0.55 -0.6 4,244,129 + Pf1N1B4_3829 0.55 +0.9 4,244,129 + Pf1N1B4_3829 0.55 -0.0 4,244,130 - Pf1N1B4_3829 0.55 -0.6 4,244,130 - Pf1N1B4_3829 0.55 -2.5 4,244,130 - Pf1N1B4_3829 0.55 -2.1 4,244,130 - Pf1N1B4_3829 0.55 -0.2 4,244,130 - Pf1N1B4_3829 0.55 -3.7 4,244,130 - Pf1N1B4_3829 0.55 +0.1 4,244,130 - -1.3 4,244,130 - Pf1N1B4_3829 0.55 +0.4 4,244,130 - Pf1N1B4_3829 0.55 -0.6 4,244,130 - Pf1N1B4_3829 0.55 -1.0 4,244,207 + Pf1N1B4_3829 0.63 -1.6 4,244,208 - Pf1N1B4_3829 0.63 -1.9 4,244,230 + -0.0 4,244,230 + Pf1N1B4_3829 0.65 +1.4 4,244,231 - Pf1N1B4_3829 0.66 -0.7 4,244,231 - Pf1N1B4_3829 0.66 -0.6 4,244,231 - Pf1N1B4_3829 0.66 -2.1 4,244,231 - Pf1N1B4_3829 0.66 -1.0 4,244,231 - Pf1N1B4_3829 0.66 -1.8 4,244,237 - Pf1N1B4_3829 0.66 -1.3 4,244,392 - Pf1N1B4_3829 0.82 -0.3 4,244,392 - Pf1N1B4_3829 0.82 -3.0 4,244,392 - Pf1N1B4_3829 0.82 -3.5 4,244,392 - Pf1N1B4_3829 0.82 -3.2 4,244,469 - Pf1N1B4_3829 0.89 -2.0 4,244,549 - -0.6 4,244,549 - -3.7
Or see this region's nucleotide sequence