Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3746 and Pf1N1B4_3747 overlap by 4 nucleotides Pf1N1B4_3747 and Pf1N1B4_3748 are separated by 179 nucleotides Pf1N1B4_3748 and Pf1N1B4_3749 are separated by 350 nucleotides
Pf1N1B4_3746 - ABC transporter permease protein, at 4,157,466 to 4,158,311
_3746
Pf1N1B4_3747 - FIG00956482: hypothetical protein, at 4,158,308 to 4,159,114
_3747
Pf1N1B4_3748 - ABC-type Fe3+ transport system, periplasmic component, at 4,159,294 to 4,160,358
_3748
Pf1N1B4_3749 - 2-aminoethylphosphonate uptake and metabolism regulator, at 4,160,709 to 4,161,422
_3749
Position (kb)
4159
4160
4161 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 4158.522 kb on + strand, within Pf1N1B4_3747 at 4158.732 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.810 kb on + strand, within Pf1N1B4_3747 at 4158.811 kb on - strand at 4158.831 kb on + strand, within Pf1N1B4_3747 at 4158.871 kb on - strand, within Pf1N1B4_3747 at 4158.876 kb on + strand, within Pf1N1B4_3747 at 4158.877 kb on - strand, within Pf1N1B4_3747 at 4158.963 kb on + strand, within Pf1N1B4_3747 at 4158.963 kb on + strand, within Pf1N1B4_3747 at 4159.005 kb on + strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.006 kb on - strand, within Pf1N1B4_3747 at 4159.032 kb on + strand at 4159.071 kb on + strand at 4159.149 kb on + strand at 4159.149 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.221 kb on + strand at 4159.222 kb on - strand at 4159.296 kb on - strand at 4159.395 kb on - strand at 4159.395 kb on - strand at 4159.395 kb on - strand at 4159.395 kb on - strand at 4159.511 kb on + strand, within Pf1N1B4_3748 at 4159.511 kb on + strand, within Pf1N1B4_3748 at 4159.512 kb on - strand, within Pf1N1B4_3748 at 4159.512 kb on - strand, within Pf1N1B4_3748 at 4159.512 kb on - strand, within Pf1N1B4_3748 at 4159.512 kb on - strand, within Pf1N1B4_3748 at 4159.512 kb on - strand, within Pf1N1B4_3748 at 4159.550 kb on + strand, within Pf1N1B4_3748 at 4159.551 kb on - strand, within Pf1N1B4_3748 at 4159.551 kb on - strand, within Pf1N1B4_3748 at 4159.613 kb on + strand, within Pf1N1B4_3748 at 4159.613 kb on + strand, within Pf1N1B4_3748 at 4159.614 kb on - strand, within Pf1N1B4_3748 at 4159.614 kb on - strand, within Pf1N1B4_3748 at 4159.643 kb on + strand, within Pf1N1B4_3748 at 4159.643 kb on + strand, within Pf1N1B4_3748 at 4159.643 kb on + strand, within Pf1N1B4_3748 at 4159.644 kb on - strand, within Pf1N1B4_3748 at 4159.644 kb on - strand, within Pf1N1B4_3748 at 4159.644 kb on - strand, within Pf1N1B4_3748 at 4159.816 kb on + strand, within Pf1N1B4_3748 at 4159.869 kb on - strand, within Pf1N1B4_3748 at 4159.958 kb on + strand, within Pf1N1B4_3748 at 4159.958 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.025 kb on + strand, within Pf1N1B4_3748 at 4160.026 kb on - strand, within Pf1N1B4_3748 at 4160.026 kb on - strand, within Pf1N1B4_3748 at 4160.027 kb on + strand at 4160.028 kb on + strand, within Pf1N1B4_3748 at 4160.174 kb on + strand at 4160.175 kb on - strand, within Pf1N1B4_3748 at 4160.175 kb on - strand, within Pf1N1B4_3748 at 4160.420 kb on + strand at 4160.421 kb on - strand at 4160.421 kb on - strand at 4160.540 kb on + strand at 4160.540 kb on + strand at 4160.540 kb on + strand at 4160.613 kb on + strand at 4160.613 kb on + strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.614 kb on - strand at 4160.624 kb on + strand at 4160.625 kb on - strand at 4160.697 kb on + strand at 4160.863 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4160.940 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.129 kb on + strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.130 kb on - strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.141 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.207 kb on + strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand, within Pf1N1B4_3749 at 4161.208 kb on - strand at 4161.339 kb on + strand, within Pf1N1B4_3749
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 4,158,522 + Pf1N1B4_3747 0.27 -0.5 4,158,732 + Pf1N1B4_3747 0.53 +0.5 4,158,810 + Pf1N1B4_3747 0.62 +0.4 4,158,810 + Pf1N1B4_3747 0.62 +0.1 4,158,810 + Pf1N1B4_3747 0.62 +0.1 4,158,810 + Pf1N1B4_3747 0.62 -0.0 4,158,810 + Pf1N1B4_3747 0.62 -2.6 4,158,810 + Pf1N1B4_3747 0.62 -1.7 4,158,810 + Pf1N1B4_3747 0.62 -0.3 4,158,811 - -0.9 4,158,831 + Pf1N1B4_3747 0.65 -0.4 4,158,871 - Pf1N1B4_3747 0.70 -1.7 4,158,876 + Pf1N1B4_3747 0.70 +0.9 4,158,877 - Pf1N1B4_3747 0.71 -0.3 4,158,963 + Pf1N1B4_3747 0.81 -0.5 4,158,963 + Pf1N1B4_3747 0.81 +1.5 4,159,005 + Pf1N1B4_3747 0.86 +1.4 4,159,006 - Pf1N1B4_3747 0.86 +1.1 4,159,006 - Pf1N1B4_3747 0.86 -1.2 4,159,006 - Pf1N1B4_3747 0.86 -0.5 4,159,032 + +2.5 4,159,071 + +2.5 4,159,149 + +0.6 4,159,149 + +1.1 4,159,221 + -0.7 4,159,221 + -0.6 4,159,221 + +1.1 4,159,221 + -0.2 4,159,222 - -0.5 4,159,296 - +0.1 4,159,395 - +0.1 4,159,395 - +1.3 4,159,395 - -0.7 4,159,395 - -0.2 4,159,511 + Pf1N1B4_3748 0.20 -0.5 4,159,511 + Pf1N1B4_3748 0.20 +1.6 4,159,512 - Pf1N1B4_3748 0.20 +0.1 4,159,512 - Pf1N1B4_3748 0.20 -0.4 4,159,512 - Pf1N1B4_3748 0.20 +0.1 4,159,512 - Pf1N1B4_3748 0.20 -2.1 4,159,512 - Pf1N1B4_3748 0.20 -0.9 4,159,550 + Pf1N1B4_3748 0.24 -0.5 4,159,551 - Pf1N1B4_3748 0.24 +1.1 4,159,551 - Pf1N1B4_3748 0.24 +0.2 4,159,613 + Pf1N1B4_3748 0.30 -0.9 4,159,613 + Pf1N1B4_3748 0.30 +1.1 4,159,614 - Pf1N1B4_3748 0.30 -0.9 4,159,614 - Pf1N1B4_3748 0.30 -1.1 4,159,643 + Pf1N1B4_3748 0.33 +0.8 4,159,643 + Pf1N1B4_3748 0.33 -0.7 4,159,643 + Pf1N1B4_3748 0.33 -0.3 4,159,644 - Pf1N1B4_3748 0.33 +0.1 4,159,644 - Pf1N1B4_3748 0.33 -2.8 4,159,644 - Pf1N1B4_3748 0.33 +1.5 4,159,816 + Pf1N1B4_3748 0.49 +0.0 4,159,869 - Pf1N1B4_3748 0.54 -0.3 4,159,958 + Pf1N1B4_3748 0.62 +3.0 4,159,958 + Pf1N1B4_3748 0.62 +1.5 4,160,025 + Pf1N1B4_3748 0.69 -0.5 4,160,025 + Pf1N1B4_3748 0.69 +0.4 4,160,025 + Pf1N1B4_3748 0.69 +1.3 4,160,025 + Pf1N1B4_3748 0.69 +0.6 4,160,026 - Pf1N1B4_3748 0.69 +0.5 4,160,026 - Pf1N1B4_3748 0.69 +0.9 4,160,027 + +0.4 4,160,028 + Pf1N1B4_3748 0.69 -1.2 4,160,174 + -1.2 4,160,175 - Pf1N1B4_3748 0.83 +3.1 4,160,175 - Pf1N1B4_3748 0.83 +0.4 4,160,420 + -0.4 4,160,421 - -1.5 4,160,421 - +0.8 4,160,540 + +0.4 4,160,540 + +2.2 4,160,540 + -2.1 4,160,613 + +0.7 4,160,613 + +0.4 4,160,614 - -1.7 4,160,614 - +0.2 4,160,614 - +1.9 4,160,624 + +0.1 4,160,625 - -0.1 4,160,697 + +0.6 4,160,863 + Pf1N1B4_3749 0.22 -0.2 4,160,940 + Pf1N1B4_3749 0.32 +0.1 4,160,940 + Pf1N1B4_3749 0.32 -0.3 4,161,129 + Pf1N1B4_3749 0.59 +2.2 4,161,129 + Pf1N1B4_3749 0.59 +0.5 4,161,129 + Pf1N1B4_3749 0.59 -0.5 4,161,129 + Pf1N1B4_3749 0.59 -0.9 4,161,129 + Pf1N1B4_3749 0.59 -1.9 4,161,129 + Pf1N1B4_3749 0.59 +0.8 4,161,129 + Pf1N1B4_3749 0.59 +0.9 4,161,129 + Pf1N1B4_3749 0.59 +0.3 4,161,130 - Pf1N1B4_3749 0.59 +0.7 4,161,130 - Pf1N1B4_3749 0.59 +1.4 4,161,130 - Pf1N1B4_3749 0.59 +0.1 4,161,130 - Pf1N1B4_3749 0.59 -2.2 4,161,130 - Pf1N1B4_3749 0.59 +0.4 4,161,141 + Pf1N1B4_3749 0.61 -1.4 4,161,141 + Pf1N1B4_3749 0.61 -0.3 4,161,207 + Pf1N1B4_3749 0.70 -0.3 4,161,207 + Pf1N1B4_3749 0.70 -1.7 4,161,208 - Pf1N1B4_3749 0.70 +1.1 4,161,208 - Pf1N1B4_3749 0.70 -1.5 4,161,208 - Pf1N1B4_3749 0.70 +0.4 4,161,208 - +1.1 4,161,339 + Pf1N1B4_3749 0.88 +1.4
Or see this region's nucleotide sequence