Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_364 and Pf1N1B4_365 are separated by 286 nucleotides Pf1N1B4_365 and Pf1N1B4_366 are separated by 12 nucleotides Pf1N1B4_366 and Pf1N1B4_367 are separated by 189 nucleotides
Pf1N1B4_364 - Rhodanese domain protein UPF0176, at 433,048 to 433,989
_364
Pf1N1B4_365 - Cell division protein BolA, at 434,276 to 434,575
_365
Pf1N1B4_366 - FIG00954136: hypothetical protein, at 434,588 to 435,109
_366
Pf1N1B4_367 - Fumarate hydratase class II (EC 4.2.1.2), at 435,299 to 436,696
_367
Position (kb) 434
435
436 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 433.652 kb on + strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.653 kb on - strand, within Pf1N1B4_364 at 433.934 kb on + strand at 433.956 kb on + strand at 433.956 kb on + strand at 433.958 kb on + strand at 433.958 kb on + strand at 433.959 kb on - strand at 433.994 kb on - strand at 433.994 kb on - strand at 434.017 kb on + strand at 434.018 kb on - strand at 434.076 kb on - strand at 434.204 kb on - strand at 434.204 kb on - strand at 434.204 kb on - strand at 434.277 kb on + strand at 434.277 kb on + strand at 434.277 kb on + strand at 434.278 kb on - strand at 434.349 kb on + strand, within Pf1N1B4_365 at 434.349 kb on + strand, within Pf1N1B4_365 at 434.349 kb on + strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.350 kb on - strand, within Pf1N1B4_365 at 434.403 kb on + strand, within Pf1N1B4_365 at 434.404 kb on - strand, within Pf1N1B4_365 at 434.404 kb on - strand, within Pf1N1B4_365 at 434.590 kb on - strand at 434.620 kb on + strand at 434.761 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.763 kb on + strand, within Pf1N1B4_366 at 434.764 kb on - strand, within Pf1N1B4_366 at 434.764 kb on - strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.811 kb on + strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.812 kb on - strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand, within Pf1N1B4_366 at 434.886 kb on + strand at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.887 kb on - strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.967 kb on + strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 434.968 kb on - strand, within Pf1N1B4_366 at 435.085 kb on + strand at 435.124 kb on + strand at 435.124 kb on + strand at 435.124 kb on + strand at 435.125 kb on - strand at 435.166 kb on - strand at 435.209 kb on + strand at 435.210 kb on - strand at 435.210 kb on - strand at 435.268 kb on - strand at 435.286 kb on + strand at 435.287 kb on - strand at 435.287 kb on - strand at 435.336 kb on + strand at 435.337 kb on - strand at 435.337 kb on - strand at 435.356 kb on + strand at 435.854 kb on - strand, within Pf1N1B4_367 at 435.859 kb on + strand, within Pf1N1B4_367 at 435.859 kb on + strand, within Pf1N1B4_367 at 435.859 kb on + strand, within Pf1N1B4_367 at 435.859 kb on + strand, within Pf1N1B4_367 at 435.860 kb on - strand, within Pf1N1B4_367 at 435.880 kb on + strand, within Pf1N1B4_367 at 435.881 kb on - strand, within Pf1N1B4_367 at 435.881 kb on - strand, within Pf1N1B4_367 at 435.881 kb on - strand, within Pf1N1B4_367 at 436.073 kb on - strand, within Pf1N1B4_367 at 436.084 kb on + strand, within Pf1N1B4_367 at 436.084 kb on + strand, within Pf1N1B4_367 at 436.084 kb on + strand, within Pf1N1B4_367
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 433,652 + Pf1N1B4_364 0.64 +0.1 433,653 - Pf1N1B4_364 0.64 -1.9 433,653 - Pf1N1B4_364 0.64 -0.5 433,934 + +0.1 433,956 + -0.0 433,956 + -0.9 433,958 + +1.4 433,958 + +2.7 433,959 - +0.5 433,994 - +2.7 433,994 - -1.5 434,017 + -0.5 434,018 - -0.9 434,076 - -0.0 434,204 - +1.9 434,204 - +0.5 434,204 - +0.4 434,277 + -0.9 434,277 + -1.5 434,277 + -0.9 434,278 - -1.9 434,349 + Pf1N1B4_365 0.24 -2.0 434,349 + Pf1N1B4_365 0.24 +1.5 434,349 + Pf1N1B4_365 0.24 +0.5 434,350 - Pf1N1B4_365 0.25 +1.4 434,350 - Pf1N1B4_365 0.25 +1.8 434,350 - Pf1N1B4_365 0.25 +2.7 434,350 - Pf1N1B4_365 0.25 +1.0 434,350 - Pf1N1B4_365 0.25 +1.6 434,350 - Pf1N1B4_365 0.25 +0.2 434,350 - Pf1N1B4_365 0.25 +3.1 434,350 - Pf1N1B4_365 0.25 -0.3 434,350 - Pf1N1B4_365 0.25 -0.4 434,350 - Pf1N1B4_365 0.25 +3.7 434,350 - Pf1N1B4_365 0.25 -0.1 434,350 - Pf1N1B4_365 0.25 -1.0 434,350 - Pf1N1B4_365 0.25 -0.1 434,403 + Pf1N1B4_365 0.42 +1.9 434,404 - Pf1N1B4_365 0.43 +0.9 434,404 - Pf1N1B4_365 0.43 -1.7 434,590 - +2.6 434,620 + +1.5 434,761 + Pf1N1B4_366 0.33 +1.9 434,763 + Pf1N1B4_366 0.34 +1.3 434,763 + Pf1N1B4_366 0.34 +0.5 434,763 + Pf1N1B4_366 0.34 +2.7 434,763 + Pf1N1B4_366 0.34 +0.8 434,764 - Pf1N1B4_366 0.34 +0.9 434,764 - Pf1N1B4_366 0.34 +0.7 434,811 + Pf1N1B4_366 0.43 +1.2 434,811 + Pf1N1B4_366 0.43 +1.5 434,811 + Pf1N1B4_366 0.43 +1.3 434,811 + Pf1N1B4_366 0.43 +1.7 434,812 - Pf1N1B4_366 0.43 +1.7 434,812 - Pf1N1B4_366 0.43 +2.7 434,812 - Pf1N1B4_366 0.43 +0.1 434,812 - Pf1N1B4_366 0.43 -0.5 434,812 - Pf1N1B4_366 0.43 +1.6 434,812 - Pf1N1B4_366 0.43 +1.4 434,886 + Pf1N1B4_366 0.57 +1.4 434,886 + +2.7 434,886 + Pf1N1B4_366 0.57 +1.6 434,886 + Pf1N1B4_366 0.57 +2.3 434,886 + Pf1N1B4_366 0.57 +0.4 434,886 + +4.6 434,887 - Pf1N1B4_366 0.57 -1.5 434,887 - Pf1N1B4_366 0.57 +2.5 434,887 - Pf1N1B4_366 0.57 +0.7 434,887 - Pf1N1B4_366 0.57 +0.9 434,887 - Pf1N1B4_366 0.57 +0.7 434,887 - Pf1N1B4_366 0.57 +0.9 434,887 - Pf1N1B4_366 0.57 +0.2 434,887 - Pf1N1B4_366 0.57 +0.4 434,967 + Pf1N1B4_366 0.73 +2.5 434,967 + Pf1N1B4_366 0.73 +1.8 434,967 + Pf1N1B4_366 0.73 +1.0 434,967 + Pf1N1B4_366 0.73 +1.8 434,967 + Pf1N1B4_366 0.73 +2.5 434,967 + Pf1N1B4_366 0.73 -0.8 434,967 + Pf1N1B4_366 0.73 +0.5 434,967 + Pf1N1B4_366 0.73 +1.3 434,967 + Pf1N1B4_366 0.73 +1.7 434,967 + Pf1N1B4_366 0.73 +0.9 434,967 + Pf1N1B4_366 0.73 +2.3 434,967 + Pf1N1B4_366 0.73 +1.0 434,968 - Pf1N1B4_366 0.73 +1.1 434,968 - Pf1N1B4_366 0.73 +2.0 434,968 - Pf1N1B4_366 0.73 +1.9 434,968 - Pf1N1B4_366 0.73 -0.9 435,085 + +0.8 435,124 + +1.7 435,124 + +1.7 435,124 + -0.9 435,125 - -0.5 435,166 - -1.9 435,209 + +0.8 435,210 - +0.1 435,210 - +0.1 435,268 - +0.1 435,286 + -0.3 435,287 - -0.9 435,287 - +0.3 435,336 + -2.2 435,337 - -1.2 435,337 - -1.7 435,356 + +1.7 435,854 - Pf1N1B4_367 0.40 +0.1 435,859 + Pf1N1B4_367 0.40 -0.5 435,859 + Pf1N1B4_367 0.40 +1.1 435,859 + Pf1N1B4_367 0.40 -3.0 435,859 + Pf1N1B4_367 0.40 -2.2 435,860 - Pf1N1B4_367 0.40 -3.5 435,880 + Pf1N1B4_367 0.42 -0.6 435,881 - Pf1N1B4_367 0.42 +0.1 435,881 - Pf1N1B4_367 0.42 +1.4 435,881 - Pf1N1B4_367 0.42 -2.2 436,073 - Pf1N1B4_367 0.55 -0.5 436,084 + Pf1N1B4_367 0.56 -1.2 436,084 + Pf1N1B4_367 0.56 -1.2 436,084 + Pf1N1B4_367 0.56 -1.5
Or see this region's nucleotide sequence