Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3482 and Pf1N1B4_3483 are separated by 192 nucleotides Pf1N1B4_3483 and Pf1N1B4_3484 overlap by 4 nucleotides Pf1N1B4_3484 and Pf1N1B4_3485 are separated by 486 nucleotides Pf1N1B4_3485 and Pf1N1B4_3486 are separated by 126 nucleotides
Pf1N1B4_3482 - outer membrane protein H1, at 3,889,223 to 3,889,825
_3482
Pf1N1B4_3483 - Transcriptional regulatory protein PhoP, at 3,890,018 to 3,890,695
_3483
Pf1N1B4_3484 - Sensor protein PhoQ (EC 2.7.13.3), at 3,890,692 to 3,892,038
_3484
Pf1N1B4_3485 - hypothetical protein, at 3,892,525 to 3,892,884
_3485
Pf1N1B4_3486 - Transcriptional regulator, AraC family, at 3,893,011 to 3,894,024
_3486
Position (kb)
3890
3891
3892
3893 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 3889.712 kb on + strand, within Pf1N1B4_3482 at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.799 kb on + strand at 3889.800 kb on - strand at 3889.814 kb on + strand at 3889.814 kb on + strand at 3889.814 kb on + strand at 3889.814 kb on + strand at 3889.814 kb on + strand at 3889.823 kb on + strand at 3889.823 kb on + strand at 3889.823 kb on + strand at 3889.824 kb on - strand at 3889.824 kb on - strand at 3889.835 kb on + strand at 3889.835 kb on + strand at 3889.836 kb on - strand at 3889.980 kb on + strand at 3889.980 kb on + strand at 3889.980 kb on + strand at 3890.017 kb on + strand at 3890.129 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.309 kb on + strand, within Pf1N1B4_3483 at 3890.310 kb on - strand, within Pf1N1B4_3483 at 3890.475 kb on - strand, within Pf1N1B4_3483 at 3890.495 kb on + strand, within Pf1N1B4_3483 at 3890.495 kb on + strand, within Pf1N1B4_3483 at 3890.496 kb on - strand, within Pf1N1B4_3483 at 3890.496 kb on - strand, within Pf1N1B4_3483 at 3890.938 kb on + strand, within Pf1N1B4_3484 at 3890.938 kb on + strand, within Pf1N1B4_3484 at 3890.939 kb on - strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.944 kb on + strand, within Pf1N1B4_3484 at 3890.945 kb on - strand, within Pf1N1B4_3484 at 3891.004 kb on + strand, within Pf1N1B4_3484 at 3891.004 kb on + strand, within Pf1N1B4_3484 at 3891.079 kb on + strand, within Pf1N1B4_3484 at 3891.079 kb on + strand, within Pf1N1B4_3484 at 3891.079 kb on + strand, within Pf1N1B4_3484 at 3891.709 kb on + strand, within Pf1N1B4_3484 at 3891.709 kb on + strand, within Pf1N1B4_3484 at 3891.709 kb on + strand, within Pf1N1B4_3484 at 3891.709 kb on + strand, within Pf1N1B4_3484 at 3891.709 kb on + strand, within Pf1N1B4_3484 at 3891.964 kb on + strand at 3891.964 kb on + strand at 3892.163 kb on + strand at 3892.178 kb on - strand at 3892.194 kb on + strand at 3892.235 kb on + strand at 3892.277 kb on + strand at 3892.277 kb on + strand at 3892.277 kb on + strand at 3892.278 kb on - strand at 3892.371 kb on + strand at 3892.459 kb on + strand at 3892.474 kb on + strand at 3892.474 kb on + strand at 3892.474 kb on + strand at 3892.475 kb on + strand at 3892.475 kb on - strand at 3892.488 kb on + strand at 3892.537 kb on - strand at 3892.537 kb on - strand at 3892.627 kb on + strand, within Pf1N1B4_3485 at 3892.627 kb on + strand, within Pf1N1B4_3485 at 3892.630 kb on + strand at 3892.634 kb on + strand at 3892.634 kb on + strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485 at 3892.682 kb on - strand, within Pf1N1B4_3485
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,889,712 + Pf1N1B4_3482 0.81 -1.5 3,889,799 + -0.1 3,889,799 + +0.1 3,889,799 + -1.9 3,889,799 + -1.0 3,889,799 + -0.7 3,889,799 + -1.0 3,889,799 + +0.5 3,889,800 - -0.5 3,889,814 + -1.7 3,889,814 + -1.3 3,889,814 + -0.2 3,889,814 + -1.5 3,889,814 + -1.9 3,889,823 + -1.2 3,889,823 + -0.1 3,889,823 + -0.5 3,889,824 - -0.6 3,889,824 - +1.1 3,889,835 + -1.0 3,889,835 + +0.9 3,889,836 - -2.0 3,889,980 + +3.2 3,889,980 + +2.5 3,889,980 + +2.0 3,890,017 + -0.3 3,890,129 + Pf1N1B4_3483 0.16 -2.0 3,890,309 + Pf1N1B4_3483 0.43 -0.9 3,890,309 + Pf1N1B4_3483 0.43 -1.5 3,890,309 + Pf1N1B4_3483 0.43 -1.2 3,890,309 + Pf1N1B4_3483 0.43 -1.5 3,890,309 + Pf1N1B4_3483 0.43 -0.2 3,890,309 + Pf1N1B4_3483 0.43 -1.5 3,890,309 + Pf1N1B4_3483 0.43 -0.5 3,890,310 - Pf1N1B4_3483 0.43 -1.9 3,890,475 - Pf1N1B4_3483 0.67 +0.9 3,890,495 + Pf1N1B4_3483 0.70 -1.9 3,890,495 + Pf1N1B4_3483 0.70 +0.1 3,890,496 - Pf1N1B4_3483 0.71 -0.9 3,890,496 - Pf1N1B4_3483 0.71 -1.2 3,890,938 + Pf1N1B4_3484 0.18 -0.9 3,890,938 + Pf1N1B4_3484 0.18 +2.5 3,890,939 - Pf1N1B4_3484 0.18 +2.9 3,890,944 + Pf1N1B4_3484 0.19 +2.2 3,890,944 + Pf1N1B4_3484 0.19 +2.3 3,890,944 + Pf1N1B4_3484 0.19 +0.5 3,890,944 + Pf1N1B4_3484 0.19 -0.9 3,890,944 + Pf1N1B4_3484 0.19 +0.7 3,890,944 + Pf1N1B4_3484 0.19 +0.4 3,890,944 + Pf1N1B4_3484 0.19 -0.2 3,890,944 + Pf1N1B4_3484 0.19 +3.3 3,890,944 + Pf1N1B4_3484 0.19 +0.1 3,890,945 - Pf1N1B4_3484 0.19 -0.9 3,891,004 + Pf1N1B4_3484 0.23 -0.1 3,891,004 + Pf1N1B4_3484 0.23 +3.1 3,891,079 + Pf1N1B4_3484 0.29 +2.5 3,891,079 + Pf1N1B4_3484 0.29 +1.0 3,891,079 + Pf1N1B4_3484 0.29 -1.5 3,891,709 + Pf1N1B4_3484 0.76 +2.9 3,891,709 + Pf1N1B4_3484 0.76 +3.4 3,891,709 + Pf1N1B4_3484 0.76 +3.1 3,891,709 + Pf1N1B4_3484 0.76 +3.6 3,891,709 + Pf1N1B4_3484 0.76 +1.8 3,891,964 + +4.3 3,891,964 + +2.8 3,892,163 + +1.1 3,892,178 - +1.5 3,892,194 + +1.9 3,892,235 + +1.1 3,892,277 + +0.4 3,892,277 + +1.1 3,892,277 + +1.4 3,892,278 - +0.1 3,892,371 + -0.1 3,892,459 + -1.7 3,892,474 + +1.4 3,892,474 + +0.1 3,892,474 + +1.6 3,892,475 + +1.1 3,892,475 - +1.4 3,892,488 + -0.6 3,892,537 - +1.1 3,892,537 - -0.7 3,892,627 + Pf1N1B4_3485 0.28 +1.9 3,892,627 + Pf1N1B4_3485 0.28 +1.7 3,892,630 + -0.5 3,892,634 + +1.9 3,892,634 + Pf1N1B4_3485 0.30 +0.9 3,892,682 - Pf1N1B4_3485 0.44 -0.5 3,892,682 - Pf1N1B4_3485 0.44 -1.2 3,892,682 - Pf1N1B4_3485 0.44 -0.5
Or see this region's nucleotide sequence