Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3416 and Pf1N1B4_3417 are separated by 359 nucleotides Pf1N1B4_3417 and Pf1N1B4_3418 are separated by 138 nucleotides Pf1N1B4_3418 and Pf1N1B4_3419 are separated by 23 nucleotides
Pf1N1B4_3416 - Outer membrane protein romA, at 3,805,873 to 3,806,931
_3416
Pf1N1B4_3417 - FIG00964635: hypothetical protein, at 3,807,291 to 3,807,608
_3417
Pf1N1B4_3418 - hypothetical protein, at 3,807,747 to 3,807,860
_3418
Pf1N1B4_3419 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 3,807,884 to 3,810,175
_3419
Position (kb)
3807
3808 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3806.335 kb on + strand, within Pf1N1B4_3416 at 3806.336 kb on - strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.641 kb on + strand, within Pf1N1B4_3416 at 3806.642 kb on - strand, within Pf1N1B4_3416 at 3806.707 kb on - strand, within Pf1N1B4_3416 at 3806.707 kb on - strand, within Pf1N1B4_3416 at 3806.784 kb on + strand, within Pf1N1B4_3416 at 3806.857 kb on + strand at 3806.857 kb on + strand at 3806.858 kb on - strand at 3806.858 kb on - strand at 3806.858 kb on - strand at 3806.983 kb on + strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.984 kb on - strand at 3806.999 kb on + strand at 3807.000 kb on - strand at 3807.026 kb on + strand at 3807.026 kb on + strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.027 kb on - strand at 3807.085 kb on + strand at 3807.085 kb on + strand at 3807.097 kb on + strand at 3807.098 kb on - strand at 3807.098 kb on - strand at 3807.189 kb on + strand at 3807.307 kb on + strand at 3807.308 kb on - strand at 3807.749 kb on - strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.894 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.903 kb on + strand at 3807.904 kb on - strand at 3807.904 kb on - strand at 3807.987 kb on + strand at 3808.065 kb on + strand at 3808.103 kb on + strand at 3808.104 kb on - strand at 3808.197 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.335 kb on + strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.336 kb on - strand, within Pf1N1B4_3419 at 3808.394 kb on + strand, within Pf1N1B4_3419 at 3808.395 kb on - strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.496 kb on + strand, within Pf1N1B4_3419 at 3808.497 kb on - strand, within Pf1N1B4_3419 at 3808.497 kb on - strand, within Pf1N1B4_3419 at 3808.523 kb on + strand, within Pf1N1B4_3419 at 3808.523 kb on + strand, within Pf1N1B4_3419 at 3808.565 kb on + strand, within Pf1N1B4_3419 at 3808.577 kb on + strand, within Pf1N1B4_3419
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,806,335 + Pf1N1B4_3416 0.44 +0.7 3,806,336 - Pf1N1B4_3416 0.44 -0.1 3,806,641 + Pf1N1B4_3416 0.73 +1.8 3,806,641 + Pf1N1B4_3416 0.73 -1.9 3,806,642 - Pf1N1B4_3416 0.73 -0.7 3,806,707 - Pf1N1B4_3416 0.79 +0.1 3,806,707 - Pf1N1B4_3416 0.79 +0.0 3,806,784 + Pf1N1B4_3416 0.86 +0.0 3,806,857 + -1.7 3,806,857 + +1.1 3,806,858 - -1.3 3,806,858 - -0.9 3,806,858 - +0.1 3,806,983 + -1.7 3,806,984 - -0.5 3,806,984 - +0.8 3,806,984 - +0.9 3,806,999 + -2.3 3,807,000 - -1.3 3,807,026 + +1.8 3,807,026 + +0.8 3,807,027 - +1.7 3,807,027 - -0.9 3,807,027 - +1.6 3,807,085 + +1.0 3,807,085 + -1.2 3,807,097 + -2.3 3,807,098 - -1.5 3,807,098 - -0.3 3,807,189 + +0.3 3,807,307 + -0.5 3,807,308 - +2.7 3,807,749 - -0.6 3,807,894 + -1.2 3,807,894 + -1.9 3,807,894 + +0.8 3,807,903 + -0.3 3,807,903 + -1.6 3,807,903 + +0.4 3,807,903 + -1.7 3,807,904 - +1.5 3,807,904 - -0.3 3,807,987 + +0.1 3,808,065 + +0.1 3,808,103 + -1.2 3,808,104 - -0.2 3,808,197 + Pf1N1B4_3419 0.14 +0.8 3,808,335 + Pf1N1B4_3419 0.20 -0.5 3,808,335 + Pf1N1B4_3419 0.20 -2.5 3,808,335 + +2.1 3,808,335 + Pf1N1B4_3419 0.20 +1.8 3,808,335 + Pf1N1B4_3419 0.20 -2.0 3,808,335 + Pf1N1B4_3419 0.20 -1.2 3,808,336 - Pf1N1B4_3419 0.20 -0.8 3,808,336 - Pf1N1B4_3419 0.20 -0.6 3,808,336 - Pf1N1B4_3419 0.20 -1.2 3,808,336 - Pf1N1B4_3419 0.20 -1.5 3,808,336 - Pf1N1B4_3419 0.20 +0.5 3,808,394 + Pf1N1B4_3419 0.22 +0.1 3,808,395 - Pf1N1B4_3419 0.22 -0.2 3,808,496 + Pf1N1B4_3419 0.27 +0.3 3,808,496 + Pf1N1B4_3419 0.27 -1.8 3,808,496 + Pf1N1B4_3419 0.27 -0.3 3,808,496 + Pf1N1B4_3419 0.27 -0.3 3,808,497 - Pf1N1B4_3419 0.27 +0.0 3,808,497 - Pf1N1B4_3419 0.27 +2.9 3,808,523 + Pf1N1B4_3419 0.28 -1.9 3,808,523 + Pf1N1B4_3419 0.28 +0.3 3,808,565 + Pf1N1B4_3419 0.30 -1.2 3,808,577 + Pf1N1B4_3419 0.30 +0.4
Or see this region's nucleotide sequence