Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3411 and Pf1N1B4_3412 overlap by 4 nucleotides Pf1N1B4_3412 and Pf1N1B4_3413 are separated by 78 nucleotides Pf1N1B4_3413 and Pf1N1B4_3414 are separated by 6 nucleotides Pf1N1B4_3414 and Pf1N1B4_3415 are separated by 141 nucleotides
Pf1N1B4_3411 - FIG00959431: hypothetical protein, at 3,802,668 to 3,802,991
_3411
Pf1N1B4_3412 - CTP synthase (EC 6.3.4.2), at 3,802,988 to 3,803,695
_3412
Pf1N1B4_3413 - Transcriptional regulator, LysR family, at 3,803,774 to 3,804,652
_3413
Pf1N1B4_3414 - PROBABLE MEMBRANE PROTEIN, at 3,804,659 to 3,804,907
_3414
Pf1N1B4_3415 - Transcriptional regulator, TetR family, at 3,805,049 to 3,805,657
_3415
Position (kb)
3803
3804
3805 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 3802.811 kb on - strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.820 kb on + strand, within Pf1N1B4_3411 at 3802.946 kb on + strand, within Pf1N1B4_3411 at 3802.947 kb on - strand, within Pf1N1B4_3411 at 3802.947 kb on - strand, within Pf1N1B4_3411 at 3802.960 kb on + strand at 3802.964 kb on - strand at 3802.964 kb on - strand at 3803.051 kb on - strand at 3803.105 kb on + strand, within Pf1N1B4_3412 at 3803.105 kb on - strand, within Pf1N1B4_3412 at 3803.168 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.579 kb on + strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.580 kb on - strand, within Pf1N1B4_3412 at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.734 kb on + strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.735 kb on - strand at 3803.808 kb on - strand at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.308 kb on + strand, within Pf1N1B4_3413 at 3804.439 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand, within Pf1N1B4_3413 at 3804.465 kb on + strand at 3804.817 kb on + strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.818 kb on - strand, within Pf1N1B4_3414 at 3804.944 kb on + strand at 3804.945 kb on - strand at 3804.946 kb on + strand at 3804.947 kb on - strand at 3804.990 kb on + strand at 3804.991 kb on - strand at 3804.991 kb on - strand at 3804.995 kb on - strand at 3805.060 kb on - strand at 3805.060 kb on - strand at 3805.248 kb on - strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.515 kb on + strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.516 kb on - strand, within Pf1N1B4_3415 at 3805.528 kb on + strand, within Pf1N1B4_3415
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,802,811 - Pf1N1B4_3411 0.44 -0.9 3,802,820 + Pf1N1B4_3411 0.47 +1.3 3,802,820 + Pf1N1B4_3411 0.47 +0.1 3,802,820 + Pf1N1B4_3411 0.47 -0.2 3,802,946 + Pf1N1B4_3411 0.86 -1.9 3,802,947 - Pf1N1B4_3411 0.86 -0.5 3,802,947 - Pf1N1B4_3411 0.86 +0.1 3,802,960 + +0.1 3,802,964 - -2.3 3,802,964 - +2.6 3,803,051 - +0.4 3,803,105 + Pf1N1B4_3412 0.17 -2.5 3,803,105 - Pf1N1B4_3412 0.17 -0.2 3,803,168 + Pf1N1B4_3412 0.25 -0.7 3,803,579 + Pf1N1B4_3412 0.83 -1.6 3,803,579 + Pf1N1B4_3412 0.83 -1.9 3,803,579 + Pf1N1B4_3412 0.83 +1.5 3,803,579 + Pf1N1B4_3412 0.83 -1.5 3,803,579 + Pf1N1B4_3412 0.83 +2.0 3,803,580 - Pf1N1B4_3412 0.84 -1.0 3,803,580 - Pf1N1B4_3412 0.84 -0.7 3,803,580 - Pf1N1B4_3412 0.84 -0.2 3,803,580 - Pf1N1B4_3412 0.84 -2.9 3,803,580 - Pf1N1B4_3412 0.84 -1.9 3,803,734 + +0.0 3,803,734 + -1.5 3,803,734 + -1.5 3,803,734 + +0.8 3,803,734 + +1.1 3,803,735 - -1.9 3,803,735 - +0.4 3,803,735 - -0.5 3,803,735 - +1.1 3,803,735 - -0.9 3,803,735 - +0.1 3,803,735 - -0.2 3,803,735 - +0.8 3,803,808 - -1.2 3,804,308 + Pf1N1B4_3413 0.61 +2.9 3,804,308 + Pf1N1B4_3413 0.61 +1.1 3,804,439 + Pf1N1B4_3413 0.76 -0.9 3,804,465 + Pf1N1B4_3413 0.79 +0.5 3,804,465 + -0.9 3,804,817 + Pf1N1B4_3414 0.63 -1.0 3,804,818 - Pf1N1B4_3414 0.64 +3.1 3,804,818 - Pf1N1B4_3414 0.64 +0.7 3,804,818 - Pf1N1B4_3414 0.64 -0.6 3,804,944 + +3.5 3,804,945 - +0.1 3,804,946 + -0.6 3,804,947 - -0.9 3,804,990 + -1.2 3,804,991 - +0.1 3,804,991 - +0.7 3,804,995 - -1.0 3,805,060 - -0.5 3,805,060 - +0.4 3,805,248 - Pf1N1B4_3415 0.33 +1.8 3,805,515 + Pf1N1B4_3415 0.77 +3.1 3,805,515 + Pf1N1B4_3415 0.77 +2.7 3,805,515 + Pf1N1B4_3415 0.77 +3.8 3,805,516 - Pf1N1B4_3415 0.77 +1.3 3,805,516 - Pf1N1B4_3415 0.77 +1.3 3,805,516 - Pf1N1B4_3415 0.77 -0.2 3,805,528 + Pf1N1B4_3415 0.79 +2.1
Or see this region's nucleotide sequence