Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3390 and Pf1N1B4_3391 overlap by 7 nucleotides Pf1N1B4_3391 and Pf1N1B4_3392 are separated by 231 nucleotides Pf1N1B4_3392 and Pf1N1B4_3393 are separated by 149 nucleotides
Pf1N1B4_3390 - RNA polymerase sigma factor RpoE, at 3,784,250 to 3,784,864
_3390
Pf1N1B4_3391 - FIG00955837: hypothetical protein, at 3,784,858 to 3,785,094
_3391
Pf1N1B4_3392 - Pyrophosphate-specific outer membrane porin OprO, at 3,785,326 to 3,786,669
_3392
Pf1N1B4_3393 - COGs COG3146, at 3,786,819 to 3,787,943
_3393
Position (kb)
3785
3786
3787 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3784.331 kb on + strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.332 kb on - strand, within Pf1N1B4_3390 at 3784.359 kb on - strand, within Pf1N1B4_3390 at 3784.359 kb on - strand, within Pf1N1B4_3390 at 3784.897 kb on + strand, within Pf1N1B4_3391 at 3784.897 kb on + strand, within Pf1N1B4_3391 at 3785.033 kb on + strand at 3785.093 kb on - strand at 3785.129 kb on - strand at 3785.129 kb on - strand at 3785.129 kb on - strand at 3785.297 kb on + strand at 3785.297 kb on + strand at 3785.298 kb on - strand at 3785.429 kb on + strand at 3785.512 kb on + strand, within Pf1N1B4_3392 at 3785.513 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.534 kb on - strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.566 kb on + strand, within Pf1N1B4_3392 at 3785.567 kb on - strand, within Pf1N1B4_3392 at 3785.567 kb on - strand, within Pf1N1B4_3392 at 3785.567 kb on - strand at 3785.605 kb on + strand at 3785.605 kb on + strand, within Pf1N1B4_3392 at 3785.605 kb on + strand, within Pf1N1B4_3392 at 3785.606 kb on - strand, within Pf1N1B4_3392 at 3785.606 kb on - strand, within Pf1N1B4_3392 at 3785.620 kb on + strand, within Pf1N1B4_3392 at 3785.621 kb on - strand at 3785.621 kb on - strand at 3785.632 kb on + strand, within Pf1N1B4_3392 at 3785.632 kb on + strand, within Pf1N1B4_3392 at 3785.671 kb on + strand, within Pf1N1B4_3392 at 3785.671 kb on + strand, within Pf1N1B4_3392 at 3785.695 kb on + strand, within Pf1N1B4_3392 at 3785.696 kb on - strand, within Pf1N1B4_3392 at 3785.807 kb on - strand, within Pf1N1B4_3392 at 3785.807 kb on - strand, within Pf1N1B4_3392 at 3785.884 kb on + strand, within Pf1N1B4_3392 at 3785.885 kb on - strand, within Pf1N1B4_3392 at 3785.885 kb on - strand, within Pf1N1B4_3392 at 3786.167 kb on - strand, within Pf1N1B4_3392 at 3786.184 kb on + strand, within Pf1N1B4_3392 at 3786.235 kb on + strand, within Pf1N1B4_3392 at 3786.235 kb on + strand, within Pf1N1B4_3392 at 3786.235 kb on + strand, within Pf1N1B4_3392 at 3786.235 kb on + strand, within Pf1N1B4_3392 at 3786.235 kb on + strand, within Pf1N1B4_3392 at 3786.236 kb on - strand, within Pf1N1B4_3392 at 3786.298 kb on + strand, within Pf1N1B4_3392 at 3786.298 kb on + strand, within Pf1N1B4_3392 at 3786.299 kb on - strand, within Pf1N1B4_3392 at 3786.313 kb on + strand, within Pf1N1B4_3392 at 3786.314 kb on - strand, within Pf1N1B4_3392 at 3786.314 kb on - strand, within Pf1N1B4_3392 at 3786.342 kb on + strand, within Pf1N1B4_3392 at 3786.343 kb on - strand, within Pf1N1B4_3392 at 3786.343 kb on - strand, within Pf1N1B4_3392 at 3786.406 kb on + strand, within Pf1N1B4_3392 at 3786.406 kb on + strand, within Pf1N1B4_3392 at 3786.406 kb on + strand, within Pf1N1B4_3392 at 3786.407 kb on - strand, within Pf1N1B4_3392 at 3786.407 kb on - strand, within Pf1N1B4_3392 at 3786.407 kb on - strand, within Pf1N1B4_3392 at 3786.547 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.658 kb on + strand at 3786.659 kb on - strand at 3786.659 kb on - strand at 3786.659 kb on - strand at 3786.667 kb on + strand at 3786.667 kb on + strand at 3786.667 kb on + strand at 3786.668 kb on - strand at 3786.668 kb on - strand at 3786.686 kb on + strand at 3786.687 kb on - strand at 3786.772 kb on + strand at 3786.773 kb on - strand at 3786.779 kb on + strand at 3786.780 kb on - strand at 3786.794 kb on + strand at 3786.794 kb on + strand at 3786.795 kb on - strand at 3787.047 kb on + strand, within Pf1N1B4_3393 at 3787.047 kb on + strand, within Pf1N1B4_3393 at 3787.048 kb on - strand, within Pf1N1B4_3393 at 3787.048 kb on - strand, within Pf1N1B4_3393 at 3787.098 kb on + strand, within Pf1N1B4_3393 at 3787.098 kb on + strand, within Pf1N1B4_3393 at 3787.099 kb on - strand, within Pf1N1B4_3393 at 3787.190 kb on - strand, within Pf1N1B4_3393 at 3787.366 kb on + strand, within Pf1N1B4_3393 at 3787.367 kb on - strand, within Pf1N1B4_3393 at 3787.367 kb on - strand, within Pf1N1B4_3393 at 3787.367 kb on - strand, within Pf1N1B4_3393 at 3787.482 kb on + strand, within Pf1N1B4_3393 at 3787.492 kb on - strand, within Pf1N1B4_3393 at 3787.499 kb on + strand, within Pf1N1B4_3393 at 3787.499 kb on + strand, within Pf1N1B4_3393 at 3787.500 kb on - strand, within Pf1N1B4_3393 at 3787.503 kb on + strand, within Pf1N1B4_3393 at 3787.503 kb on + strand, within Pf1N1B4_3393 at 3787.504 kb on - strand, within Pf1N1B4_3393 at 3787.504 kb on - strand, within Pf1N1B4_3393 at 3787.552 kb on + strand, within Pf1N1B4_3393 at 3787.552 kb on + strand, within Pf1N1B4_3393 at 3787.653 kb on + strand, within Pf1N1B4_3393 at 3787.653 kb on + strand, within Pf1N1B4_3393
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,784,331 + Pf1N1B4_3390 0.13 +1.7 3,784,332 - Pf1N1B4_3390 0.13 +0.6 3,784,332 - Pf1N1B4_3390 0.13 -0.0 3,784,332 - Pf1N1B4_3390 0.13 -0.4 3,784,359 - Pf1N1B4_3390 0.18 +1.6 3,784,359 - Pf1N1B4_3390 0.18 -1.3 3,784,897 + Pf1N1B4_3391 0.16 +0.6 3,784,897 + Pf1N1B4_3391 0.16 -1.2 3,785,033 + +0.6 3,785,093 - -1.0 3,785,129 - +1.7 3,785,129 - +0.7 3,785,129 - -1.2 3,785,297 + +1.1 3,785,297 + -2.2 3,785,298 - -0.1 3,785,429 + +0.3 3,785,512 + Pf1N1B4_3392 0.14 -0.9 3,785,513 - Pf1N1B4_3392 0.14 +0.1 3,785,534 - Pf1N1B4_3392 0.15 +0.0 3,785,534 - Pf1N1B4_3392 0.15 -0.4 3,785,534 - Pf1N1B4_3392 0.15 +0.1 3,785,534 - Pf1N1B4_3392 0.15 -1.7 3,785,566 + Pf1N1B4_3392 0.18 -0.2 3,785,566 + Pf1N1B4_3392 0.18 +0.6 3,785,566 + Pf1N1B4_3392 0.18 +0.1 3,785,566 + Pf1N1B4_3392 0.18 -1.2 3,785,566 + Pf1N1B4_3392 0.18 -1.7 3,785,567 - Pf1N1B4_3392 0.18 +0.9 3,785,567 - Pf1N1B4_3392 0.18 -2.4 3,785,567 - +3.1 3,785,605 + +1.1 3,785,605 + Pf1N1B4_3392 0.21 +1.1 3,785,605 + Pf1N1B4_3392 0.21 -0.9 3,785,606 - Pf1N1B4_3392 0.21 -0.6 3,785,606 - Pf1N1B4_3392 0.21 +0.3 3,785,620 + Pf1N1B4_3392 0.22 -0.9 3,785,621 - +0.1 3,785,621 - -1.7 3,785,632 + Pf1N1B4_3392 0.23 -2.0 3,785,632 + Pf1N1B4_3392 0.23 +1.7 3,785,671 + Pf1N1B4_3392 0.26 +0.6 3,785,671 + Pf1N1B4_3392 0.26 -0.9 3,785,695 + Pf1N1B4_3392 0.27 -0.4 3,785,696 - Pf1N1B4_3392 0.28 -0.0 3,785,807 - Pf1N1B4_3392 0.36 -0.7 3,785,807 - Pf1N1B4_3392 0.36 +0.2 3,785,884 + Pf1N1B4_3392 0.42 -0.9 3,785,885 - Pf1N1B4_3392 0.42 +0.3 3,785,885 - Pf1N1B4_3392 0.42 +0.1 3,786,167 - Pf1N1B4_3392 0.63 -0.7 3,786,184 + Pf1N1B4_3392 0.64 -0.5 3,786,235 + Pf1N1B4_3392 0.68 -0.0 3,786,235 + Pf1N1B4_3392 0.68 -0.9 3,786,235 + Pf1N1B4_3392 0.68 +2.7 3,786,235 + Pf1N1B4_3392 0.68 -1.4 3,786,235 + Pf1N1B4_3392 0.68 -0.4 3,786,236 - Pf1N1B4_3392 0.68 -0.4 3,786,298 + Pf1N1B4_3392 0.72 +1.1 3,786,298 + Pf1N1B4_3392 0.72 +0.1 3,786,299 - Pf1N1B4_3392 0.72 -2.4 3,786,313 + Pf1N1B4_3392 0.73 +0.4 3,786,314 - Pf1N1B4_3392 0.74 -1.9 3,786,314 - Pf1N1B4_3392 0.74 -1.0 3,786,342 + Pf1N1B4_3392 0.76 +0.1 3,786,343 - Pf1N1B4_3392 0.76 -1.2 3,786,343 - Pf1N1B4_3392 0.76 -0.1 3,786,406 + Pf1N1B4_3392 0.80 -0.9 3,786,406 + Pf1N1B4_3392 0.80 -0.4 3,786,406 + Pf1N1B4_3392 0.80 -1.2 3,786,407 - Pf1N1B4_3392 0.80 +2.0 3,786,407 - Pf1N1B4_3392 0.80 -1.4 3,786,407 - Pf1N1B4_3392 0.80 +0.4 3,786,547 + +0.4 3,786,658 + +0.7 3,786,658 + -0.6 3,786,658 + -0.2 3,786,658 + -1.4 3,786,658 + +0.8 3,786,658 + +0.1 3,786,658 + +0.1 3,786,658 + -0.4 3,786,658 + +0.7 3,786,658 + +1.0 3,786,659 - +1.2 3,786,659 - -0.0 3,786,659 - +1.4 3,786,667 + +0.2 3,786,667 + +1.4 3,786,667 + -0.2 3,786,668 - -1.9 3,786,668 - +0.1 3,786,686 + +0.1 3,786,687 - -1.0 3,786,772 + +0.7 3,786,773 - +0.1 3,786,779 + -1.2 3,786,780 - -1.7 3,786,794 + -1.7 3,786,794 + +2.7 3,786,795 - -1.9 3,787,047 + Pf1N1B4_3393 0.20 -0.4 3,787,047 + Pf1N1B4_3393 0.20 -2.9 3,787,048 - Pf1N1B4_3393 0.20 -0.9 3,787,048 - Pf1N1B4_3393 0.20 -0.9 3,787,098 + Pf1N1B4_3393 0.25 -1.9 3,787,098 + Pf1N1B4_3393 0.25 -0.4 3,787,099 - Pf1N1B4_3393 0.25 -0.7 3,787,190 - Pf1N1B4_3393 0.33 -0.9 3,787,366 + Pf1N1B4_3393 0.49 +0.7 3,787,367 - Pf1N1B4_3393 0.49 -0.1 3,787,367 - Pf1N1B4_3393 0.49 -0.4 3,787,367 - Pf1N1B4_3393 0.49 -1.4 3,787,482 + Pf1N1B4_3393 0.59 +1.5 3,787,492 - Pf1N1B4_3393 0.60 -1.2 3,787,499 + Pf1N1B4_3393 0.60 -1.7 3,787,499 + Pf1N1B4_3393 0.60 -0.1 3,787,500 - Pf1N1B4_3393 0.61 -1.7 3,787,503 + Pf1N1B4_3393 0.61 -2.9 3,787,503 + Pf1N1B4_3393 0.61 +3.1 3,787,504 - Pf1N1B4_3393 0.61 +2.1 3,787,504 - Pf1N1B4_3393 0.61 -0.4 3,787,552 + Pf1N1B4_3393 0.65 +1.8 3,787,552 + Pf1N1B4_3393 0.65 -0.2 3,787,653 + Pf1N1B4_3393 0.74 +1.0 3,787,653 + Pf1N1B4_3393 0.74 -0.7
Or see this region's nucleotide sequence