Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3370 and Pf1N1B4_3371 are separated by 244 nucleotides Pf1N1B4_3371 and Pf1N1B4_3372 overlap by 13 nucleotides Pf1N1B4_3372 and Pf1N1B4_3373 overlap by 4 nucleotides Pf1N1B4_3373 and Pf1N1B4_3374 are separated by 126 nucleotides
Pf1N1B4_3370 - ATPase associated with various cellular activities, AAA_5, at 3,758,058 to 3,758,903
_3370
Pf1N1B4_3371 - FIG00954979: hypothetical protein, at 3,759,148 to 3,759,336
_3371
Pf1N1B4_3372 - FIG00962464: hypothetical protein, at 3,759,324 to 3,759,827
_3372
Pf1N1B4_3373 - FIG00954689: hypothetical protein, at 3,759,824 to 3,760,900
_3373
Pf1N1B4_3374 - Cysteine synthase (EC 2.5.1.47), at 3,761,027 to 3,762,001
_3374
Position (kb)
3759
3760
3761 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3758.878 kb on + strand at 3758.879 kb on - strand at 3758.879 kb on - strand at 3758.957 kb on + strand at 3758.957 kb on + strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.958 kb on - strand at 3758.963 kb on + strand at 3758.963 kb on + strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.964 kb on - strand at 3758.967 kb on - strand at 3758.974 kb on + strand at 3758.974 kb on + strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3758.975 kb on - strand at 3759.013 kb on + strand at 3759.014 kb on - strand at 3759.014 kb on - strand at 3759.068 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.111 kb on + strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.112 kb on - strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.114 kb on + strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.115 kb on - strand at 3759.119 kb on - strand at 3759.133 kb on - strand at 3759.328 kb on + strand at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.561 kb on + strand, within Pf1N1B4_3372 at 3759.562 kb on - strand, within Pf1N1B4_3372 at 3759.589 kb on + strand, within Pf1N1B4_3372 at 3759.606 kb on - strand, within Pf1N1B4_3372 at 3759.683 kb on + strand at 3759.920 kb on - strand at 3760.108 kb on - strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.377 kb on + strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.378 kb on - strand, within Pf1N1B4_3373 at 3760.617 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.748 kb on + strand, within Pf1N1B4_3373 at 3760.818 kb on + strand at 3760.819 kb on - strand at 3760.888 kb on - strand at 3760.920 kb on + strand at 3760.923 kb on - strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.986 kb on + strand at 3760.987 kb on - strand at 3760.987 kb on - strand at 3760.999 kb on + strand at 3760.999 kb on + strand at 3761.000 kb on - strand at 3761.000 kb on - strand at 3761.012 kb on + strand at 3761.012 kb on + strand at 3761.012 kb on + strand at 3761.035 kb on + strand at 3761.035 kb on + strand at 3761.036 kb on - strand at 3761.036 kb on - strand at 3761.036 kb on - strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.039 kb on + strand at 3761.040 kb on - strand at 3761.040 kb on - strand at 3761.040 kb on - strand at 3761.068 kb on + strand at 3761.068 kb on + strand at 3761.069 kb on - strand at 3761.298 kb on + strand, within Pf1N1B4_3374 at 3761.444 kb on + strand, within Pf1N1B4_3374 at 3761.444 kb on + strand, within Pf1N1B4_3374 at 3761.445 kb on - strand, within Pf1N1B4_3374 at 3761.445 kb on - strand, within Pf1N1B4_3374 at 3761.445 kb on - strand at 3761.544 kb on + strand at 3761.544 kb on + strand, within Pf1N1B4_3374 at 3761.544 kb on + strand, within Pf1N1B4_3374 at 3761.545 kb on - strand, within Pf1N1B4_3374 at 3761.595 kb on - strand, within Pf1N1B4_3374 at 3761.596 kb on + strand at 3761.597 kb on - strand, within Pf1N1B4_3374 at 3761.843 kb on - strand, within Pf1N1B4_3374 at 3761.843 kb on - strand, within Pf1N1B4_3374
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,758,878 + -0.2 3,758,879 - -1.4 3,758,879 - -1.7 3,758,957 + -1.0 3,758,957 + -0.9 3,758,958 - -1.4 3,758,958 - -0.8 3,758,958 - +1.3 3,758,963 + -1.7 3,758,963 + -0.9 3,758,964 - -0.9 3,758,964 - -0.6 3,758,964 - +1.1 3,758,967 - -1.3 3,758,974 + -1.4 3,758,974 + -0.9 3,758,975 - -1.7 3,758,975 - -0.4 3,758,975 - -0.2 3,758,975 - -1.6 3,759,013 + -0.4 3,759,014 - -1.1 3,759,014 - +0.7 3,759,068 + +0.8 3,759,111 + -1.2 3,759,111 + -1.2 3,759,111 + -0.7 3,759,111 + +1.1 3,759,112 - -1.7 3,759,112 - -2.0 3,759,112 - -2.0 3,759,114 + -0.3 3,759,114 + +2.7 3,759,114 + +0.0 3,759,114 + +1.4 3,759,114 + -0.4 3,759,114 + +2.7 3,759,114 + -1.7 3,759,114 + +0.1 3,759,114 + -0.3 3,759,114 + -1.4 3,759,114 + -2.3 3,759,115 - -1.7 3,759,115 - -0.3 3,759,115 - +0.9 3,759,115 - +1.4 3,759,115 - +0.9 3,759,115 - -1.0 3,759,115 - -1.9 3,759,115 - -0.7 3,759,115 - +2.6 3,759,115 - -1.2 3,759,115 - +2.0 3,759,119 - +0.8 3,759,133 - -0.4 3,759,328 + +1.1 3,759,561 + Pf1N1B4_3372 0.47 -0.2 3,759,561 + Pf1N1B4_3372 0.47 -2.6 3,759,562 - Pf1N1B4_3372 0.47 -3.2 3,759,589 + Pf1N1B4_3372 0.53 -0.3 3,759,606 - Pf1N1B4_3372 0.56 -1.2 3,759,683 + +3.1 3,759,920 - -1.4 3,760,108 - Pf1N1B4_3373 0.26 +0.7 3,760,377 + Pf1N1B4_3373 0.51 -1.7 3,760,377 + Pf1N1B4_3373 0.51 -2.2 3,760,378 - Pf1N1B4_3373 0.51 -2.1 3,760,378 - Pf1N1B4_3373 0.51 +0.1 3,760,617 + Pf1N1B4_3373 0.74 -2.4 3,760,748 + -0.9 3,760,748 + Pf1N1B4_3373 0.86 -1.2 3,760,748 + Pf1N1B4_3373 0.86 +0.6 3,760,748 + Pf1N1B4_3373 0.86 -1.2 3,760,818 + -0.4 3,760,819 - +1.1 3,760,888 - +0.1 3,760,920 + +0.9 3,760,923 - -0.3 3,760,986 + +0.1 3,760,986 + +0.7 3,760,986 + -0.4 3,760,986 + -1.4 3,760,987 - +0.6 3,760,987 - -0.6 3,760,999 + -2.6 3,760,999 + -0.9 3,761,000 - +1.1 3,761,000 - +1.0 3,761,012 + -0.1 3,761,012 + +0.3 3,761,012 + +0.8 3,761,035 + +0.1 3,761,035 + -2.2 3,761,036 - +1.1 3,761,036 - -1.7 3,761,036 - +0.1 3,761,039 + -2.2 3,761,039 + +0.5 3,761,039 + +2.0 3,761,039 + -0.4 3,761,039 + -2.0 3,761,039 + +0.1 3,761,039 + -2.6 3,761,040 - -0.9 3,761,040 - +0.8 3,761,040 - -2.0 3,761,068 + -0.2 3,761,068 + +1.5 3,761,069 - -0.2 3,761,298 + Pf1N1B4_3374 0.28 -0.0 3,761,444 + Pf1N1B4_3374 0.43 +0.3 3,761,444 + Pf1N1B4_3374 0.43 -1.7 3,761,445 - Pf1N1B4_3374 0.43 +1.9 3,761,445 - Pf1N1B4_3374 0.43 -2.2 3,761,445 - +1.1 3,761,544 + +1.6 3,761,544 + Pf1N1B4_3374 0.53 -1.4 3,761,544 + Pf1N1B4_3374 0.53 +1.7 3,761,545 - Pf1N1B4_3374 0.53 +1.3 3,761,595 - Pf1N1B4_3374 0.58 -0.7 3,761,596 + +1.1 3,761,597 - Pf1N1B4_3374 0.58 +0.6 3,761,843 - Pf1N1B4_3374 0.84 -1.2 3,761,843 - Pf1N1B4_3374 0.84 +1.0
Or see this region's nucleotide sequence