Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3301 and Pf1N1B4_3302 are separated by 305 nucleotides Pf1N1B4_3302 and Pf1N1B4_3303 are separated by 11 nucleotides Pf1N1B4_3303 and Pf1N1B4_3304 are separated by 12 nucleotides Pf1N1B4_3304 and Pf1N1B4_3305 overlap by 4 nucleotides
Pf1N1B4_3301 - Urea carboxylase (EC 6.3.4.6), at 3,687,408 to 3,691,049
_3301
Pf1N1B4_3302 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,691,355 to 3,691,996
_3302
Pf1N1B4_3303 - Urea carboxylase-related aminomethyltransferase (EC 2.1.2.10), at 3,692,008 to 3,692,733
_3303
Pf1N1B4_3304 - Urea carboxylase-related ABC transporter, ATPase protein, at 3,692,746 to 3,693,534
_3304
Pf1N1B4_3305 - Urea carboxylase-related ABC transporter, permease protein, at 3,693,531 to 3,694,346
_3305
Position (kb)
3692
3693 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.284 kb on - strand at 3691.392 kb on + strand at 3691.392 kb on + strand at 3691.393 kb on - strand at 3691.470 kb on + strand at 3691.470 kb on + strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.471 kb on - strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.605 kb on + strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.606 kb on - strand, within Pf1N1B4_3302 at 3691.629 kb on + strand, within Pf1N1B4_3302 at 3691.749 kb on + strand, within Pf1N1B4_3302 at 3691.750 kb on - strand, within Pf1N1B4_3302 at 3691.773 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.805 kb on + strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.806 kb on - strand, within Pf1N1B4_3302 at 3691.813 kb on - strand, within Pf1N1B4_3302 at 3691.840 kb on - strand, within Pf1N1B4_3302 at 3692.195 kb on + strand, within Pf1N1B4_3303 at 3692.207 kb on + strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand, within Pf1N1B4_3303 at 3692.208 kb on - strand at 3692.322 kb on + strand, within Pf1N1B4_3303 at 3692.747 kb on + strand at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.837 kb on + strand, within Pf1N1B4_3304 at 3692.838 kb on - strand at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.838 kb on - strand, within Pf1N1B4_3304 at 3692.855 kb on + strand at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3692.856 kb on - strand, within Pf1N1B4_3304 at 3693.370 kb on - strand, within Pf1N1B4_3304 at 3693.388 kb on + strand, within Pf1N1B4_3304 at 3693.389 kb on - strand, within Pf1N1B4_3304 at 3693.391 kb on + strand, within Pf1N1B4_3304 at 3693.392 kb on - strand at 3693.485 kb on - strand at 3693.497 kb on + strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.498 kb on - strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.550 kb on + strand at 3693.551 kb on - strand at 3693.704 kb on + strand, within Pf1N1B4_3305 at 3693.704 kb on + strand, within Pf1N1B4_3305
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,691,284 - -1.8 3,691,284 - +2.5 3,691,284 - -0.4 3,691,392 + +0.9 3,691,392 + +1.5 3,691,393 - -0.7 3,691,470 + +1.8 3,691,470 + Pf1N1B4_3302 0.18 +1.2 3,691,471 - Pf1N1B4_3302 0.18 +1.8 3,691,471 - Pf1N1B4_3302 0.18 +1.8 3,691,471 - Pf1N1B4_3302 0.18 -2.2 3,691,605 + Pf1N1B4_3302 0.39 +0.4 3,691,605 + Pf1N1B4_3302 0.39 -0.6 3,691,606 - Pf1N1B4_3302 0.39 -2.7 3,691,606 - Pf1N1B4_3302 0.39 -0.8 3,691,606 - Pf1N1B4_3302 0.39 +2.2 3,691,606 - Pf1N1B4_3302 0.39 +0.1 3,691,606 - -0.8 3,691,606 - Pf1N1B4_3302 0.39 -1.1 3,691,606 - Pf1N1B4_3302 0.39 +2.8 3,691,629 + Pf1N1B4_3302 0.43 -2.8 3,691,749 + Pf1N1B4_3302 0.61 +1.2 3,691,750 - Pf1N1B4_3302 0.62 +1.8 3,691,773 + Pf1N1B4_3302 0.65 -0.4 3,691,805 + Pf1N1B4_3302 0.70 -1.1 3,691,805 + Pf1N1B4_3302 0.70 -0.8 3,691,805 + +0.9 3,691,805 + Pf1N1B4_3302 0.70 +0.2 3,691,805 + Pf1N1B4_3302 0.70 +2.8 3,691,806 - Pf1N1B4_3302 0.70 +0.2 3,691,806 - Pf1N1B4_3302 0.70 -0.4 3,691,806 - Pf1N1B4_3302 0.70 -1.9 3,691,806 - Pf1N1B4_3302 0.70 -0.9 3,691,813 - Pf1N1B4_3302 0.71 -2.4 3,691,840 - Pf1N1B4_3302 0.76 +0.5 3,692,195 + Pf1N1B4_3303 0.26 -1.8 3,692,207 + Pf1N1B4_3303 0.27 +0.2 3,692,208 - Pf1N1B4_3303 0.28 -1.8 3,692,208 - Pf1N1B4_3303 0.28 -2.6 3,692,208 - +1.2 3,692,322 + Pf1N1B4_3303 0.43 -1.8 3,692,747 + +1.1 3,692,837 + Pf1N1B4_3304 0.12 +1.0 3,692,837 + Pf1N1B4_3304 0.12 -0.4 3,692,838 - +1.2 3,692,838 - Pf1N1B4_3304 0.12 +1.0 3,692,838 - Pf1N1B4_3304 0.12 -0.4 3,692,855 + -0.4 3,692,856 - Pf1N1B4_3304 0.14 -1.4 3,692,856 - Pf1N1B4_3304 0.14 -0.6 3,693,370 - Pf1N1B4_3304 0.79 -0.1 3,693,388 + Pf1N1B4_3304 0.81 -0.2 3,693,389 - Pf1N1B4_3304 0.81 +0.2 3,693,391 + Pf1N1B4_3304 0.82 +0.3 3,693,392 - -0.8 3,693,485 - +2.5 3,693,497 + -2.2 3,693,498 - +1.6 3,693,498 - +1.3 3,693,498 - +0.2 3,693,550 + +2.0 3,693,550 + +1.0 3,693,550 + +0.2 3,693,551 - +0.4 3,693,704 + Pf1N1B4_3305 0.21 +1.0 3,693,704 + Pf1N1B4_3305 0.21 -1.6
Or see this region's nucleotide sequence