Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3263 and Pf1N1B4_3264 are separated by 175 nucleotides Pf1N1B4_3264 and Pf1N1B4_3265 are separated by 59 nucleotides
Pf1N1B4_3263 - FIG126011: Hypothetical protein, at 3,653,163 to 3,654,572
_3263
Pf1N1B4_3264 - Methyltransferase (EC 2.1.1.-), at 3,654,748 to 3,655,962
_3264
Pf1N1B4_3265 - Permease of the drug/metabolite transporter (DMT) superfamily, at 3,656,022 to 3,656,942
_3265
Position (kb)
3654
3655
3656 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5
6
7 at 3653.912 kb on + strand, within Pf1N1B4_3263 at 3653.933 kb on + strand, within Pf1N1B4_3263 at 3653.962 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.072 kb on + strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.073 kb on - strand, within Pf1N1B4_3263 at 3654.129 kb on + strand, within Pf1N1B4_3263 at 3654.129 kb on + strand, within Pf1N1B4_3263 at 3654.130 kb on - strand, within Pf1N1B4_3263 at 3654.130 kb on - strand, within Pf1N1B4_3263 at 3654.357 kb on + strand, within Pf1N1B4_3263 at 3654.357 kb on + strand, within Pf1N1B4_3263 at 3654.358 kb on - strand, within Pf1N1B4_3263 at 3654.570 kb on + strand at 3654.669 kb on - strand at 3654.669 kb on - strand at 3654.699 kb on - strand at 3654.703 kb on + strand at 3654.862 kb on + strand at 3654.863 kb on - strand at 3654.949 kb on + strand, within Pf1N1B4_3264 at 3654.951 kb on + strand, within Pf1N1B4_3264 at 3654.951 kb on + strand, within Pf1N1B4_3264 at 3654.952 kb on - strand, within Pf1N1B4_3264 at 3655.043 kb on + strand, within Pf1N1B4_3264 at 3655.384 kb on + strand, within Pf1N1B4_3264 at 3655.384 kb on + strand at 3655.384 kb on + strand, within Pf1N1B4_3264 at 3655.385 kb on - strand, within Pf1N1B4_3264 at 3655.583 kb on - strand, within Pf1N1B4_3264 at 3655.906 kb on + strand at 3655.955 kb on - strand at 3656.020 kb on + strand at 3656.021 kb on - strand at 3656.021 kb on - strand at 3656.021 kb on - strand at 3656.023 kb on + strand at 3656.024 kb on - strand at 3656.139 kb on - strand, within Pf1N1B4_3265 at 3656.139 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.294 kb on - strand, within Pf1N1B4_3265 at 3656.351 kb on - strand, within Pf1N1B4_3265 at 3656.351 kb on - strand, within Pf1N1B4_3265 at 3656.530 kb on + strand, within Pf1N1B4_3265 at 3656.612 kb on - strand, within Pf1N1B4_3265 at 3656.612 kb on - strand, within Pf1N1B4_3265 at 3656.892 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,653,912 + Pf1N1B4_3263 0.53 -2.1 3,653,933 + Pf1N1B4_3263 0.55 +0.5 3,653,962 + Pf1N1B4_3263 0.57 +0.1 3,654,072 + Pf1N1B4_3263 0.64 -1.3 3,654,072 + Pf1N1B4_3263 0.64 -0.3 3,654,072 + Pf1N1B4_3263 0.64 -0.3 3,654,072 + Pf1N1B4_3263 0.64 +0.3 3,654,073 - Pf1N1B4_3263 0.65 +0.9 3,654,073 - Pf1N1B4_3263 0.65 +2.1 3,654,073 - Pf1N1B4_3263 0.65 -0.3 3,654,073 - Pf1N1B4_3263 0.65 -0.3 3,654,129 + Pf1N1B4_3263 0.69 +3.3 3,654,129 + Pf1N1B4_3263 0.69 -1.6 3,654,130 - Pf1N1B4_3263 0.69 +0.7 3,654,130 - Pf1N1B4_3263 0.69 -0.7 3,654,357 + Pf1N1B4_3263 0.85 +0.3 3,654,357 + Pf1N1B4_3263 0.85 -0.3 3,654,358 - Pf1N1B4_3263 0.85 +2.4 3,654,570 + +0.8 3,654,669 - +4.2 3,654,669 - -0.1 3,654,699 - +1.7 3,654,703 + -2.4 3,654,862 + +5.4 3,654,863 - +4.5 3,654,949 + Pf1N1B4_3264 0.17 +7.5 3,654,951 + Pf1N1B4_3264 0.17 +3.8 3,654,951 + Pf1N1B4_3264 0.17 +4.8 3,654,952 - Pf1N1B4_3264 0.17 +5.8 3,655,043 + Pf1N1B4_3264 0.24 +7.0 3,655,384 + Pf1N1B4_3264 0.52 +4.9 3,655,384 + +7.0 3,655,384 + Pf1N1B4_3264 0.52 +7.0 3,655,385 - Pf1N1B4_3264 0.52 +6.0 3,655,583 - Pf1N1B4_3264 0.69 +7.3 3,655,906 + +5.5 3,655,955 - +1.6 3,656,020 + -1.3 3,656,021 - -0.3 3,656,021 - +1.8 3,656,021 - -0.0 3,656,023 + -0.3 3,656,024 - -0.7 3,656,139 - Pf1N1B4_3265 0.13 -1.3 3,656,139 - Pf1N1B4_3265 0.13 +0.1 3,656,294 - Pf1N1B4_3265 0.30 +2.1 3,656,294 - Pf1N1B4_3265 0.30 +2.0 3,656,294 - Pf1N1B4_3265 0.30 -0.1 3,656,351 - Pf1N1B4_3265 0.36 +0.3 3,656,351 - Pf1N1B4_3265 0.36 -1.6 3,656,530 + Pf1N1B4_3265 0.55 -0.5 3,656,612 - Pf1N1B4_3265 0.64 +0.3 3,656,612 - Pf1N1B4_3265 0.64 -0.2 3,656,892 - +0.3
Or see this region's nucleotide sequence