Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3256 and Pf1N1B4_3257 are separated by 186 nucleotides Pf1N1B4_3257 and Pf1N1B4_3258 overlap by 4 nucleotides Pf1N1B4_3258 and Pf1N1B4_3259 are separated by 234 nucleotides
Pf1N1B4_3256 - Acyl-CoA dehydrogenase; probable dibenzothiophene desulfurization enzyme, at 3,645,265 to 3,646,146
_3256
Pf1N1B4_3257 - Beta-lactamase class C and other penicillin binding proteins, at 3,646,333 to 3,647,427
_3257
Pf1N1B4_3258 - FIG00953409: hypothetical protein, at 3,647,424 to 3,647,753
_3258
Pf1N1B4_3259 - FIG00956006: hypothetical protein, at 3,647,988 to 3,648,578
_3259
Position (kb)
3646
3647
3648 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3645.434 kb on - strand, within Pf1N1B4_3256 at 3645.725 kb on + strand, within Pf1N1B4_3256 at 3645.725 kb on + strand, within Pf1N1B4_3256 at 3645.725 kb on + strand, within Pf1N1B4_3256 at 3645.725 kb on + strand at 3645.726 kb on - strand, within Pf1N1B4_3256 at 3645.726 kb on - strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand, within Pf1N1B4_3256 at 3645.793 kb on + strand at 3645.794 kb on - strand, within Pf1N1B4_3256 at 3645.794 kb on - strand at 3645.794 kb on - strand, within Pf1N1B4_3256 at 3645.794 kb on - strand, within Pf1N1B4_3256 at 3645.794 kb on - strand, within Pf1N1B4_3256 at 3645.794 kb on - strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.826 kb on + strand, within Pf1N1B4_3256 at 3645.827 kb on - strand, within Pf1N1B4_3256 at 3645.827 kb on - strand at 3645.827 kb on - strand, within Pf1N1B4_3256 at 3645.827 kb on - strand, within Pf1N1B4_3256 at 3645.827 kb on - strand, within Pf1N1B4_3256 at 3646.205 kb on + strand at 3646.205 kb on + strand at 3646.206 kb on - strand at 3646.206 kb on - strand at 3646.212 kb on + strand at 3646.213 kb on - strand at 3646.289 kb on + strand at 3646.289 kb on + strand at 3646.289 kb on + strand at 3646.289 kb on + strand at 3646.290 kb on - strand at 3646.290 kb on - strand at 3646.298 kb on + strand at 3646.298 kb on + strand at 3646.299 kb on - strand at 3646.371 kb on + strand at 3646.463 kb on - strand at 3646.463 kb on - strand, within Pf1N1B4_3257 at 3646.463 kb on - strand, within Pf1N1B4_3257 at 3646.463 kb on - strand, within Pf1N1B4_3257 at 3646.546 kb on + strand, within Pf1N1B4_3257 at 3646.546 kb on + strand, within Pf1N1B4_3257 at 3646.547 kb on - strand at 3646.547 kb on - strand, within Pf1N1B4_3257 at 3646.547 kb on - strand, within Pf1N1B4_3257 at 3646.555 kb on + strand, within Pf1N1B4_3257 at 3646.765 kb on + strand at 3646.765 kb on + strand, within Pf1N1B4_3257 at 3646.765 kb on + strand, within Pf1N1B4_3257 at 3646.771 kb on + strand, within Pf1N1B4_3257 at 3646.771 kb on + strand, within Pf1N1B4_3257 at 3646.772 kb on - strand, within Pf1N1B4_3257 at 3646.772 kb on - strand, within Pf1N1B4_3257 at 3646.807 kb on + strand at 3646.808 kb on - strand, within Pf1N1B4_3257 at 3646.808 kb on - strand, within Pf1N1B4_3257 at 3646.808 kb on - strand, within Pf1N1B4_3257 at 3646.808 kb on - strand, within Pf1N1B4_3257 at 3646.858 kb on + strand, within Pf1N1B4_3257 at 3647.044 kb on + strand, within Pf1N1B4_3257 at 3647.045 kb on - strand, within Pf1N1B4_3257 at 3647.045 kb on - strand, within Pf1N1B4_3257 at 3647.045 kb on - strand, within Pf1N1B4_3257 at 3647.050 kb on + strand, within Pf1N1B4_3257 at 3647.051 kb on - strand at 3647.051 kb on - strand, within Pf1N1B4_3257 at 3647.051 kb on - strand, within Pf1N1B4_3257 at 3647.305 kb on + strand at 3647.305 kb on + strand, within Pf1N1B4_3257 at 3647.306 kb on - strand, within Pf1N1B4_3257 at 3647.306 kb on - strand, within Pf1N1B4_3257 at 3647.384 kb on + strand at 3647.384 kb on + strand at 3647.385 kb on - strand at 3647.385 kb on - strand at 3647.441 kb on + strand at 3647.554 kb on - strand, within Pf1N1B4_3258 at 3647.554 kb on - strand, within Pf1N1B4_3258 at 3647.568 kb on + strand, within Pf1N1B4_3258 at 3647.568 kb on + strand, within Pf1N1B4_3258 at 3647.568 kb on + strand, within Pf1N1B4_3258 at 3647.569 kb on - strand, within Pf1N1B4_3258 at 3647.569 kb on - strand, within Pf1N1B4_3258 at 3647.583 kb on + strand, within Pf1N1B4_3258 at 3647.584 kb on - strand, within Pf1N1B4_3258 at 3647.619 kb on + strand, within Pf1N1B4_3258 at 3647.620 kb on - strand, within Pf1N1B4_3258 at 3647.631 kb on + strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.632 kb on - strand, within Pf1N1B4_3258 at 3647.699 kb on + strand, within Pf1N1B4_3258 at 3647.700 kb on - strand, within Pf1N1B4_3258 at 3647.813 kb on + strand at 3647.835 kb on - strand at 3647.970 kb on - strand at 3647.970 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3647.988 kb on - strand at 3648.108 kb on - strand, within Pf1N1B4_3259 at 3648.119 kb on + strand, within Pf1N1B4_3259 at 3648.302 kb on - strand, within Pf1N1B4_3259 at 3648.302 kb on - strand at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.387 kb on + strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand, within Pf1N1B4_3259 at 3648.388 kb on - strand at 3648.388 kb on - strand, within Pf1N1B4_3259
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,645,434 - Pf1N1B4_3256 0.19 -1.7 3,645,725 + Pf1N1B4_3256 0.52 +0.5 3,645,725 + Pf1N1B4_3256 0.52 +0.5 3,645,725 + Pf1N1B4_3256 0.52 +1.0 3,645,725 + +0.3 3,645,726 - Pf1N1B4_3256 0.52 -1.0 3,645,726 - Pf1N1B4_3256 0.52 +0.4 3,645,793 + Pf1N1B4_3256 0.60 +0.4 3,645,793 + Pf1N1B4_3256 0.60 +0.4 3,645,793 + Pf1N1B4_3256 0.60 +0.3 3,645,793 + Pf1N1B4_3256 0.60 +2.4 3,645,793 + Pf1N1B4_3256 0.60 +1.3 3,645,793 + Pf1N1B4_3256 0.60 +2.1 3,645,793 + -0.7 3,645,794 - Pf1N1B4_3256 0.60 +0.3 3,645,794 - +2.3 3,645,794 - Pf1N1B4_3256 0.60 -0.6 3,645,794 - Pf1N1B4_3256 0.60 -0.4 3,645,794 - Pf1N1B4_3256 0.60 -0.0 3,645,794 - Pf1N1B4_3256 0.60 -0.0 3,645,826 + Pf1N1B4_3256 0.64 +0.5 3,645,826 + Pf1N1B4_3256 0.64 -0.7 3,645,826 + Pf1N1B4_3256 0.64 -0.9 3,645,826 + Pf1N1B4_3256 0.64 -0.1 3,645,826 + Pf1N1B4_3256 0.64 -1.0 3,645,826 + Pf1N1B4_3256 0.64 -0.0 3,645,826 + Pf1N1B4_3256 0.64 -1.0 3,645,826 + Pf1N1B4_3256 0.64 -0.8 3,645,826 + Pf1N1B4_3256 0.64 +0.1 3,645,826 + Pf1N1B4_3256 0.64 -1.5 3,645,826 + Pf1N1B4_3256 0.64 -1.2 3,645,826 + Pf1N1B4_3256 0.64 -0.2 3,645,826 + Pf1N1B4_3256 0.64 +0.5 3,645,826 + Pf1N1B4_3256 0.64 -2.2 3,645,826 + Pf1N1B4_3256 0.64 +1.1 3,645,826 + Pf1N1B4_3256 0.64 -0.0 3,645,826 + Pf1N1B4_3256 0.64 +1.3 3,645,826 + Pf1N1B4_3256 0.64 -1.0 3,645,826 + Pf1N1B4_3256 0.64 -1.1 3,645,826 + Pf1N1B4_3256 0.64 -1.0 3,645,826 + Pf1N1B4_3256 0.64 +0.4 3,645,826 + Pf1N1B4_3256 0.64 +1.5 3,645,826 + Pf1N1B4_3256 0.64 -0.3 3,645,827 - Pf1N1B4_3256 0.64 +1.7 3,645,827 - +0.3 3,645,827 - Pf1N1B4_3256 0.64 -0.7 3,645,827 - Pf1N1B4_3256 0.64 +1.6 3,645,827 - Pf1N1B4_3256 0.64 -2.0 3,646,205 + -2.4 3,646,205 + -0.3 3,646,206 - +2.0 3,646,206 - -0.3 3,646,212 + -0.3 3,646,213 - -2.4 3,646,289 + +1.3 3,646,289 + +0.5 3,646,289 + +0.7 3,646,289 + -1.3 3,646,290 - +0.9 3,646,290 - +0.8 3,646,298 + +1.6 3,646,298 + +0.5 3,646,299 - +0.5 3,646,371 + -0.7 3,646,463 - -0.7 3,646,463 - Pf1N1B4_3257 0.12 +2.1 3,646,463 - Pf1N1B4_3257 0.12 +0.1 3,646,463 - Pf1N1B4_3257 0.12 -0.7 3,646,546 + Pf1N1B4_3257 0.19 -1.6 3,646,546 + Pf1N1B4_3257 0.19 +1.7 3,646,547 - +1.3 3,646,547 - Pf1N1B4_3257 0.20 -1.2 3,646,547 - Pf1N1B4_3257 0.20 -1.3 3,646,555 + Pf1N1B4_3257 0.20 +0.7 3,646,765 + +1.7 3,646,765 + Pf1N1B4_3257 0.39 +0.8 3,646,765 + Pf1N1B4_3257 0.39 +0.2 3,646,771 + Pf1N1B4_3257 0.40 +0.6 3,646,771 + Pf1N1B4_3257 0.40 -1.0 3,646,772 - Pf1N1B4_3257 0.40 -0.5 3,646,772 - Pf1N1B4_3257 0.40 -0.3 3,646,807 + +0.3 3,646,808 - Pf1N1B4_3257 0.43 -0.9 3,646,808 - Pf1N1B4_3257 0.43 +1.3 3,646,808 - Pf1N1B4_3257 0.43 +2.9 3,646,808 - Pf1N1B4_3257 0.43 +0.1 3,646,858 + Pf1N1B4_3257 0.48 +1.1 3,647,044 + Pf1N1B4_3257 0.65 +0.6 3,647,045 - Pf1N1B4_3257 0.65 -1.3 3,647,045 - Pf1N1B4_3257 0.65 +0.7 3,647,045 - Pf1N1B4_3257 0.65 +1.5 3,647,050 + Pf1N1B4_3257 0.65 +0.3 3,647,051 - -0.3 3,647,051 - Pf1N1B4_3257 0.66 +1.3 3,647,051 - Pf1N1B4_3257 0.66 -0.5 3,647,305 + +1.9 3,647,305 + Pf1N1B4_3257 0.89 -0.9 3,647,306 - Pf1N1B4_3257 0.89 +1.0 3,647,306 - Pf1N1B4_3257 0.89 -0.3 3,647,384 + -1.7 3,647,384 + -0.0 3,647,385 - -0.3 3,647,385 - -0.7 3,647,441 + -1.9 3,647,554 - Pf1N1B4_3258 0.39 -2.0 3,647,554 - Pf1N1B4_3258 0.39 +1.3 3,647,568 + Pf1N1B4_3258 0.44 -1.5 3,647,568 + Pf1N1B4_3258 0.44 -0.3 3,647,568 + Pf1N1B4_3258 0.44 +1.3 3,647,569 - Pf1N1B4_3258 0.44 -0.5 3,647,569 - Pf1N1B4_3258 0.44 +1.7 3,647,583 + Pf1N1B4_3258 0.48 +1.3 3,647,584 - Pf1N1B4_3258 0.48 +0.1 3,647,619 + Pf1N1B4_3258 0.59 -1.5 3,647,620 - Pf1N1B4_3258 0.59 +2.3 3,647,631 + Pf1N1B4_3258 0.63 +0.1 3,647,632 - Pf1N1B4_3258 0.63 -0.5 3,647,632 - Pf1N1B4_3258 0.63 -0.0 3,647,632 - Pf1N1B4_3258 0.63 +2.6 3,647,632 - Pf1N1B4_3258 0.63 -2.5 3,647,699 + Pf1N1B4_3258 0.83 +2.6 3,647,700 - Pf1N1B4_3258 0.84 +0.4 3,647,813 + +1.3 3,647,835 - +0.0 3,647,970 - +0.0 3,647,970 - +3.3 3,647,988 - -1.2 3,647,988 - +1.4 3,647,988 - -1.1 3,647,988 - +1.9 3,648,108 - Pf1N1B4_3259 0.20 +1.4 3,648,119 + Pf1N1B4_3259 0.22 -0.7 3,648,302 - Pf1N1B4_3259 0.53 -0.5 3,648,302 - -0.3 3,648,387 + Pf1N1B4_3259 0.68 -1.1 3,648,387 + Pf1N1B4_3259 0.68 +1.1 3,648,387 + Pf1N1B4_3259 0.68 -1.3 3,648,387 + Pf1N1B4_3259 0.68 +0.0 3,648,387 + Pf1N1B4_3259 0.68 +0.5 3,648,388 - Pf1N1B4_3259 0.68 -1.3 3,648,388 - Pf1N1B4_3259 0.68 -0.1 3,648,388 - Pf1N1B4_3259 0.68 +0.9 3,648,388 - Pf1N1B4_3259 0.68 +0.3 3,648,388 - +0.3 3,648,388 - Pf1N1B4_3259 0.68 +0.9
Or see this region's nucleotide sequence