Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3232 and Pf1N1B4_3233 overlap by 1 nucleotides Pf1N1B4_3233 and Pf1N1B4_3234 overlap by 1 nucleotides Pf1N1B4_3234 and Pf1N1B4_3235 are separated by 51 nucleotides Pf1N1B4_3235 and Pf1N1B4_3236 are separated by 1 nucleotides
Pf1N1B4_3232 - 3-oxoadipate CoA-transferase subunit A (EC 2.8.3.6); Glutaconate CoA-transferase subunit A (EC 2.8.3.12), at 3,619,249 to 3,620,106
_3232
Pf1N1B4_3233 - 3-oxoadipate CoA-transferase subunit B (EC 2.8.3.6); Glutaconate CoA-transferase subunit B (EC 2.8.3.12), at 3,620,106 to 3,620,885
_3233
Pf1N1B4_3234 - Beta-ketoadipyl CoA thiolase (EC 2.3.1.-), at 3,620,885 to 3,622,087
_3234
Pf1N1B4_3235 - Protocatechuate 3,4-dioxygenase beta chain (EC 1.13.11.3), at 3,622,139 to 3,622,843
_3235
Pf1N1B4_3236 - Protocatechuate 3,4-dioxygenase alpha chain (EC 1.13.11.3), at 3,622,845 to 3,623,411
_3236
Position (kb)
3620
3621
3622
3623 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3619.920 kb on - strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand, within Pf1N1B4_3232 at 3619.978 kb on + strand at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3619.979 kb on - strand, within Pf1N1B4_3232 at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.038 kb on + strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.039 kb on - strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.104 kb on + strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.105 kb on - strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.112 kb on + strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.113 kb on - strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.239 kb on + strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.240 kb on - strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.250 kb on + strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.251 kb on - strand, within Pf1N1B4_3233 at 3620.284 kb on + strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.455 kb on - strand, within Pf1N1B4_3233 at 3620.524 kb on + strand, within Pf1N1B4_3233 at 3620.525 kb on - strand, within Pf1N1B4_3233 at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.897 kb on + strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.898 kb on - strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.899 kb on + strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3620.900 kb on - strand at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.084 kb on + strand, within Pf1N1B4_3234 at 3621.085 kb on - strand, within Pf1N1B4_3234 at 3621.726 kb on + strand at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.726 kb on + strand, within Pf1N1B4_3234 at 3621.727 kb on - strand, within Pf1N1B4_3234 at 3621.982 kb on - strand at 3622.104 kb on + strand at 3622.105 kb on - strand at 3622.116 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.119 kb on + strand at 3622.120 kb on - strand at 3622.138 kb on - strand at 3622.138 kb on - strand at 3622.190 kb on + strand at 3622.190 kb on + strand at 3622.191 kb on - strand at 3622.206 kb on - strand at 3622.365 kb on + strand at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.365 kb on + strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.366 kb on - strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.664 kb on + strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.665 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.692 kb on - strand, within Pf1N1B4_3235 at 3622.703 kb on + strand, within Pf1N1B4_3235 at 3622.778 kb on + strand at 3622.779 kb on - strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.841 kb on + strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.842 kb on - strand at 3622.884 kb on + strand at 3622.885 kb on - strand at 3622.885 kb on - strand at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.043 kb on + strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236 at 3623.044 kb on - strand, within Pf1N1B4_3236
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,619,920 - Pf1N1B4_3232 0.78 +0.3 3,619,978 + Pf1N1B4_3232 0.85 -0.3 3,619,978 + Pf1N1B4_3232 0.85 +1.3 3,619,978 + -0.3 3,619,979 - Pf1N1B4_3232 0.85 +0.3 3,619,979 - Pf1N1B4_3232 0.85 -1.9 3,620,038 + +1.9 3,620,038 + -1.1 3,620,038 + -0.2 3,620,038 + +0.3 3,620,038 + -1.9 3,620,038 + +1.0 3,620,038 + -2.6 3,620,039 - +0.3 3,620,039 - -0.0 3,620,039 - +1.3 3,620,039 - -1.2 3,620,104 + -0.5 3,620,104 + -0.7 3,620,104 + -1.3 3,620,105 - -0.3 3,620,105 - -1.2 3,620,105 - +0.1 3,620,112 + +0.4 3,620,112 + -0.7 3,620,112 + -1.1 3,620,113 - +2.7 3,620,113 - -0.1 3,620,113 - +0.9 3,620,113 - -0.3 3,620,113 - -1.3 3,620,113 - +2.3 3,620,113 - +1.3 3,620,239 + Pf1N1B4_3233 0.17 +0.3 3,620,239 + +0.3 3,620,239 + Pf1N1B4_3233 0.17 +0.5 3,620,239 + Pf1N1B4_3233 0.17 +1.7 3,620,240 - Pf1N1B4_3233 0.17 -0.2 3,620,240 - Pf1N1B4_3233 0.17 +0.0 3,620,240 - Pf1N1B4_3233 0.17 -1.1 3,620,250 + Pf1N1B4_3233 0.18 -1.1 3,620,250 + Pf1N1B4_3233 0.18 -1.7 3,620,250 + Pf1N1B4_3233 0.18 -0.1 3,620,250 + Pf1N1B4_3233 0.18 -0.3 3,620,250 + Pf1N1B4_3233 0.18 +0.3 3,620,251 - Pf1N1B4_3233 0.19 +0.9 3,620,251 - Pf1N1B4_3233 0.19 -0.6 3,620,251 - Pf1N1B4_3233 0.19 +3.4 3,620,284 + Pf1N1B4_3233 0.23 +0.0 3,620,455 - Pf1N1B4_3233 0.45 +1.9 3,620,455 - Pf1N1B4_3233 0.45 +0.8 3,620,524 + Pf1N1B4_3233 0.54 -0.1 3,620,525 - Pf1N1B4_3233 0.54 +1.3 3,620,897 + -0.5 3,620,897 + -1.8 3,620,897 + +1.3 3,620,897 + +1.0 3,620,897 + +0.6 3,620,897 + +1.3 3,620,897 + +0.8 3,620,897 + +2.3 3,620,898 - +1.4 3,620,898 - -0.1 3,620,898 - -0.1 3,620,898 - -0.7 3,620,899 + -0.2 3,620,899 + +1.3 3,620,899 + +0.8 3,620,899 + +0.2 3,620,899 + -2.2 3,620,899 + -0.5 3,620,900 - +1.4 3,620,900 - -0.7 3,620,900 - -2.5 3,621,084 + Pf1N1B4_3234 0.17 +0.7 3,621,084 + Pf1N1B4_3234 0.17 -1.7 3,621,084 + Pf1N1B4_3234 0.17 -1.7 3,621,085 - Pf1N1B4_3234 0.17 -0.3 3,621,726 + +3.3 3,621,726 + Pf1N1B4_3234 0.70 -2.3 3,621,726 + Pf1N1B4_3234 0.70 -0.3 3,621,726 + Pf1N1B4_3234 0.70 -1.5 3,621,727 - Pf1N1B4_3234 0.70 -1.7 3,621,982 - +0.3 3,622,104 + -2.6 3,622,105 - -2.2 3,622,116 + -0.5 3,622,119 + -0.7 3,622,119 + +1.3 3,622,119 + -1.9 3,622,120 - -0.3 3,622,138 - -0.7 3,622,138 - -0.3 3,622,190 + +0.3 3,622,190 + -1.5 3,622,191 - +0.7 3,622,206 - +0.3 3,622,365 + +1.9 3,622,365 + Pf1N1B4_3235 0.32 -2.2 3,622,365 + Pf1N1B4_3235 0.32 +0.6 3,622,366 - Pf1N1B4_3235 0.32 -1.0 3,622,366 - Pf1N1B4_3235 0.32 +0.5 3,622,366 - Pf1N1B4_3235 0.32 +0.7 3,622,664 + Pf1N1B4_3235 0.74 -0.3 3,622,664 + Pf1N1B4_3235 0.74 -0.5 3,622,664 + Pf1N1B4_3235 0.74 -1.3 3,622,664 + Pf1N1B4_3235 0.74 +0.3 3,622,665 - Pf1N1B4_3235 0.75 -0.9 3,622,665 - Pf1N1B4_3235 0.75 -1.7 3,622,665 - Pf1N1B4_3235 0.75 -0.2 3,622,665 - Pf1N1B4_3235 0.75 +0.7 3,622,665 - Pf1N1B4_3235 0.75 +0.1 3,622,665 - Pf1N1B4_3235 0.75 -1.0 3,622,692 - Pf1N1B4_3235 0.78 +0.5 3,622,692 - Pf1N1B4_3235 0.78 -1.3 3,622,703 + Pf1N1B4_3235 0.80 -1.3 3,622,778 + +0.4 3,622,779 - -0.2 3,622,841 + -1.8 3,622,841 + -0.5 3,622,841 + +2.9 3,622,841 + -1.1 3,622,841 + -0.3 3,622,841 + -0.7 3,622,842 - -1.2 3,622,842 - -1.7 3,622,842 - -0.4 3,622,884 + -3.0 3,622,885 - +1.3 3,622,885 - -0.7 3,623,043 + Pf1N1B4_3236 0.35 -1.5 3,623,043 + Pf1N1B4_3236 0.35 +1.0 3,623,043 + Pf1N1B4_3236 0.35 +0.3 3,623,043 + Pf1N1B4_3236 0.35 +1.3 3,623,044 - Pf1N1B4_3236 0.35 -0.7 3,623,044 - Pf1N1B4_3236 0.35 -1.3 3,623,044 - Pf1N1B4_3236 0.35 -0.7
Or see this region's nucleotide sequence