Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_31 and Pf1N1B4_32 are separated by 179 nucleotides Pf1N1B4_32 and Pf1N1B4_33 are separated by 174 nucleotides
Pf1N1B4_31 - Probable Co/Zn/Cd efflux system membrane fusion protein, at 35,896 to 37,017
_31
Pf1N1B4_32 - Transcriptional regulator, TetR family, at 37,197 to 37,817
_32
Pf1N1B4_33 - Outer membrane component of tripartite multidrug resistance system, at 37,992 to 39,422
_33
Position (kb) 37
38 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 36.245 kb on + strand at 36.245 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.370 kb on + strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.479 kb on - strand, within Pf1N1B4_31 at 36.560 kb on - strand, within Pf1N1B4_31 at 36.750 kb on + strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 36.751 kb on - strand, within Pf1N1B4_31 at 37.017 kb on + strand at 37.267 kb on + strand at 37.465 kb on + strand, within Pf1N1B4_32 at 37.482 kb on - strand at 37.558 kb on - strand, within Pf1N1B4_32 at 37.608 kb on - strand at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.657 kb on + strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand, within Pf1N1B4_32 at 37.658 kb on - strand at 37.687 kb on - strand, within Pf1N1B4_32 at 37.933 kb on + strand at 37.934 kb on - strand at 38.084 kb on + strand at 38.141 kb on - strand, within Pf1N1B4_33 at 38.295 kb on + strand, within Pf1N1B4_33 at 38.316 kb on + strand, within Pf1N1B4_33 at 38.317 kb on - strand, within Pf1N1B4_33 at 38.317 kb on - strand, within Pf1N1B4_33 at 38.463 kb on + strand at 38.463 kb on + strand, within Pf1N1B4_33 at 38.464 kb on - strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.515 kb on + strand, within Pf1N1B4_33 at 38.516 kb on - strand, within Pf1N1B4_33 at 38.516 kb on - strand, within Pf1N1B4_33 at 38.703 kb on + strand at 38.703 kb on + strand at 38.703 kb on + strand, within Pf1N1B4_33 at 38.703 kb on + strand, within Pf1N1B4_33 at 38.704 kb on - strand at 38.704 kb on - strand at 38.704 kb on - strand, within Pf1N1B4_33 at 38.706 kb on - strand, within Pf1N1B4_33 at 38.721 kb on - strand, within Pf1N1B4_33
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 36,245 + -0.9 36,245 + Pf1N1B4_31 0.31 +1.8 36,370 + Pf1N1B4_31 0.42 -1.7 36,370 + Pf1N1B4_31 0.42 +0.1 36,479 - Pf1N1B4_31 0.52 +0.3 36,479 - Pf1N1B4_31 0.52 -0.9 36,479 - Pf1N1B4_31 0.52 +0.1 36,479 - Pf1N1B4_31 0.52 +1.1 36,560 - Pf1N1B4_31 0.59 +1.3 36,750 + Pf1N1B4_31 0.76 +0.8 36,751 - Pf1N1B4_31 0.76 +0.5 36,751 - Pf1N1B4_31 0.76 +1.0 37,017 + +0.2 37,267 + -0.9 37,465 + Pf1N1B4_32 0.43 -0.5 37,482 - -0.9 37,558 - Pf1N1B4_32 0.58 -0.2 37,608 - +0.5 37,657 + Pf1N1B4_32 0.74 -1.7 37,657 + Pf1N1B4_32 0.74 -2.1 37,657 + Pf1N1B4_32 0.74 +2.1 37,658 - Pf1N1B4_32 0.74 +0.1 37,658 - -0.9 37,658 - +0.1 37,658 - -0.5 37,658 - Pf1N1B4_32 0.74 -1.4 37,658 - Pf1N1B4_32 0.74 +0.1 37,658 - Pf1N1B4_32 0.74 -1.4 37,658 - -0.5 37,687 - Pf1N1B4_32 0.79 -1.2 37,933 + -0.7 37,934 - -1.2 38,084 + +0.8 38,141 - Pf1N1B4_33 0.10 +0.2 38,295 + Pf1N1B4_33 0.21 +0.3 38,316 + Pf1N1B4_33 0.23 +0.4 38,317 - Pf1N1B4_33 0.23 +0.8 38,317 - Pf1N1B4_33 0.23 +0.1 38,463 + -0.9 38,463 + Pf1N1B4_33 0.33 -1.9 38,464 - Pf1N1B4_33 0.33 +0.7 38,515 + Pf1N1B4_33 0.37 -1.2 38,515 + Pf1N1B4_33 0.37 -0.4 38,515 + Pf1N1B4_33 0.37 -3.2 38,515 + Pf1N1B4_33 0.37 +0.5 38,516 - Pf1N1B4_33 0.37 +0.6 38,516 - Pf1N1B4_33 0.37 -0.5 38,703 + +1.8 38,703 + +3.7 38,703 + Pf1N1B4_33 0.50 +2.3 38,703 + Pf1N1B4_33 0.50 +1.5 38,704 - -0.9 38,704 - +0.1 38,704 - Pf1N1B4_33 0.50 -2.6 38,706 - Pf1N1B4_33 0.50 +0.1 38,721 - Pf1N1B4_33 0.51 -0.3
Or see this region's nucleotide sequence