Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_3146 and Pf1N1B4_3147 are separated by 0 nucleotides Pf1N1B4_3147 and Pf1N1B4_3148 are separated by 189 nucleotides Pf1N1B4_3148 and Pf1N1B4_3149 are separated by 87 nucleotides Pf1N1B4_3149 and Pf1N1B4_3150 are separated by 23 nucleotides Pf1N1B4_3150 and Pf1N1B4_3151 are separated by 75 nucleotides
Pf1N1B4_3146 - FIG00957354: hypothetical protein, at 3,528,929 to 3,529,123
_3146
Pf1N1B4_3147 - Putative NADPH-quinone reductase (modulator of drug activity B), at 3,529,124 to 3,529,549
_3147
Pf1N1B4_3148 - FIG074102: hypothetical protein, at 3,529,739 to 3,530,482
_3148
Pf1N1B4_3149 - hypothetical protein, at 3,530,570 to 3,530,698
_3149
Pf1N1B4_3150 - Transcriptional regulator, LuxR family, at 3,530,722 to 3,531,372
_3150
Pf1N1B4_3151 - Diacylglycerol kinase (EC 2.7.1.107), at 3,531,448 to 3,531,810
_3151
Position (kb)
3529
3530
3531 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3528.743 kb on + strand at 3528.744 kb on - strand at 3528.744 kb on - strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.791 kb on + strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.792 kb on - strand at 3528.810 kb on + strand at 3528.813 kb on + strand at 3528.813 kb on + strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.814 kb on - strand at 3528.818 kb on + strand at 3528.819 kb on - strand at 3528.819 kb on - strand at 3528.965 kb on + strand, within Pf1N1B4_3146 at 3529.058 kb on + strand, within Pf1N1B4_3146 at 3529.126 kb on - strand at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.293 kb on + strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.294 kb on - strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.378 kb on + strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.379 kb on - strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.380 kb on + strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.381 kb on - strand, within Pf1N1B4_3147 at 3529.436 kb on + strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.437 kb on - strand, within Pf1N1B4_3147 at 3529.538 kb on - strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.618 kb on + strand at 3529.619 kb on - strand at 3529.620 kb on + strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.621 kb on - strand at 3529.681 kb on + strand at 3529.681 kb on + strand at 3529.869 kb on + strand, within Pf1N1B4_3148 at 3529.870 kb on - strand, within Pf1N1B4_3148 at 3529.927 kb on + strand, within Pf1N1B4_3148 at 3530.063 kb on + strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.064 kb on - strand, within Pf1N1B4_3148 at 3530.084 kb on + strand at 3530.105 kb on - strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.360 kb on + strand, within Pf1N1B4_3148 at 3530.361 kb on - strand, within Pf1N1B4_3148 at 3530.384 kb on + strand, within Pf1N1B4_3148 at 3530.385 kb on - strand, within Pf1N1B4_3148 at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.454 kb on + strand at 3530.493 kb on + strand at 3530.610 kb on + strand, within Pf1N1B4_3149 at 3530.611 kb on - strand, within Pf1N1B4_3149 at 3530.611 kb on - strand, within Pf1N1B4_3149 at 3530.731 kb on + strand at 3530.731 kb on + strand at 3530.731 kb on + strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.732 kb on - strand at 3530.885 kb on + strand, within Pf1N1B4_3150 at 3530.886 kb on - strand, within Pf1N1B4_3150 at 3531.244 kb on + strand, within Pf1N1B4_3150 at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.370 kb on + strand at 3531.379 kb on - strand at 3531.411 kb on + strand at 3531.411 kb on + strand at 3531.411 kb on + strand at 3531.443 kb on - strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.447 kb on + strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand at 3531.448 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,528,743 + -0.5 3,528,744 - -1.9 3,528,744 - +0.4 3,528,791 + -2.1 3,528,791 + +1.9 3,528,791 + -1.1 3,528,792 - -0.2 3,528,792 - -1.1 3,528,792 - -2.1 3,528,792 - +0.4 3,528,810 + -0.3 3,528,813 + -0.3 3,528,813 + -2.1 3,528,814 - +1.8 3,528,814 - +0.2 3,528,814 - +1.6 3,528,814 - -0.8 3,528,814 - -0.9 3,528,818 + -2.1 3,528,819 - -0.3 3,528,819 - +0.2 3,528,965 + Pf1N1B4_3146 0.18 -1.6 3,529,058 + Pf1N1B4_3146 0.66 -3.2 3,529,126 - -2.3 3,529,293 + Pf1N1B4_3147 0.40 -1.6 3,529,293 + Pf1N1B4_3147 0.40 -1.3 3,529,294 - Pf1N1B4_3147 0.40 -2.8 3,529,294 - Pf1N1B4_3147 0.40 -3.3 3,529,294 - Pf1N1B4_3147 0.40 -1.3 3,529,378 + Pf1N1B4_3147 0.60 -0.2 3,529,378 + Pf1N1B4_3147 0.60 -2.2 3,529,378 + Pf1N1B4_3147 0.60 -0.6 3,529,378 + Pf1N1B4_3147 0.60 -0.8 3,529,378 + Pf1N1B4_3147 0.60 -1.0 3,529,378 + Pf1N1B4_3147 0.60 -1.9 3,529,378 + Pf1N1B4_3147 0.60 -1.8 3,529,379 - Pf1N1B4_3147 0.60 -0.2 3,529,379 - Pf1N1B4_3147 0.60 -3.7 3,529,379 - Pf1N1B4_3147 0.60 -0.6 3,529,379 - Pf1N1B4_3147 0.60 -1.8 3,529,379 - Pf1N1B4_3147 0.60 -1.8 3,529,379 - Pf1N1B4_3147 0.60 -1.3 3,529,379 - Pf1N1B4_3147 0.60 -1.1 3,529,379 - Pf1N1B4_3147 0.60 -1.0 3,529,379 - Pf1N1B4_3147 0.60 -2.7 3,529,380 + Pf1N1B4_3147 0.60 +0.8 3,529,380 + Pf1N1B4_3147 0.60 -3.7 3,529,380 + Pf1N1B4_3147 0.60 +0.5 3,529,381 - Pf1N1B4_3147 0.60 -1.8 3,529,381 - Pf1N1B4_3147 0.60 -1.6 3,529,381 - Pf1N1B4_3147 0.60 -0.8 3,529,381 - Pf1N1B4_3147 0.60 -2.1 3,529,436 + Pf1N1B4_3147 0.73 -2.0 3,529,437 - Pf1N1B4_3147 0.73 -0.6 3,529,437 - Pf1N1B4_3147 0.73 -2.3 3,529,437 - Pf1N1B4_3147 0.73 -0.8 3,529,437 - Pf1N1B4_3147 0.73 -1.4 3,529,437 - Pf1N1B4_3147 0.73 -1.3 3,529,538 - -2.7 3,529,618 + -0.3 3,529,618 + -1.1 3,529,618 + -1.8 3,529,618 + -1.8 3,529,618 + -1.3 3,529,618 + -1.6 3,529,619 - -0.3 3,529,620 + +0.2 3,529,621 - +0.7 3,529,621 - -0.2 3,529,621 - -0.5 3,529,621 - -0.6 3,529,681 + +0.3 3,529,681 + +0.2 3,529,869 + Pf1N1B4_3148 0.17 -0.3 3,529,870 - Pf1N1B4_3148 0.18 +1.4 3,529,927 + Pf1N1B4_3148 0.25 +0.9 3,530,063 + Pf1N1B4_3148 0.44 -1.6 3,530,064 - Pf1N1B4_3148 0.44 -0.1 3,530,064 - Pf1N1B4_3148 0.44 +1.5 3,530,084 + +0.2 3,530,105 - Pf1N1B4_3148 0.49 -0.3 3,530,360 + Pf1N1B4_3148 0.83 -1.9 3,530,360 + Pf1N1B4_3148 0.83 +1.6 3,530,360 + Pf1N1B4_3148 0.83 -0.8 3,530,361 - Pf1N1B4_3148 0.84 -0.3 3,530,384 + Pf1N1B4_3148 0.87 -0.1 3,530,385 - Pf1N1B4_3148 0.87 +0.6 3,530,454 + -0.3 3,530,454 + -0.1 3,530,454 + -1.1 3,530,454 + +0.2 3,530,454 + -1.1 3,530,493 + +1.0 3,530,610 + Pf1N1B4_3149 0.31 -2.3 3,530,611 - Pf1N1B4_3149 0.32 -0.3 3,530,611 - Pf1N1B4_3149 0.32 -1.3 3,530,731 + -0.8 3,530,731 + -0.2 3,530,731 + -0.8 3,530,732 - +0.6 3,530,732 - -0.3 3,530,732 - -0.6 3,530,732 - -0.1 3,530,885 + Pf1N1B4_3150 0.25 +1.7 3,530,886 - Pf1N1B4_3150 0.25 -0.3 3,531,244 + Pf1N1B4_3150 0.80 -0.1 3,531,370 + -0.5 3,531,370 + +2.7 3,531,370 + +2.1 3,531,370 + -0.8 3,531,370 + -0.1 3,531,370 + -1.3 3,531,379 - +1.6 3,531,411 + -0.9 3,531,411 + +1.9 3,531,411 + +1.4 3,531,443 - -0.3 3,531,447 + +1.2 3,531,447 + -0.3 3,531,447 + +1.4 3,531,447 + -0.2 3,531,448 - -0.3 3,531,448 - -0.1 3,531,448 - -1.6 3,531,448 - +0.8 3,531,448 - +3.1 3,531,448 - +0.5
Or see this region's nucleotide sequence