Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_312 and Pf1N1B4_313 are separated by 151 nucleotides Pf1N1B4_313 and Pf1N1B4_314 are separated by 200 nucleotides
Pf1N1B4_312 - Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-), at 365,145 to 367,796
_312
Pf1N1B4_313 - Potassium-transporting ATPase C chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 367,948 to 368,493
_313
Pf1N1B4_314 - Potassium-transporting ATPase B chain (EC 3.6.3.12) (TC 3.A.3.7.1), at 368,694 to 370,751
_314
Position (kb)
367
368
369 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.216 kb on + strand, within Pf1N1B4_312 at 367.217 kb on - strand, within Pf1N1B4_312 at 367.217 kb on - strand, within Pf1N1B4_312 at 367.282 kb on + strand, within Pf1N1B4_312 at 367.283 kb on - strand, within Pf1N1B4_312 at 367.283 kb on - strand, within Pf1N1B4_312 at 367.327 kb on - strand, within Pf1N1B4_312 at 367.690 kb on + strand at 367.691 kb on - strand at 367.691 kb on - strand at 367.725 kb on + strand at 367.725 kb on + strand at 367.725 kb on + strand at 367.813 kb on - strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.895 kb on + strand at 367.896 kb on - strand at 367.896 kb on - strand at 367.896 kb on - strand at 367.949 kb on + strand at 367.968 kb on + strand at 368.046 kb on + strand, within Pf1N1B4_313 at 368.047 kb on - strand, within Pf1N1B4_313 at 368.047 kb on - strand, within Pf1N1B4_313 at 368.177 kb on - strand, within Pf1N1B4_313 at 368.323 kb on + strand, within Pf1N1B4_313 at 368.426 kb on + strand, within Pf1N1B4_313 at 368.479 kb on + strand at 368.479 kb on + strand at 368.480 kb on - strand at 368.506 kb on + strand at 368.506 kb on + strand at 368.506 kb on + strand at 368.507 kb on - strand at 368.698 kb on - strand at 368.774 kb on - strand at 368.774 kb on - strand at 368.948 kb on - strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.983 kb on + strand, within Pf1N1B4_314 at 368.984 kb on - strand, within Pf1N1B4_314 at 368.984 kb on - strand, within Pf1N1B4_314 at 369.040 kb on - strand, within Pf1N1B4_314
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 367,216 + Pf1N1B4_312 0.78 +1.3 367,216 + Pf1N1B4_312 0.78 -0.1 367,216 + Pf1N1B4_312 0.78 -1.9 367,216 + Pf1N1B4_312 0.78 +1.9 367,216 + Pf1N1B4_312 0.78 -0.5 367,216 + Pf1N1B4_312 0.78 +0.6 367,217 - Pf1N1B4_312 0.78 -1.5 367,217 - Pf1N1B4_312 0.78 -1.3 367,282 + Pf1N1B4_312 0.81 -0.5 367,283 - Pf1N1B4_312 0.81 +0.4 367,283 - Pf1N1B4_312 0.81 -1.9 367,327 - Pf1N1B4_312 0.82 +0.1 367,690 + +2.1 367,691 - -0.3 367,691 - +0.8 367,725 + +0.6 367,725 + -0.1 367,725 + +1.9 367,813 - +0.1 367,895 + -0.3 367,895 + +1.9 367,895 + -1.2 367,895 + +1.1 367,895 + +0.4 367,895 + +0.5 367,895 + -0.7 367,895 + -0.5 367,895 + -1.2 367,895 + +0.8 367,895 + -1.9 367,895 + -0.3 367,895 + +1.4 367,895 + -0.9 367,895 + -1.8 367,895 + +1.7 367,896 - +0.1 367,896 - +1.1 367,896 - +0.5 367,949 + -0.9 367,968 + -1.2 368,046 + Pf1N1B4_313 0.18 -1.9 368,047 - Pf1N1B4_313 0.18 +1.1 368,047 - Pf1N1B4_313 0.18 -1.3 368,177 - Pf1N1B4_313 0.42 -2.5 368,323 + Pf1N1B4_313 0.69 +0.7 368,426 + Pf1N1B4_313 0.88 -0.8 368,479 + +0.5 368,479 + -0.9 368,480 - +0.3 368,506 + -0.3 368,506 + -2.8 368,506 + +0.1 368,507 - -3.0 368,698 - -1.7 368,774 - -0.9 368,774 - -2.2 368,948 - Pf1N1B4_314 0.12 -0.5 368,983 + Pf1N1B4_314 0.14 +1.3 368,983 + Pf1N1B4_314 0.14 -0.8 368,983 + Pf1N1B4_314 0.14 +1.7 368,983 + Pf1N1B4_314 0.14 +0.2 368,983 + Pf1N1B4_314 0.14 -3.0 368,984 - Pf1N1B4_314 0.14 -2.0 368,984 - Pf1N1B4_314 0.14 -2.7 369,040 - Pf1N1B4_314 0.17 -1.1
Or see this region's nucleotide sequence