Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_3033

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_3032 and Pf1N1B4_3033 are separated by 278 nucleotidesPf1N1B4_3033 and Pf1N1B4_3034 overlap by 1 nucleotidesPf1N1B4_3034 and Pf1N1B4_3035 overlap by 4 nucleotides Pf1N1B4_3032 - putative membrane protein, at 3,412,549 to 3,413,271 _3032 Pf1N1B4_3033 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 3,413,550 to 3,415,172 _3033 Pf1N1B4_3034 - Chemotaxis signal transduction protein, at 3,415,172 to 3,415,708 _3034 Pf1N1B4_3035 - Chemotaxis protein methyltransferase CheR (EC 2.1.1.80), at 3,415,705 to 3,416,994 _3035 Position (kb) 3413 3414 3415 3416Strain fitness (log2 ratio) -2 -1 0 1 2at 3412.577 kb on + strandat 3412.578 kb on - strandat 3412.578 kb on - strandat 3412.605 kb on + strandat 3412.606 kb on - strandat 3412.795 kb on + strand, within Pf1N1B4_3032at 3412.795 kb on + strand, within Pf1N1B4_3032at 3412.795 kb on + strandat 3412.796 kb on - strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.828 kb on + strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.829 kb on - strand, within Pf1N1B4_3032at 3412.837 kb on + strand, within Pf1N1B4_3032at 3413.134 kb on + strand, within Pf1N1B4_3032at 3413.134 kb on + strand, within Pf1N1B4_3032at 3413.135 kb on - strand, within Pf1N1B4_3032at 3413.135 kb on - strand, within Pf1N1B4_3032at 3413.135 kb on - strand, within Pf1N1B4_3032at 3413.135 kb on - strand, within Pf1N1B4_3032at 3413.185 kb on + strand, within Pf1N1B4_3032at 3413.185 kb on + strand, within Pf1N1B4_3032at 3413.269 kb on + strandat 3413.270 kb on - strandat 3413.343 kb on + strandat 3413.343 kb on + strandat 3413.343 kb on + strandat 3413.343 kb on + strandat 3413.343 kb on + strandat 3413.343 kb on + strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.344 kb on - strandat 3413.462 kb on - strandat 3413.513 kb on + strandat 3413.514 kb on - strandat 3413.514 kb on - strandat 3413.514 kb on - strandat 3413.700 kb on + strandat 3413.700 kb on + strandat 3413.700 kb on + strandat 3413.701 kb on - strandat 3413.701 kb on - strandat 3413.701 kb on - strandat 3413.701 kb on - strandat 3413.701 kb on - strandat 3413.701 kb on - strandat 3413.739 kb on + strand, within Pf1N1B4_3033at 3413.739 kb on + strand, within Pf1N1B4_3033at 3413.740 kb on - strand, within Pf1N1B4_3033at 3413.740 kb on - strand, within Pf1N1B4_3033at 3413.741 kb on + strand, within Pf1N1B4_3033at 3413.741 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.808 kb on + strand, within Pf1N1B4_3033at 3413.809 kb on - strand, within Pf1N1B4_3033at 3413.809 kb on - strand, within Pf1N1B4_3033at 3413.809 kb on - strand, within Pf1N1B4_3033at 3413.847 kb on + strand, within Pf1N1B4_3033at 3413.847 kb on + strand, within Pf1N1B4_3033at 3413.848 kb on - strand, within Pf1N1B4_3033at 3413.952 kb on + strand, within Pf1N1B4_3033at 3413.952 kb on + strand, within Pf1N1B4_3033at 3413.952 kb on + strand, within Pf1N1B4_3033at 3413.968 kb on - strand, within Pf1N1B4_3033at 3414.026 kb on + strand, within Pf1N1B4_3033at 3414.750 kb on + strand, within Pf1N1B4_3033at 3414.819 kb on + strand, within Pf1N1B4_3033at 3414.819 kb on + strand, within Pf1N1B4_3033at 3414.819 kb on + strand, within Pf1N1B4_3033at 3414.820 kb on - strand, within Pf1N1B4_3033at 3414.820 kb on - strandat 3414.820 kb on - strand, within Pf1N1B4_3033at 3414.820 kb on - strand, within Pf1N1B4_3033at 3414.820 kb on - strand, within Pf1N1B4_3033at 3415.606 kb on - strand, within Pf1N1B4_3034at 3415.606 kb on - strand, within Pf1N1B4_3034at 3415.659 kb on + strandat 3415.659 kb on + strandat 3415.660 kb on - strandat 3415.660 kb on - strandat 3415.660 kb on - strandat 3415.746 kb on + strandat 3415.747 kb on - strandat 3415.747 kb on - strandat 3415.840 kb on + strandat 3415.841 kb on - strand, within Pf1N1B4_3035at 3415.841 kb on - strand, within Pf1N1B4_3035at 3415.841 kb on - strand, within Pf1N1B4_3035at 3415.984 kb on - strand, within Pf1N1B4_3035at 3416.023 kb on - strand, within Pf1N1B4_3035at 3416.038 kb on + strand, within Pf1N1B4_3035at 3416.039 kb on - strand, within Pf1N1B4_3035at 3416.146 kb on + strand, within Pf1N1B4_3035at 3416.147 kb on - strand, within Pf1N1B4_3035at 3416.147 kb on - strand, within Pf1N1B4_3035at 3416.147 kb on - strand, within Pf1N1B4_3035

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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3,412,577 + +2.2
3,412,578 - +1.8
3,412,578 - -0.8
3,412,605 + +1.0
3,412,606 - -0.3
3,412,795 + Pf1N1B4_3032 0.34 -0.8
3,412,795 + Pf1N1B4_3032 0.34 -1.3
3,412,795 + -1.1
3,412,796 - Pf1N1B4_3032 0.34 -1.1
3,412,828 + Pf1N1B4_3032 0.39 -0.5
3,412,828 + Pf1N1B4_3032 0.39 +0.6
3,412,828 + Pf1N1B4_3032 0.39 -1.3
3,412,828 + Pf1N1B4_3032 0.39 +0.2
3,412,828 + Pf1N1B4_3032 0.39 +0.2
3,412,828 + Pf1N1B4_3032 0.39 -1.3
3,412,828 + Pf1N1B4_3032 0.39 +1.9
3,412,828 + Pf1N1B4_3032 0.39 -0.2
3,412,829 - Pf1N1B4_3032 0.39 -1.3
3,412,829 - Pf1N1B4_3032 0.39 -0.3
3,412,829 - Pf1N1B4_3032 0.39 -0.6
3,412,829 - Pf1N1B4_3032 0.39 -0.3
3,412,829 - Pf1N1B4_3032 0.39 -0.2
3,412,829 - Pf1N1B4_3032 0.39 -0.8
3,412,837 + Pf1N1B4_3032 0.40 -1.6
3,413,134 + Pf1N1B4_3032 0.81 -0.3
3,413,134 + Pf1N1B4_3032 0.81 -0.5
3,413,135 - Pf1N1B4_3032 0.81 +0.2
3,413,135 - Pf1N1B4_3032 0.81 -1.9
3,413,135 - Pf1N1B4_3032 0.81 +0.8
3,413,135 - Pf1N1B4_3032 0.81 -0.2
3,413,185 + Pf1N1B4_3032 0.88 +0.2
3,413,185 + Pf1N1B4_3032 0.88 -0.3
3,413,269 + +1.7
3,413,270 - -1.2
3,413,343 + -1.3
3,413,343 + -0.8
3,413,343 + -1.1
3,413,343 + -0.8
3,413,343 + -0.1
3,413,343 + -1.3
3,413,344 - +0.8
3,413,344 - -0.3
3,413,344 - -0.8
3,413,344 - -0.2
3,413,344 - -0.0
3,413,344 - +2.4
3,413,344 - -1.2
3,413,344 - -0.4
3,413,462 - -0.8
3,413,513 + -1.6
3,413,514 - -1.6
3,413,514 - +0.9
3,413,514 - -0.8
3,413,700 + -0.6
3,413,700 + -0.8
3,413,700 + -0.3
3,413,701 - -0.8
3,413,701 - -1.1
3,413,701 - -0.1
3,413,701 - +1.1
3,413,701 - +0.9
3,413,701 - +2.2
3,413,739 + Pf1N1B4_3033 0.12 -1.3
3,413,739 + Pf1N1B4_3033 0.12 +2.0
3,413,740 - Pf1N1B4_3033 0.12 -1.3
3,413,740 - Pf1N1B4_3033 0.12 +0.3
3,413,741 + Pf1N1B4_3033 0.12 -1.1
3,413,741 + Pf1N1B4_3033 0.12 -0.3
3,413,808 + Pf1N1B4_3033 0.16 +1.7
3,413,808 + Pf1N1B4_3033 0.16 +0.1
3,413,808 + Pf1N1B4_3033 0.16 +0.8
3,413,808 + Pf1N1B4_3033 0.16 -0.8
3,413,808 + Pf1N1B4_3033 0.16 -0.3
3,413,808 + Pf1N1B4_3033 0.16 +1.8
3,413,809 - Pf1N1B4_3033 0.16 -2.3
3,413,809 - Pf1N1B4_3033 0.16 +1.0
3,413,809 - Pf1N1B4_3033 0.16 +1.4
3,413,847 + Pf1N1B4_3033 0.18 -1.1
3,413,847 + Pf1N1B4_3033 0.18 +0.6
3,413,848 - Pf1N1B4_3033 0.18 -1.9
3,413,952 + Pf1N1B4_3033 0.25 -0.4
3,413,952 + Pf1N1B4_3033 0.25 -0.3
3,413,952 + Pf1N1B4_3033 0.25 +0.2
3,413,968 - Pf1N1B4_3033 0.26 +0.7
3,414,026 + Pf1N1B4_3033 0.29 +1.9
3,414,750 + Pf1N1B4_3033 0.74 -1.3
3,414,819 + Pf1N1B4_3033 0.78 +1.0
3,414,819 + Pf1N1B4_3033 0.78 -1.8
3,414,819 + Pf1N1B4_3033 0.78 +1.4
3,414,820 - Pf1N1B4_3033 0.78 +0.1
3,414,820 - -0.8
3,414,820 - Pf1N1B4_3033 0.78 -1.3
3,414,820 - Pf1N1B4_3033 0.78 -1.3
3,414,820 - Pf1N1B4_3033 0.78 -0.3
3,415,606 - Pf1N1B4_3034 0.81 -1.5
3,415,606 - Pf1N1B4_3034 0.81 -1.1
3,415,659 + -0.6
3,415,659 + +1.2
3,415,660 - +0.0
3,415,660 - +1.6
3,415,660 - +0.8
3,415,746 + -0.3
3,415,747 - +2.0
3,415,747 - +1.6
3,415,840 + +1.2
3,415,841 - Pf1N1B4_3035 0.11 -1.1
3,415,841 - Pf1N1B4_3035 0.11 -1.1
3,415,841 - Pf1N1B4_3035 0.11 -1.1
3,415,984 - Pf1N1B4_3035 0.22 +0.9
3,416,023 - Pf1N1B4_3035 0.25 -0.3
3,416,038 + Pf1N1B4_3035 0.26 -0.8
3,416,039 - Pf1N1B4_3035 0.26 +0.7
3,416,146 + Pf1N1B4_3035 0.34 +0.5
3,416,147 - Pf1N1B4_3035 0.34 -0.3
3,416,147 - Pf1N1B4_3035 0.34 -1.3
3,416,147 - Pf1N1B4_3035 0.34 -0.9

Or see this region's nucleotide sequence