Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2672 and Pf1N1B4_2673 are separated by 265 nucleotides Pf1N1B4_2673 and Pf1N1B4_2674 are separated by 103 nucleotides Pf1N1B4_2674 and Pf1N1B4_2675 overlap by 15 nucleotides
Pf1N1B4_2672 - Ethanolamine permease, at 3,008,802 to 3,010,172
_2672
Pf1N1B4_2673 - Aldehyde dehydrogenase (EC 1.2.1.3), at 3,010,438 to 3,011,958
_2673
Pf1N1B4_2674 - hypothetical protein, at 3,012,062 to 3,012,424
_2674
Pf1N1B4_2675 - Nitrogen regulation protein NR(I), at 3,012,410 to 3,014,323
_2675
Position (kb)
3010
3011
3012 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.484 kb on + strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.485 kb on - strand, within Pf1N1B4_2672 at 3009.577 kb on - strand, within Pf1N1B4_2672 at 3009.577 kb on - strand, within Pf1N1B4_2672 at 3009.607 kb on - strand, within Pf1N1B4_2672 at 3009.607 kb on - strand, within Pf1N1B4_2672 at 3009.759 kb on - strand, within Pf1N1B4_2672 at 3009.782 kb on - strand, within Pf1N1B4_2672 at 3009.835 kb on + strand, within Pf1N1B4_2672 at 3009.835 kb on + strand, within Pf1N1B4_2672 at 3009.836 kb on - strand, within Pf1N1B4_2672 at 3009.836 kb on - strand, within Pf1N1B4_2672 at 3009.941 kb on - strand, within Pf1N1B4_2672 at 3009.998 kb on + strand, within Pf1N1B4_2672 at 3009.998 kb on + strand, within Pf1N1B4_2672 at 3009.999 kb on - strand, within Pf1N1B4_2672 at 3009.999 kb on - strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.009 kb on + strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.010 kb on - strand, within Pf1N1B4_2672 at 3010.088 kb on - strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.125 kb on + strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.126 kb on - strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.160 kb on + strand at 3010.161 kb on - strand at 3010.161 kb on - strand at 3010.161 kb on - strand at 3010.163 kb on + strand at 3010.163 kb on + strand at 3010.163 kb on + strand at 3010.164 kb on - strand at 3010.164 kb on - strand at 3010.164 kb on - strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.172 kb on + strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.173 kb on - strand at 3010.193 kb on - strand at 3010.193 kb on - strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.280 kb on + strand at 3010.281 kb on - strand at 3010.281 kb on - strand at 3010.291 kb on + strand at 3010.336 kb on + strand at 3010.417 kb on + strand at 3010.417 kb on + strand at 3010.417 kb on + strand at 3010.418 kb on - strand at 3010.418 kb on - strand at 3010.466 kb on + strand at 3010.467 kb on - strand at 3010.467 kb on - strand at 3010.467 kb on - strand at 3010.548 kb on - strand at 3010.583 kb on + strand at 3010.728 kb on + strand, within Pf1N1B4_2673 at 3010.729 kb on - strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.808 kb on + strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.809 kb on - strand, within Pf1N1B4_2673 at 3010.811 kb on + strand, within Pf1N1B4_2673 at 3010.895 kb on + strand, within Pf1N1B4_2673 at 3011.019 kb on - strand, within Pf1N1B4_2673 at 3011.274 kb on + strand, within Pf1N1B4_2673 at 3011.412 kb on + strand, within Pf1N1B4_2673 at 3011.514 kb on - strand, within Pf1N1B4_2673 at 3011.820 kb on + strand at 3011.820 kb on + strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.821 kb on - strand at 3011.845 kb on - strand at 3011.845 kb on - strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.894 kb on + strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.895 kb on - strand at 3011.899 kb on - strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.903 kb on + strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.904 kb on - strand at 3011.951 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.986 kb on + strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.987 kb on - strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.988 kb on + strand at 3011.989 kb on - strand at 3012.016 kb on + strand at 3012.023 kb on - strand at 3012.058 kb on + strand at 3012.233 kb on + strand, within Pf1N1B4_2674 at 3012.234 kb on - strand, within Pf1N1B4_2674 at 3012.372 kb on - strand, within Pf1N1B4_2674 at 3012.401 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.404 kb on + strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.405 kb on - strand at 3012.536 kb on + strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.537 kb on - strand at 3012.806 kb on - strand, within Pf1N1B4_2675 at 3012.883 kb on + strand, within Pf1N1B4_2675 at 3012.884 kb on - strand, within Pf1N1B4_2675
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 3,009,484 + Pf1N1B4_2672 0.50 +2.6 3,009,484 + Pf1N1B4_2672 0.50 -0.5 3,009,484 + Pf1N1B4_2672 0.50 -0.0 3,009,485 - Pf1N1B4_2672 0.50 -0.3 3,009,485 - Pf1N1B4_2672 0.50 -1.7 3,009,485 - Pf1N1B4_2672 0.50 +1.6 3,009,485 - Pf1N1B4_2672 0.50 +0.5 3,009,577 - Pf1N1B4_2672 0.57 +0.8 3,009,577 - Pf1N1B4_2672 0.57 +0.9 3,009,607 - Pf1N1B4_2672 0.59 -0.6 3,009,607 - Pf1N1B4_2672 0.59 +0.0 3,009,759 - Pf1N1B4_2672 0.70 +1.4 3,009,782 - Pf1N1B4_2672 0.71 +0.6 3,009,835 + Pf1N1B4_2672 0.75 -0.5 3,009,835 + Pf1N1B4_2672 0.75 -0.3 3,009,836 - Pf1N1B4_2672 0.75 -3.3 3,009,836 - Pf1N1B4_2672 0.75 -2.0 3,009,941 - Pf1N1B4_2672 0.83 -0.6 3,009,998 + Pf1N1B4_2672 0.87 -1.7 3,009,998 + Pf1N1B4_2672 0.87 +1.4 3,009,999 - Pf1N1B4_2672 0.87 +2.3 3,009,999 - Pf1N1B4_2672 0.87 -0.3 3,010,009 + Pf1N1B4_2672 0.88 +1.2 3,010,009 + -0.1 3,010,009 + Pf1N1B4_2672 0.88 +0.6 3,010,009 + Pf1N1B4_2672 0.88 +0.7 3,010,009 + Pf1N1B4_2672 0.88 -1.4 3,010,009 + Pf1N1B4_2672 0.88 +0.5 3,010,009 + Pf1N1B4_2672 0.88 +0.8 3,010,009 + Pf1N1B4_2672 0.88 -0.1 3,010,009 + Pf1N1B4_2672 0.88 +0.9 3,010,010 - Pf1N1B4_2672 0.88 +0.8 3,010,010 - Pf1N1B4_2672 0.88 +0.9 3,010,010 - Pf1N1B4_2672 0.88 +0.4 3,010,088 - -2.3 3,010,125 + +0.9 3,010,125 + -1.0 3,010,125 + +0.1 3,010,125 + -0.4 3,010,125 + +2.4 3,010,125 + -1.6 3,010,125 + -0.2 3,010,125 + +2.0 3,010,126 - -0.1 3,010,126 - +1.9 3,010,126 - -0.9 3,010,126 - +0.7 3,010,126 - -0.1 3,010,126 - -0.9 3,010,160 + +0.0 3,010,160 + -1.7 3,010,160 + +0.4 3,010,160 + +0.3 3,010,160 + +0.9 3,010,161 - +2.4 3,010,161 - -0.1 3,010,161 - +1.7 3,010,163 + -1.7 3,010,163 + +0.9 3,010,163 + -1.7 3,010,164 - -1.6 3,010,164 - +1.2 3,010,164 - -1.6 3,010,172 + -1.1 3,010,172 + +0.1 3,010,172 + -0.2 3,010,172 + +2.2 3,010,172 + +1.6 3,010,173 - -0.6 3,010,173 - +0.4 3,010,173 - -0.9 3,010,173 - -0.9 3,010,193 - -1.5 3,010,193 - +1.6 3,010,280 + +3.2 3,010,280 + -0.6 3,010,280 + -1.2 3,010,280 + +1.0 3,010,280 + +1.0 3,010,280 + -0.4 3,010,281 - -0.1 3,010,281 - -1.1 3,010,291 + -1.1 3,010,336 + -0.6 3,010,417 + -1.1 3,010,417 + +0.4 3,010,417 + +0.4 3,010,418 - -0.2 3,010,418 - +0.3 3,010,466 + -0.6 3,010,467 - -1.1 3,010,467 - +0.7 3,010,467 - +0.1 3,010,548 - +1.4 3,010,583 + +1.1 3,010,728 + Pf1N1B4_2673 0.19 -1.7 3,010,729 - Pf1N1B4_2673 0.19 +2.4 3,010,808 + Pf1N1B4_2673 0.24 -0.6 3,010,808 + Pf1N1B4_2673 0.24 -0.0 3,010,808 + Pf1N1B4_2673 0.24 +1.7 3,010,808 + Pf1N1B4_2673 0.24 -0.1 3,010,808 + Pf1N1B4_2673 0.24 +1.4 3,010,808 + Pf1N1B4_2673 0.24 +0.9 3,010,808 + Pf1N1B4_2673 0.24 +1.4 3,010,808 + Pf1N1B4_2673 0.24 -0.1 3,010,808 + Pf1N1B4_2673 0.24 -1.6 3,010,808 + Pf1N1B4_2673 0.24 +0.8 3,010,808 + Pf1N1B4_2673 0.24 -0.1 3,010,808 + Pf1N1B4_2673 0.24 +1.4 3,010,808 + Pf1N1B4_2673 0.24 +1.7 3,010,808 + Pf1N1B4_2673 0.24 -1.9 3,010,809 - Pf1N1B4_2673 0.24 -1.9 3,010,809 - Pf1N1B4_2673 0.24 -0.9 3,010,809 - Pf1N1B4_2673 0.24 +0.4 3,010,809 - Pf1N1B4_2673 0.24 -1.1 3,010,809 - Pf1N1B4_2673 0.24 -1.9 3,010,809 - Pf1N1B4_2673 0.24 +3.4 3,010,809 - Pf1N1B4_2673 0.24 -2.0 3,010,809 - Pf1N1B4_2673 0.24 +2.4 3,010,809 - Pf1N1B4_2673 0.24 -0.5 3,010,809 - Pf1N1B4_2673 0.24 -0.3 3,010,809 - Pf1N1B4_2673 0.24 -0.9 3,010,811 + Pf1N1B4_2673 0.25 +0.2 3,010,895 + Pf1N1B4_2673 0.30 +0.4 3,011,019 - Pf1N1B4_2673 0.38 -1.4 3,011,274 + Pf1N1B4_2673 0.55 -0.1 3,011,412 + Pf1N1B4_2673 0.64 +0.9 3,011,514 - Pf1N1B4_2673 0.71 -0.4 3,011,820 + +0.4 3,011,820 + +1.4 3,011,821 - +0.8 3,011,821 - +0.2 3,011,821 - -1.7 3,011,821 - +0.6 3,011,821 - -0.2 3,011,821 - -0.7 3,011,845 - +0.4 3,011,845 - +0.4 3,011,894 + -0.9 3,011,894 + +0.7 3,011,894 + +0.6 3,011,894 + +0.4 3,011,894 + +0.6 3,011,894 + +1.1 3,011,894 + +2.2 3,011,894 + +0.4 3,011,894 + -0.3 3,011,894 + +0.3 3,011,894 + +2.0 3,011,895 - -1.7 3,011,895 - -1.2 3,011,895 - -0.6 3,011,895 - +1.4 3,011,895 - -1.4 3,011,895 - -0.4 3,011,895 - +1.4 3,011,895 - -0.6 3,011,899 - -2.4 3,011,903 + +0.4 3,011,903 + +1.1 3,011,903 + +1.1 3,011,903 + +1.0 3,011,903 + -0.9 3,011,903 + -2.9 3,011,903 + +0.2 3,011,904 - +1.4 3,011,904 - +0.2 3,011,904 - -1.7 3,011,951 + +0.8 3,011,986 + -1.2 3,011,986 + -2.3 3,011,986 + +0.7 3,011,986 + +1.1 3,011,986 + -0.2 3,011,986 + +1.4 3,011,986 + -1.6 3,011,987 - +0.1 3,011,987 - -0.9 3,011,987 - -0.2 3,011,987 - +2.3 3,011,988 + -1.1 3,011,988 + -0.1 3,011,988 + -2.4 3,011,988 + -1.0 3,011,989 - -0.9 3,012,016 + -0.3 3,012,023 - -0.2 3,012,058 + +1.4 3,012,233 + Pf1N1B4_2674 0.47 +0.2 3,012,234 - Pf1N1B4_2674 0.47 -1.4 3,012,372 - Pf1N1B4_2674 0.85 -0.6 3,012,401 + +0.4 3,012,404 + +0.7 3,012,404 + -1.7 3,012,404 + -1.4 3,012,405 - -0.6 3,012,405 - -1.4 3,012,405 - -0.9 3,012,536 + -0.4 3,012,537 - +1.2 3,012,537 - -1.2 3,012,537 - +0.7 3,012,806 - Pf1N1B4_2675 0.21 -1.4 3,012,883 + Pf1N1B4_2675 0.25 -0.6 3,012,884 - Pf1N1B4_2675 0.25 +0.8
Or see this region's nucleotide sequence