Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2624 and Pf1N1B4_2625 are separated by 54 nucleotides Pf1N1B4_2625 and Pf1N1B4_2626 overlap by 39 nucleotides Pf1N1B4_2626 and Pf1N1B4_2627 are separated by 12 nucleotides Pf1N1B4_2627 and Pf1N1B4_2628 overlap by 17 nucleotides Pf1N1B4_2628 and Pf1N1B4_2629 are separated by 17 nucleotides
Pf1N1B4_2624 - Transcriptional regulator containing an amidase domain and an AraC-type DNA-binding HTH domain, at 2,963,494 to 2,964,489
_2624
Pf1N1B4_2625 - hypothetical protein, at 2,964,544 to 2,964,834
_2625
Pf1N1B4_2626 - Biotin carboxylase (EC 6.3.4.14), at 2,964,796 to 2,965,881
_2626
Pf1N1B4_2627 - Prolyl oligopeptidase family protein, at 2,965,894 to 2,966,652
_2627
Pf1N1B4_2628 - hypothetical protein, at 2,966,636 to 2,966,764
_2628
Pf1N1B4_2629 - Inner membrane protein YbhQ, at 2,966,782 to 2,967,768
_2629
Position (kb)
2964
2965
2966 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.844 kb on + strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2963.845 kb on - strand, within Pf1N1B4_2624 at 2964.020 kb on + strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.021 kb on - strand, within Pf1N1B4_2624 at 2964.118 kb on - strand, within Pf1N1B4_2624 at 2964.494 kb on + strand at 2964.495 kb on - strand at 2964.495 kb on - strand at 2964.542 kb on + strand at 2964.643 kb on + strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.644 kb on - strand, within Pf1N1B4_2625 at 2964.665 kb on + strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.666 kb on - strand, within Pf1N1B4_2625 at 2964.690 kb on - strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.763 kb on + strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.764 kb on - strand, within Pf1N1B4_2625 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.931 kb on + strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.932 kb on - strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.964 kb on + strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2964.965 kb on - strand, within Pf1N1B4_2626 at 2965.072 kb on - strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.225 kb on + strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.226 kb on - strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.447 kb on + strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand at 2965.448 kb on - strand at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.448 kb on - strand, within Pf1N1B4_2626 at 2965.513 kb on + strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.514 kb on - strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.516 kb on + strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.517 kb on - strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand, within Pf1N1B4_2626 at 2965.618 kb on + strand at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.636 kb on + strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.637 kb on - strand, within Pf1N1B4_2626 at 2965.798 kb on + strand at 2965.798 kb on + strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.799 kb on - strand at 2965.854 kb on + strand at 2965.854 kb on + strand at 2965.854 kb on + strand at 2965.855 kb on - strand at 2965.855 kb on - strand at 2965.855 kb on - strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.875 kb on + strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.876 kb on - strand at 2965.879 kb on + strand at 2965.880 kb on - strand at 2965.893 kb on + strand at 2965.995 kb on - strand, within Pf1N1B4_2627 at 2966.224 kb on + strand, within Pf1N1B4_2627 at 2966.225 kb on - strand, within Pf1N1B4_2627 at 2966.234 kb on - strand, within Pf1N1B4_2627 at 2966.299 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.458 kb on + strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand, within Pf1N1B4_2627 at 2966.459 kb on - strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.578 kb on + strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.579 kb on - strand at 2966.637 kb on + strand at 2966.638 kb on - strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.837 kb on + strand at 2966.838 kb on - strand at 2966.838 kb on - strand at 2966.851 kb on - strand at 2966.873 kb on + strand at 2966.873 kb on + strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand at 2966.874 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,963,844 + Pf1N1B4_2624 0.35 +1.4 2,963,844 + Pf1N1B4_2624 0.35 -1.7 2,963,844 + Pf1N1B4_2624 0.35 +0.7 2,963,844 + Pf1N1B4_2624 0.35 -1.4 2,963,844 + Pf1N1B4_2624 0.35 +1.0 2,963,844 + Pf1N1B4_2624 0.35 +0.7 2,963,844 + Pf1N1B4_2624 0.35 -1.0 2,963,845 - Pf1N1B4_2624 0.35 -1.2 2,963,845 - Pf1N1B4_2624 0.35 +0.2 2,963,845 - Pf1N1B4_2624 0.35 -0.4 2,963,845 - Pf1N1B4_2624 0.35 -0.9 2,963,845 - Pf1N1B4_2624 0.35 -1.4 2,963,845 - Pf1N1B4_2624 0.35 -1.2 2,963,845 - Pf1N1B4_2624 0.35 +0.1 2,964,020 + Pf1N1B4_2624 0.53 +0.0 2,964,021 - Pf1N1B4_2624 0.53 +0.2 2,964,021 - Pf1N1B4_2624 0.53 -1.7 2,964,021 - Pf1N1B4_2624 0.53 +1.8 2,964,118 - Pf1N1B4_2624 0.63 +0.9 2,964,494 + +0.8 2,964,495 - +0.4 2,964,495 - -0.7 2,964,542 + -0.9 2,964,643 + Pf1N1B4_2625 0.34 -0.6 2,964,644 - Pf1N1B4_2625 0.34 -1.4 2,964,644 - Pf1N1B4_2625 0.34 -0.9 2,964,665 + Pf1N1B4_2625 0.42 -0.9 2,964,666 - Pf1N1B4_2625 0.42 -1.4 2,964,666 - Pf1N1B4_2625 0.42 -1.0 2,964,666 - Pf1N1B4_2625 0.42 -0.9 2,964,690 - Pf1N1B4_2625 0.50 -0.9 2,964,763 + Pf1N1B4_2625 0.75 +0.4 2,964,763 + Pf1N1B4_2625 0.75 -2.0 2,964,763 + Pf1N1B4_2625 0.75 -0.6 2,964,763 + Pf1N1B4_2625 0.75 -1.6 2,964,763 + Pf1N1B4_2625 0.75 +0.4 2,964,764 - Pf1N1B4_2625 0.76 +0.7 2,964,764 - Pf1N1B4_2625 0.76 +0.4 2,964,764 - Pf1N1B4_2625 0.76 +0.4 2,964,764 - Pf1N1B4_2625 0.76 -0.6 2,964,764 - Pf1N1B4_2625 0.76 -0.5 2,964,931 + Pf1N1B4_2626 0.12 +1.4 2,964,931 + Pf1N1B4_2626 0.12 +0.2 2,964,932 - Pf1N1B4_2626 0.13 +1.7 2,964,932 - Pf1N1B4_2626 0.13 -0.6 2,964,932 - Pf1N1B4_2626 0.13 -1.2 2,964,932 - Pf1N1B4_2626 0.13 -1.4 2,964,932 - Pf1N1B4_2626 0.13 -0.9 2,964,964 + Pf1N1B4_2626 0.15 +0.7 2,964,964 + Pf1N1B4_2626 0.15 +2.1 2,964,964 + Pf1N1B4_2626 0.15 +0.4 2,964,965 - Pf1N1B4_2626 0.16 -1.2 2,964,965 - Pf1N1B4_2626 0.16 +3.0 2,964,965 - Pf1N1B4_2626 0.16 +0.7 2,964,965 - Pf1N1B4_2626 0.16 -0.9 2,965,072 - Pf1N1B4_2626 0.25 -0.3 2,965,225 + Pf1N1B4_2626 0.40 +1.3 2,965,225 + Pf1N1B4_2626 0.40 +0.4 2,965,225 + Pf1N1B4_2626 0.40 -2.0 2,965,226 - Pf1N1B4_2626 0.40 -0.8 2,965,226 - Pf1N1B4_2626 0.40 +0.4 2,965,226 - Pf1N1B4_2626 0.40 +0.9 2,965,447 + Pf1N1B4_2626 0.60 +0.6 2,965,447 + Pf1N1B4_2626 0.60 +1.5 2,965,447 + Pf1N1B4_2626 0.60 -0.2 2,965,447 + Pf1N1B4_2626 0.60 +0.1 2,965,447 + Pf1N1B4_2626 0.60 -0.0 2,965,447 + Pf1N1B4_2626 0.60 +1.0 2,965,447 + Pf1N1B4_2626 0.60 -1.7 2,965,448 - Pf1N1B4_2626 0.60 +1.4 2,965,448 - Pf1N1B4_2626 0.60 -0.4 2,965,448 - -0.2 2,965,448 - +2.4 2,965,448 - Pf1N1B4_2626 0.60 -2.5 2,965,448 - Pf1N1B4_2626 0.60 +0.6 2,965,513 + Pf1N1B4_2626 0.66 +1.1 2,965,514 - Pf1N1B4_2626 0.66 +2.0 2,965,514 - Pf1N1B4_2626 0.66 -2.2 2,965,516 + Pf1N1B4_2626 0.66 +1.8 2,965,516 + Pf1N1B4_2626 0.66 -0.9 2,965,516 + Pf1N1B4_2626 0.66 -1.0 2,965,516 + Pf1N1B4_2626 0.66 +0.2 2,965,517 - Pf1N1B4_2626 0.66 -0.3 2,965,517 - Pf1N1B4_2626 0.66 +1.0 2,965,517 - Pf1N1B4_2626 0.66 +0.2 2,965,618 + Pf1N1B4_2626 0.76 -0.6 2,965,618 + Pf1N1B4_2626 0.76 -0.5 2,965,618 + +0.4 2,965,636 + Pf1N1B4_2626 0.77 -2.7 2,965,636 + Pf1N1B4_2626 0.77 -1.9 2,965,636 + Pf1N1B4_2626 0.77 +0.7 2,965,636 + Pf1N1B4_2626 0.77 -0.3 2,965,636 + Pf1N1B4_2626 0.77 -0.2 2,965,637 - Pf1N1B4_2626 0.77 +0.6 2,965,637 - Pf1N1B4_2626 0.77 -0.2 2,965,637 - Pf1N1B4_2626 0.77 -1.4 2,965,637 - Pf1N1B4_2626 0.77 +2.0 2,965,637 - Pf1N1B4_2626 0.77 -1.4 2,965,798 + +0.2 2,965,798 + +0.2 2,965,799 - +0.9 2,965,799 - -2.2 2,965,799 - -2.7 2,965,799 - +1.0 2,965,854 + +0.1 2,965,854 + +1.2 2,965,854 + +1.8 2,965,855 - +1.3 2,965,855 - +1.9 2,965,855 - +0.6 2,965,875 + +0.1 2,965,875 + +1.4 2,965,875 + +0.1 2,965,875 + +1.3 2,965,875 + +1.6 2,965,876 - +1.2 2,965,876 - +0.0 2,965,876 - +0.3 2,965,876 - -0.3 2,965,879 + -0.3 2,965,880 - +0.4 2,965,893 + -2.0 2,965,995 - Pf1N1B4_2627 0.13 -0.9 2,966,224 + Pf1N1B4_2627 0.43 +1.4 2,966,225 - Pf1N1B4_2627 0.44 -1.6 2,966,234 - Pf1N1B4_2627 0.45 -0.9 2,966,299 + Pf1N1B4_2627 0.53 -0.1 2,966,458 + Pf1N1B4_2627 0.74 -2.4 2,966,458 + Pf1N1B4_2627 0.74 +1.8 2,966,458 + Pf1N1B4_2627 0.74 +0.7 2,966,458 + Pf1N1B4_2627 0.74 -0.6 2,966,458 + Pf1N1B4_2627 0.74 -0.9 2,966,458 + Pf1N1B4_2627 0.74 +0.3 2,966,458 + Pf1N1B4_2627 0.74 +0.4 2,966,459 - Pf1N1B4_2627 0.74 +1.4 2,966,459 - Pf1N1B4_2627 0.74 -1.2 2,966,459 - Pf1N1B4_2627 0.74 -0.7 2,966,459 - Pf1N1B4_2627 0.74 +0.4 2,966,459 - Pf1N1B4_2627 0.74 -1.4 2,966,459 - Pf1N1B4_2627 0.74 +1.2 2,966,459 - Pf1N1B4_2627 0.74 +0.7 2,966,459 - Pf1N1B4_2627 0.74 -3.4 2,966,459 - +1.8 2,966,578 + +0.9 2,966,578 + -1.2 2,966,578 + +1.0 2,966,578 + +0.1 2,966,578 + -0.9 2,966,578 + -0.7 2,966,578 + +2.9 2,966,578 + -0.3 2,966,579 - -1.2 2,966,579 - +1.3 2,966,579 - -0.4 2,966,579 - +5.2 2,966,579 - +0.4 2,966,637 + +0.7 2,966,638 - +0.1 2,966,837 + -0.3 2,966,837 + +0.7 2,966,837 + +2.7 2,966,837 + +0.4 2,966,837 + +0.4 2,966,837 + +1.0 2,966,837 + +1.1 2,966,838 - -1.9 2,966,838 - -1.1 2,966,851 - +0.1 2,966,873 + -1.4 2,966,873 + +2.4 2,966,874 - -1.2 2,966,874 - -1.4 2,966,874 - +0.7 2,966,874 - +1.1
Or see this region's nucleotide sequence