Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2557 and Pf1N1B4_2558 are separated by 33 nucleotides Pf1N1B4_2558 and Pf1N1B4_2559 are separated by 135 nucleotides Pf1N1B4_2559 and Pf1N1B4_2560 are separated by 13 nucleotides Pf1N1B4_2560 and Pf1N1B4_2561 are separated by 38 nucleotides Pf1N1B4_2561 and Pf1N1B4_2562 are separated by 74 nucleotides
Pf1N1B4_2557 - 2-nitropropane dioxygenase (EC 1.13.11.32), at 2,899,322 to 2,900,290
_2557
Pf1N1B4_2558 - Protoporphyrinogen IX oxidase, novel form, HemJ (EC 1.3.-.-), at 2,900,324 to 2,900,755
_2558
Pf1N1B4_2559 - N-acetyl-gamma-glutamyl-phosphate reductase (EC 1.2.1.38), at 2,900,891 to 2,901,925
_2559
Pf1N1B4_2560 - hypothetical protein, at 2,901,939 to 2,902,055
_2560
Pf1N1B4_2561 - probable iron binding protein from the HesB_IscA_SufA family, at 2,902,094 to 2,902,444
_2561
Pf1N1B4_2562 - Anhydro-N-acetylmuramic acid kinase (EC 2.7.1.-), at 2,902,519 to 2,903,610
_2562
Position (kb)
2900
2901
2902 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2899.941 kb on + strand, within Pf1N1B4_2557 at 2900.000 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand, within Pf1N1B4_2557 at 2900.076 kb on + strand at 2900.180 kb on + strand, within Pf1N1B4_2557 at 2900.224 kb on + strand at 2900.225 kb on - strand at 2900.243 kb on + strand at 2900.244 kb on - strand at 2900.244 kb on - strand at 2900.325 kb on + strand at 2900.325 kb on + strand at 2900.326 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.760 kb on - strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.789 kb on + strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.790 kb on - strand at 2900.792 kb on + strand at 2900.792 kb on + strand at 2900.849 kb on + strand at 2900.849 kb on + strand at 2900.890 kb on + strand at 2900.890 kb on + strand at 2902.001 kb on + strand, within Pf1N1B4_2560 at 2902.002 kb on - strand at 2902.002 kb on - strand, within Pf1N1B4_2560 at 2902.003 kb on + strand, within Pf1N1B4_2560 at 2902.003 kb on + strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.004 kb on - strand, within Pf1N1B4_2560 at 2902.008 kb on + strand, within Pf1N1B4_2560 at 2902.009 kb on - strand, within Pf1N1B4_2560 at 2902.014 kb on + strand, within Pf1N1B4_2560 at 2902.063 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.459 kb on + strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.460 kb on - strand at 2902.462 kb on + strand at 2902.463 kb on - strand at 2902.463 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,899,941 + Pf1N1B4_2557 0.64 -0.2 2,899,941 + Pf1N1B4_2557 0.64 +1.4 2,899,941 + Pf1N1B4_2557 0.64 +1.3 2,900,000 + Pf1N1B4_2557 0.70 -2.0 2,900,076 + Pf1N1B4_2557 0.78 +0.3 2,900,076 + +0.8 2,900,180 + Pf1N1B4_2557 0.89 +0.5 2,900,224 + +0.8 2,900,225 - +1.4 2,900,243 + -1.3 2,900,244 - +1.9 2,900,244 - -1.9 2,900,325 + +0.2 2,900,325 + +0.6 2,900,326 - -0.2 2,900,760 - -1.2 2,900,760 - +1.4 2,900,760 - -0.9 2,900,789 + +0.8 2,900,789 + -2.0 2,900,789 + -0.2 2,900,789 + -2.2 2,900,789 + -0.6 2,900,789 + +1.2 2,900,789 + -2.3 2,900,789 + +1.1 2,900,789 + +1.4 2,900,789 + -1.6 2,900,789 + -0.8 2,900,789 + +0.4 2,900,789 + -1.7 2,900,789 + -1.4 2,900,789 + -2.0 2,900,789 + -1.9 2,900,790 - -0.9 2,900,790 - +2.0 2,900,790 - +0.1 2,900,790 - +1.1 2,900,790 - +1.4 2,900,792 + -0.6 2,900,792 + -0.6 2,900,849 + +0.2 2,900,849 + -0.3 2,900,890 + +0.8 2,900,890 + +0.8 2,902,001 + Pf1N1B4_2560 0.53 -1.4 2,902,002 - +1.4 2,902,002 - Pf1N1B4_2560 0.54 +0.4 2,902,003 + Pf1N1B4_2560 0.55 -2.2 2,902,003 + Pf1N1B4_2560 0.55 -1.2 2,902,004 - Pf1N1B4_2560 0.56 -2.0 2,902,004 - Pf1N1B4_2560 0.56 +0.3 2,902,004 - Pf1N1B4_2560 0.56 +0.4 2,902,004 - Pf1N1B4_2560 0.56 +1.6 2,902,004 - Pf1N1B4_2560 0.56 -0.3 2,902,004 - Pf1N1B4_2560 0.56 -0.3 2,902,004 - Pf1N1B4_2560 0.56 -0.3 2,902,004 - Pf1N1B4_2560 0.56 +0.6 2,902,004 - Pf1N1B4_2560 0.56 +0.6 2,902,004 - Pf1N1B4_2560 0.56 +2.3 2,902,004 - Pf1N1B4_2560 0.56 -2.7 2,902,004 - Pf1N1B4_2560 0.56 -1.2 2,902,008 + Pf1N1B4_2560 0.59 -1.9 2,902,009 - Pf1N1B4_2560 0.60 +0.4 2,902,014 + Pf1N1B4_2560 0.64 -1.7 2,902,063 + -0.2 2,902,459 + -0.3 2,902,459 + +2.0 2,902,459 + -1.5 2,902,459 + -1.2 2,902,460 - -0.9 2,902,460 - +0.6 2,902,460 - -0.7 2,902,460 - +0.9 2,902,460 - -1.6 2,902,460 - -0.6 2,902,462 + +0.3 2,902,463 - +0.9 2,902,463 - +1.6
Or see this region's nucleotide sequence