Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2473 and Pf1N1B4_2474 overlap by 4 nucleotides Pf1N1B4_2474 and Pf1N1B4_2475 are separated by 291 nucleotides Pf1N1B4_2475 and Pf1N1B4_2476 overlap by 4 nucleotides
Pf1N1B4_2473 - Transmembrane sensor, at 2,805,111 to 2,806,085
_2473
Pf1N1B4_2474 - RNA polymerase sigma-70 factor, ECF subfamily, at 2,806,082 to 2,806,558
_2474
Pf1N1B4_2475 - DNA polymerase III epsilon subunit (EC 2.7.7.7), at 2,806,850 to 2,807,557
_2475
Pf1N1B4_2476 - Predicted signal-transduction protein containing cAMP-binding and CBS domains, at 2,807,554 to 2,809,494
_2476
Position (kb)
2806
2807
2808 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2806.098 kb on + strand at 2806.098 kb on + strand at 2806.098 kb on + strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.099 kb on - strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.110 kb on + strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.111 kb on - strand at 2806.137 kb on + strand, within Pf1N1B4_2474 at 2806.138 kb on - strand, within Pf1N1B4_2474 at 2806.212 kb on + strand, within Pf1N1B4_2474 at 2806.212 kb on + strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.213 kb on - strand, within Pf1N1B4_2474 at 2806.347 kb on + strand, within Pf1N1B4_2474 at 2806.347 kb on + strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.348 kb on - strand, within Pf1N1B4_2474 at 2806.377 kb on + strand, within Pf1N1B4_2474 at 2806.378 kb on - strand, within Pf1N1B4_2474 at 2806.378 kb on - strand, within Pf1N1B4_2474 at 2806.532 kb on + strand at 2806.532 kb on + strand at 2806.533 kb on - strand at 2806.533 kb on - strand at 2806.554 kb on + strand at 2806.669 kb on + strand at 2806.669 kb on + strand at 2806.670 kb on - strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.851 kb on + strand at 2806.915 kb on + strand at 2806.916 kb on - strand at 2807.121 kb on + strand, within Pf1N1B4_2475 at 2807.122 kb on - strand, within Pf1N1B4_2475 at 2807.395 kb on + strand, within Pf1N1B4_2475 at 2807.429 kb on + strand, within Pf1N1B4_2475 at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.557 kb on + strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.558 kb on - strand at 2807.692 kb on + strand at 2807.693 kb on - strand at 2807.693 kb on - strand at 2807.732 kb on + strand at 2808.064 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.080 kb on + strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476 at 2808.081 kb on - strand, within Pf1N1B4_2476
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,806,098 + +0.7 2,806,098 + +0.1 2,806,098 + -0.4 2,806,099 - -0.9 2,806,099 - +0.1 2,806,099 - +0.1 2,806,099 - +1.7 2,806,099 - -1.9 2,806,110 + +1.0 2,806,110 + +0.5 2,806,110 + +0.5 2,806,110 + -0.1 2,806,110 + -1.7 2,806,110 + -1.1 2,806,110 + -3.2 2,806,111 - +0.5 2,806,111 - -0.1 2,806,111 - +0.7 2,806,111 - +0.0 2,806,111 - +0.7 2,806,137 + Pf1N1B4_2474 0.12 -0.9 2,806,138 - Pf1N1B4_2474 0.12 -0.1 2,806,212 + Pf1N1B4_2474 0.27 -2.2 2,806,212 + Pf1N1B4_2474 0.27 +0.5 2,806,213 - Pf1N1B4_2474 0.27 -1.4 2,806,213 - Pf1N1B4_2474 0.27 +2.7 2,806,213 - Pf1N1B4_2474 0.27 -1.2 2,806,213 - Pf1N1B4_2474 0.27 +2.3 2,806,347 + Pf1N1B4_2474 0.56 -0.9 2,806,347 + Pf1N1B4_2474 0.56 -0.5 2,806,348 - Pf1N1B4_2474 0.56 +1.6 2,806,348 - Pf1N1B4_2474 0.56 +0.2 2,806,348 - Pf1N1B4_2474 0.56 -0.9 2,806,348 - Pf1N1B4_2474 0.56 -0.5 2,806,377 + Pf1N1B4_2474 0.62 +0.2 2,806,378 - Pf1N1B4_2474 0.62 -0.5 2,806,378 - Pf1N1B4_2474 0.62 +1.0 2,806,532 + +1.0 2,806,532 + -2.1 2,806,533 - -0.8 2,806,533 - -0.3 2,806,554 + -0.0 2,806,669 + +0.3 2,806,669 + -0.2 2,806,670 - +0.2 2,806,851 + +1.0 2,806,851 + +0.7 2,806,851 + -0.5 2,806,915 + -0.5 2,806,916 - -0.5 2,807,121 + Pf1N1B4_2475 0.38 +0.7 2,807,122 - Pf1N1B4_2475 0.38 -1.4 2,807,395 + Pf1N1B4_2475 0.77 -1.4 2,807,429 + Pf1N1B4_2475 0.82 +0.5 2,807,557 + -1.9 2,807,557 + +3.0 2,807,557 + +2.2 2,807,558 - +1.6 2,807,558 - -1.5 2,807,558 - +1.9 2,807,692 + -0.5 2,807,693 - -1.4 2,807,693 - +0.6 2,807,732 + -0.7 2,808,064 + Pf1N1B4_2476 0.26 -2.9 2,808,080 + Pf1N1B4_2476 0.27 -0.5 2,808,080 + Pf1N1B4_2476 0.27 +0.5 2,808,080 + Pf1N1B4_2476 0.27 -1.2 2,808,080 + Pf1N1B4_2476 0.27 -0.1 2,808,081 - Pf1N1B4_2476 0.27 +2.1 2,808,081 - Pf1N1B4_2476 0.27 +0.1 2,808,081 - Pf1N1B4_2476 0.27 +0.7 2,808,081 - +3.0 2,808,081 - Pf1N1B4_2476 0.27 -0.9 2,808,081 - Pf1N1B4_2476 0.27 -1.5 2,808,081 - Pf1N1B4_2476 0.27 -0.1
Or see this region's nucleotide sequence