Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2411 and Pf1N1B4_2412 are separated by 131 nucleotides Pf1N1B4_2412 and Pf1N1B4_2413 are separated by 183 nucleotides
Pf1N1B4_2411 - FIG00953568: hypothetical protein, at 2,731,030 to 2,732,208
_2411
Pf1N1B4_2412 - ATP-dependent 23S rRNA helicase DbpA, at 2,732,340 to 2,733,725
_2412
Pf1N1B4_2413 - Permeases of the major facilitator superfamily, at 2,733,909 to 2,735,336
_2413
Position (kb)
2732
2733
2734 Strain fitness (log2 ratio)
-2
-1
0
1 at 2731.980 kb on - strand, within Pf1N1B4_2411 at 2732.379 kb on - strand at 2732.434 kb on + strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.435 kb on - strand at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.527 kb on - strand, within Pf1N1B4_2412 at 2732.792 kb on + strand, within Pf1N1B4_2412 at 2732.843 kb on + strand at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.844 kb on - strand, within Pf1N1B4_2412 at 2732.869 kb on - strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.055 kb on + strand, within Pf1N1B4_2412 at 2733.056 kb on - strand, within Pf1N1B4_2412 at 2733.227 kb on - strand, within Pf1N1B4_2412 at 2733.698 kb on + strand at 2733.698 kb on + strand at 2733.701 kb on + strand at 2733.701 kb on + strand at 2733.702 kb on - strand at 2733.702 kb on - strand at 2733.702 kb on - strand at 2733.760 kb on + strand at 2733.760 kb on + strand at 2733.760 kb on + strand at 2733.761 kb on - strand at 2733.761 kb on - strand at 2733.790 kb on - strand at 2733.800 kb on - strand at 2733.807 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,731,980 - Pf1N1B4_2411 0.81 -1.1 2,732,379 - -1.9 2,732,434 + -1.6 2,732,435 - -0.9 2,732,435 - +1.4 2,732,435 - -1.1 2,732,527 - Pf1N1B4_2412 0.13 -0.3 2,732,527 - Pf1N1B4_2412 0.13 -0.6 2,732,527 - Pf1N1B4_2412 0.13 -1.6 2,732,527 - Pf1N1B4_2412 0.13 -1.4 2,732,792 + Pf1N1B4_2412 0.33 +0.4 2,732,843 + -0.6 2,732,844 - Pf1N1B4_2412 0.36 -0.6 2,732,844 - Pf1N1B4_2412 0.36 -2.5 2,732,869 - Pf1N1B4_2412 0.38 -2.1 2,733,055 + Pf1N1B4_2412 0.52 -1.4 2,733,055 + Pf1N1B4_2412 0.52 -0.1 2,733,055 + Pf1N1B4_2412 0.52 -1.1 2,733,056 - Pf1N1B4_2412 0.52 -0.7 2,733,227 - Pf1N1B4_2412 0.64 +0.4 2,733,698 + -0.1 2,733,698 + -0.0 2,733,701 + +1.4 2,733,701 + +1.0 2,733,702 - -0.3 2,733,702 - +0.2 2,733,702 - +1.1 2,733,760 + -2.1 2,733,760 + +0.7 2,733,760 + -0.8 2,733,761 - -2.0 2,733,761 - +1.0 2,733,790 - -0.9 2,733,800 - -0.2 2,733,807 - +1.6
Or see this region's nucleotide sequence