Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2298 and Pf1N1B4_2299 overlap by 8 nucleotides Pf1N1B4_2299 and Pf1N1B4_2300 are separated by 85 nucleotides Pf1N1B4_2300 and Pf1N1B4_2301 are separated by 110 nucleotides
Pf1N1B4_2298 - probable integral membrane protein NMA1898, at 2,615,717 to 2,617,093
_2298
Pf1N1B4_2299 - probable integral membrane protein NMA1899, at 2,617,086 to 2,618,465
_2299
Pf1N1B4_2300 - Dihydrofolate reductase (EC 1.5.1.3), at 2,618,551 to 2,619,063
_2300
Pf1N1B4_2301 - L-cystine uptake protein TcyP, at 2,619,174 to 2,620,565
_2301
Position (kb)
2617
2618
2619 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.255 kb on + strand, within Pf1N1B4_2298 at 2616.256 kb on - strand, within Pf1N1B4_2298 at 2616.386 kb on + strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.387 kb on - strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.504 kb on + strand, within Pf1N1B4_2298 at 2616.720 kb on + strand, within Pf1N1B4_2298 at 2616.720 kb on + strand at 2616.720 kb on + strand, within Pf1N1B4_2298 at 2616.721 kb on - strand, within Pf1N1B4_2298 at 2616.865 kb on - strand, within Pf1N1B4_2298 at 2616.865 kb on - strand, within Pf1N1B4_2298 at 2617.045 kb on + strand at 2617.153 kb on - strand at 2617.377 kb on + strand, within Pf1N1B4_2299 at 2617.378 kb on - strand, within Pf1N1B4_2299 at 2617.449 kb on + strand, within Pf1N1B4_2299 at 2617.450 kb on - strand, within Pf1N1B4_2299 at 2618.056 kb on - strand, within Pf1N1B4_2299 at 2618.056 kb on - strand, within Pf1N1B4_2299 at 2618.119 kb on - strand, within Pf1N1B4_2299 at 2618.119 kb on - strand, within Pf1N1B4_2299 at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.418 kb on + strand at 2618.419 kb on - strand at 2618.419 kb on - strand at 2618.482 kb on + strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.483 kb on - strand at 2618.500 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand at 2619.062 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,616,255 + Pf1N1B4_2298 0.39 -1.5 2,616,255 + Pf1N1B4_2298 0.39 +0.5 2,616,255 + Pf1N1B4_2298 0.39 +0.9 2,616,255 + Pf1N1B4_2298 0.39 -1.3 2,616,255 + Pf1N1B4_2298 0.39 -2.2 2,616,256 - Pf1N1B4_2298 0.39 -1.3 2,616,386 + Pf1N1B4_2298 0.49 +1.8 2,616,387 - Pf1N1B4_2298 0.49 -1.1 2,616,387 - Pf1N1B4_2298 0.49 +2.4 2,616,387 - Pf1N1B4_2298 0.49 -0.9 2,616,504 + Pf1N1B4_2298 0.57 -0.9 2,616,504 + Pf1N1B4_2298 0.57 +1.1 2,616,504 + Pf1N1B4_2298 0.57 +2.0 2,616,720 + Pf1N1B4_2298 0.73 +1.5 2,616,720 + +0.9 2,616,720 + Pf1N1B4_2298 0.73 -0.4 2,616,721 - Pf1N1B4_2298 0.73 -1.7 2,616,865 - Pf1N1B4_2298 0.83 -2.0 2,616,865 - Pf1N1B4_2298 0.83 -1.3 2,617,045 + -1.5 2,617,153 - +0.2 2,617,377 + Pf1N1B4_2299 0.21 +0.3 2,617,378 - Pf1N1B4_2299 0.21 -1.5 2,617,449 + Pf1N1B4_2299 0.26 -2.4 2,617,450 - Pf1N1B4_2299 0.26 -0.9 2,618,056 - Pf1N1B4_2299 0.70 -0.5 2,618,056 - Pf1N1B4_2299 0.70 -0.5 2,618,119 - Pf1N1B4_2299 0.75 -1.3 2,618,119 - Pf1N1B4_2299 0.75 -4.2 2,618,418 + -0.5 2,618,418 + +1.5 2,618,418 + -0.3 2,618,418 + -0.1 2,618,419 - -1.9 2,618,419 - -0.9 2,618,482 + -1.2 2,618,483 - -0.5 2,618,483 - +1.5 2,618,483 - -1.9 2,618,483 - +0.1 2,618,500 - -1.3 2,619,062 - +0.9 2,619,062 - -1.7 2,619,062 - -0.5 2,619,062 - +0.9 2,619,062 - +0.1 2,619,062 - +1.4
Or see this region's nucleotide sequence