Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2104 and Pf1N1B4_2105 are separated by 78 nucleotides Pf1N1B4_2105 and Pf1N1B4_2106 are separated by 9 nucleotides
Pf1N1B4_2104 - Predicted signal transduction protein, at 2,415,662 to 2,417,062
_2104
Pf1N1B4_2105 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 2,417,141 to 2,419,216
_2105
Pf1N1B4_2106 - Hydrogen peroxide-inducible genes activator, at 2,419,226 to 2,420,155
_2106
Position (kb)
2417
2418
2419
2420 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2416.179 kb on + strand, within Pf1N1B4_2104 at 2416.179 kb on + strand, within Pf1N1B4_2104 at 2416.179 kb on + strand, within Pf1N1B4_2104 at 2416.180 kb on - strand, within Pf1N1B4_2104 at 2416.341 kb on + strand, within Pf1N1B4_2104 at 2416.342 kb on - strand, within Pf1N1B4_2104 at 2416.342 kb on - strand, within Pf1N1B4_2104 at 2416.401 kb on - strand, within Pf1N1B4_2104 at 2416.731 kb on + strand, within Pf1N1B4_2104 at 2416.732 kb on - strand, within Pf1N1B4_2104 at 2416.802 kb on + strand, within Pf1N1B4_2104 at 2416.821 kb on - strand, within Pf1N1B4_2104 at 2416.939 kb on + strand at 2416.980 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.095 kb on + strand at 2417.096 kb on - strand at 2417.096 kb on - strand at 2417.096 kb on - strand at 2417.096 kb on - strand at 2417.096 kb on - strand at 2417.701 kb on - strand, within Pf1N1B4_2105 at 2417.701 kb on - strand, within Pf1N1B4_2105 at 2418.435 kb on + strand, within Pf1N1B4_2105 at 2418.435 kb on + strand, within Pf1N1B4_2105 at 2418.826 kb on - strand, within Pf1N1B4_2105 at 2418.838 kb on - strand, within Pf1N1B4_2105 at 2419.660 kb on - strand, within Pf1N1B4_2106 at 2419.660 kb on - strand, within Pf1N1B4_2106 at 2419.660 kb on - strand, within Pf1N1B4_2106 at 2419.660 kb on - strand, within Pf1N1B4_2106 at 2419.788 kb on + strand, within Pf1N1B4_2106 at 2419.789 kb on - strand, within Pf1N1B4_2106 at 2419.789 kb on - strand, within Pf1N1B4_2106 at 2419.789 kb on - strand, within Pf1N1B4_2106 at 2419.789 kb on - strand, within Pf1N1B4_2106 at 2420.134 kb on - strand at 2420.134 kb on - strand at 2420.138 kb on + strand at 2420.138 kb on + strand at 2420.155 kb on + strand at 2420.155 kb on + strand at 2420.156 kb on - strand at 2420.156 kb on - strand at 2420.156 kb on - strand at 2420.188 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,416,179 + Pf1N1B4_2104 0.37 +0.7 2,416,179 + Pf1N1B4_2104 0.37 +0.6 2,416,179 + Pf1N1B4_2104 0.37 +0.7 2,416,180 - Pf1N1B4_2104 0.37 +2.1 2,416,341 + Pf1N1B4_2104 0.48 +3.6 2,416,342 - Pf1N1B4_2104 0.49 -1.0 2,416,342 - Pf1N1B4_2104 0.49 +0.8 2,416,401 - Pf1N1B4_2104 0.53 -0.7 2,416,731 + Pf1N1B4_2104 0.76 -1.0 2,416,732 - Pf1N1B4_2104 0.76 -2.3 2,416,802 + Pf1N1B4_2104 0.81 -1.7 2,416,821 - Pf1N1B4_2104 0.83 +1.4 2,416,939 + -0.4 2,416,980 + -0.7 2,417,095 + +1.2 2,417,095 + -0.0 2,417,095 + +0.2 2,417,095 + -2.5 2,417,095 + +0.3 2,417,095 + -0.0 2,417,095 + +0.4 2,417,095 + +1.6 2,417,095 + -1.7 2,417,096 - -0.4 2,417,096 - +0.6 2,417,096 - -1.9 2,417,096 - -0.2 2,417,096 - +2.6 2,417,701 - Pf1N1B4_2105 0.27 -1.0 2,417,701 - Pf1N1B4_2105 0.27 -1.6 2,418,435 + Pf1N1B4_2105 0.62 +0.6 2,418,435 + Pf1N1B4_2105 0.62 -0.5 2,418,826 - Pf1N1B4_2105 0.81 +0.6 2,418,838 - Pf1N1B4_2105 0.82 -1.7 2,419,660 - Pf1N1B4_2106 0.47 -2.0 2,419,660 - Pf1N1B4_2106 0.47 -1.6 2,419,660 - Pf1N1B4_2106 0.47 -1.6 2,419,660 - Pf1N1B4_2106 0.47 -2.2 2,419,788 + Pf1N1B4_2106 0.60 -2.0 2,419,789 - Pf1N1B4_2106 0.61 -0.0 2,419,789 - Pf1N1B4_2106 0.61 -2.2 2,419,789 - Pf1N1B4_2106 0.61 -1.6 2,419,789 - Pf1N1B4_2106 0.61 -1.2 2,420,134 - -1.0 2,420,134 - -0.7 2,420,138 + -1.2 2,420,138 + -1.7 2,420,155 + -1.0 2,420,155 + -0.0 2,420,156 - -1.0 2,420,156 - -1.2 2,420,156 - +1.0 2,420,188 - +0.3
Or see this region's nucleotide sequence