Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_2104

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_2103 and Pf1N1B4_2104 are separated by 209 nucleotidesPf1N1B4_2104 and Pf1N1B4_2105 are separated by 78 nucleotides Pf1N1B4_2103 - Superfamily II DNA and RNA helicases, at 2,415,075 to 2,415,452 _2103 Pf1N1B4_2104 - Predicted signal transduction protein, at 2,415,662 to 2,417,062 _2104 Pf1N1B4_2105 - ATP-dependent DNA helicase RecG (EC 3.6.1.-), at 2,417,141 to 2,419,216 _2105 Position (kb) 2415 2416 2417 2418Strain fitness (log2 ratio) -2 -1 0 1 2 3at 2414.750 kb on + strandat 2414.750 kb on + strandat 2414.750 kb on + strandat 2414.751 kb on - strandat 2414.751 kb on - strandat 2414.751 kb on - strandat 2414.839 kb on - strandat 2414.839 kb on - strandat 2414.862 kb on + strandat 2414.862 kb on + strandat 2414.995 kb on + strandat 2415.034 kb on - strandat 2415.134 kb on + strand, within Pf1N1B4_2103at 2415.134 kb on + strand, within Pf1N1B4_2103at 2415.135 kb on - strand, within Pf1N1B4_2103at 2415.184 kb on + strand, within Pf1N1B4_2103at 2415.184 kb on + strand, within Pf1N1B4_2103at 2415.184 kb on + strand, within Pf1N1B4_2103at 2415.184 kb on + strand, within Pf1N1B4_2103at 2415.185 kb on - strand, within Pf1N1B4_2103at 2415.239 kb on + strand, within Pf1N1B4_2103at 2415.240 kb on - strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.258 kb on + strand, within Pf1N1B4_2103at 2415.259 kb on - strand, within Pf1N1B4_2103at 2415.259 kb on - strand, within Pf1N1B4_2103at 2415.344 kb on + strand, within Pf1N1B4_2103at 2415.344 kb on + strand, within Pf1N1B4_2103at 2415.345 kb on - strand, within Pf1N1B4_2103at 2415.345 kb on - strand, within Pf1N1B4_2103at 2415.345 kb on - strand, within Pf1N1B4_2103at 2415.423 kb on + strandat 2415.423 kb on + strandat 2415.423 kb on + strandat 2415.424 kb on - strandat 2415.434 kb on + strandat 2415.434 kb on + strandat 2415.784 kb on + strandat 2415.785 kb on - strandat 2415.785 kb on - strandat 2415.785 kb on - strandat 2415.818 kb on - strandat 2415.876 kb on + strand, within Pf1N1B4_2104at 2415.877 kb on - strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.993 kb on + strand, within Pf1N1B4_2104at 2415.994 kb on - strand, within Pf1N1B4_2104at 2415.994 kb on - strand, within Pf1N1B4_2104at 2415.994 kb on - strand, within Pf1N1B4_2104at 2415.994 kb on - strand, within Pf1N1B4_2104at 2415.994 kb on - strand, within Pf1N1B4_2104at 2416.077 kb on + strand, within Pf1N1B4_2104at 2416.107 kb on + strand, within Pf1N1B4_2104at 2416.107 kb on + strand, within Pf1N1B4_2104at 2416.107 kb on + strand, within Pf1N1B4_2104at 2416.108 kb on - strand, within Pf1N1B4_2104at 2416.179 kb on + strand, within Pf1N1B4_2104at 2416.179 kb on + strand, within Pf1N1B4_2104at 2416.179 kb on + strand, within Pf1N1B4_2104at 2416.180 kb on - strand, within Pf1N1B4_2104at 2416.341 kb on + strand, within Pf1N1B4_2104at 2416.342 kb on - strand, within Pf1N1B4_2104at 2416.342 kb on - strand, within Pf1N1B4_2104at 2416.401 kb on - strand, within Pf1N1B4_2104at 2416.731 kb on + strand, within Pf1N1B4_2104at 2416.732 kb on - strand, within Pf1N1B4_2104at 2416.802 kb on + strand, within Pf1N1B4_2104at 2416.821 kb on - strand, within Pf1N1B4_2104at 2416.939 kb on + strandat 2416.980 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.095 kb on + strandat 2417.096 kb on - strandat 2417.096 kb on - strandat 2417.096 kb on - strandat 2417.096 kb on - strandat 2417.096 kb on - strandat 2417.701 kb on - strand, within Pf1N1B4_2105at 2417.701 kb on - strand, within Pf1N1B4_2105

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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2,414,750 + -0.0
2,414,750 + -1.4
2,414,750 + -1.5
2,414,751 - -0.7
2,414,751 - +1.5
2,414,751 - +0.2
2,414,839 - -1.4
2,414,839 - -1.0
2,414,862 + +0.3
2,414,862 + -0.4
2,414,995 + +3.6
2,415,034 - +1.6
2,415,134 + Pf1N1B4_2103 0.16 +0.4
2,415,134 + Pf1N1B4_2103 0.16 -0.0
2,415,135 - Pf1N1B4_2103 0.16 +0.3
2,415,184 + Pf1N1B4_2103 0.29 -1.0
2,415,184 + Pf1N1B4_2103 0.29 -1.6
2,415,184 + Pf1N1B4_2103 0.29 -1.1
2,415,184 + Pf1N1B4_2103 0.29 -1.0
2,415,185 - Pf1N1B4_2103 0.29 +0.6
2,415,239 + Pf1N1B4_2103 0.43 +0.6
2,415,240 - Pf1N1B4_2103 0.44 -0.4
2,415,258 + Pf1N1B4_2103 0.48 -0.3
2,415,258 + Pf1N1B4_2103 0.48 -0.0
2,415,258 + Pf1N1B4_2103 0.48 -0.8
2,415,258 + Pf1N1B4_2103 0.48 +1.4
2,415,258 + Pf1N1B4_2103 0.48 +0.1
2,415,258 + Pf1N1B4_2103 0.48 +0.3
2,415,258 + Pf1N1B4_2103 0.48 -0.0
2,415,258 + Pf1N1B4_2103 0.48 +1.8
2,415,259 - Pf1N1B4_2103 0.49 +0.8
2,415,259 - Pf1N1B4_2103 0.49 +1.4
2,415,344 + Pf1N1B4_2103 0.71 +1.0
2,415,344 + Pf1N1B4_2103 0.71 -0.4
2,415,345 - Pf1N1B4_2103 0.71 +0.7
2,415,345 - Pf1N1B4_2103 0.71 +0.6
2,415,345 - Pf1N1B4_2103 0.71 +0.8
2,415,423 + -0.4
2,415,423 + -0.2
2,415,423 + -0.0
2,415,424 - -2.1
2,415,434 + -0.8
2,415,434 + -0.2
2,415,784 + +2.6
2,415,785 - -1.6
2,415,785 - +2.6
2,415,785 - +0.2
2,415,818 - -1.6
2,415,876 + Pf1N1B4_2104 0.15 -0.6
2,415,877 - Pf1N1B4_2104 0.15 +0.2
2,415,993 + Pf1N1B4_2104 0.24 -0.7
2,415,993 + Pf1N1B4_2104 0.24 +0.3
2,415,993 + Pf1N1B4_2104 0.24 +1.2
2,415,993 + Pf1N1B4_2104 0.24 +1.2
2,415,993 + Pf1N1B4_2104 0.24 -0.0
2,415,993 + Pf1N1B4_2104 0.24 -1.1
2,415,993 + Pf1N1B4_2104 0.24 -2.2
2,415,994 - Pf1N1B4_2104 0.24 +1.3
2,415,994 - Pf1N1B4_2104 0.24 -1.1
2,415,994 - Pf1N1B4_2104 0.24 -0.4
2,415,994 - Pf1N1B4_2104 0.24 -2.0
2,415,994 - Pf1N1B4_2104 0.24 +2.2
2,416,077 + Pf1N1B4_2104 0.30 -1.5
2,416,107 + Pf1N1B4_2104 0.32 -1.6
2,416,107 + Pf1N1B4_2104 0.32 -0.5
2,416,107 + Pf1N1B4_2104 0.32 -1.1
2,416,108 - Pf1N1B4_2104 0.32 +0.6
2,416,179 + Pf1N1B4_2104 0.37 +0.7
2,416,179 + Pf1N1B4_2104 0.37 +0.6
2,416,179 + Pf1N1B4_2104 0.37 +0.7
2,416,180 - Pf1N1B4_2104 0.37 +2.1
2,416,341 + Pf1N1B4_2104 0.48 +3.6
2,416,342 - Pf1N1B4_2104 0.49 -1.0
2,416,342 - Pf1N1B4_2104 0.49 +0.8
2,416,401 - Pf1N1B4_2104 0.53 -0.7
2,416,731 + Pf1N1B4_2104 0.76 -1.0
2,416,732 - Pf1N1B4_2104 0.76 -2.3
2,416,802 + Pf1N1B4_2104 0.81 -1.7
2,416,821 - Pf1N1B4_2104 0.83 +1.4
2,416,939 + -0.4
2,416,980 + -0.7
2,417,095 + +1.2
2,417,095 + -0.0
2,417,095 + +0.2
2,417,095 + -2.5
2,417,095 + +0.3
2,417,095 + -0.0
2,417,095 + +0.4
2,417,095 + +1.6
2,417,095 + -1.7
2,417,096 - -0.4
2,417,096 - +0.6
2,417,096 - -1.9
2,417,096 - -0.2
2,417,096 - +2.6
2,417,701 - Pf1N1B4_2105 0.27 -1.0
2,417,701 - Pf1N1B4_2105 0.27 -1.6

Or see this region's nucleotide sequence