Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2035 and Pf1N1B4_2036 are separated by 15 nucleotides Pf1N1B4_2036 and Pf1N1B4_2037 are separated by 178 nucleotides
Pf1N1B4_2035 - Phosphate transport system permease protein PstC (TC 3.A.1.7.1), at 2,322,044 to 2,324,332
_2035
Pf1N1B4_2036 - Phosphate transport system permease protein PstA (TC 3.A.1.7.1), at 2,324,348 to 2,326,018
_2036
Pf1N1B4_2037 - Phosphate transport ATP-binding protein PstB (TC 3.A.1.7.1), at 2,326,197 to 2,327,030
_2037
Position (kb)
2324
2325
2326
2327 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2323.358 kb on + strand, within Pf1N1B4_2035 at 2323.358 kb on + strand, within Pf1N1B4_2035 at 2323.448 kb on + strand, within Pf1N1B4_2035 at 2323.502 kb on + strand, within Pf1N1B4_2035 at 2323.503 kb on - strand, within Pf1N1B4_2035 at 2323.610 kb on + strand, within Pf1N1B4_2035 at 2323.787 kb on + strand, within Pf1N1B4_2035 at 2323.787 kb on + strand, within Pf1N1B4_2035 at 2323.799 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.865 kb on + strand, within Pf1N1B4_2035 at 2323.866 kb on - strand, within Pf1N1B4_2035 at 2323.928 kb on + strand, within Pf1N1B4_2035 at 2323.929 kb on - strand, within Pf1N1B4_2035 at 2323.929 kb on - strand, within Pf1N1B4_2035 at 2324.318 kb on + strand at 2324.330 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2324.339 kb on + strand at 2325.194 kb on + strand, within Pf1N1B4_2036 at 2325.195 kb on - strand at 2325.341 kb on + strand, within Pf1N1B4_2036 at 2325.341 kb on + strand, within Pf1N1B4_2036 at 2325.341 kb on + strand, within Pf1N1B4_2036 at 2325.342 kb on - strand, within Pf1N1B4_2036 at 2325.342 kb on - strand, within Pf1N1B4_2036 at 2325.353 kb on + strand, within Pf1N1B4_2036 at 2325.353 kb on + strand, within Pf1N1B4_2036 at 2325.353 kb on + strand, within Pf1N1B4_2036 at 2325.428 kb on + strand, within Pf1N1B4_2036 at 2325.458 kb on + strand at 2325.818 kb on + strand, within Pf1N1B4_2036 at 2325.818 kb on + strand, within Pf1N1B4_2036 at 2325.863 kb on + strand at 2325.863 kb on + strand at 2325.863 kb on + strand at 2325.914 kb on + strand at 2325.971 kb on + strand at 2325.998 kb on + strand at 2325.998 kb on + strand at 2326.012 kb on + strand at 2326.139 kb on + strand at 2326.139 kb on + strand at 2326.139 kb on + strand at 2326.494 kb on + strand, within Pf1N1B4_2037 at 2326.495 kb on - strand, within Pf1N1B4_2037 at 2326.593 kb on + strand, within Pf1N1B4_2037 at 2326.594 kb on - strand, within Pf1N1B4_2037 at 2326.777 kb on + strand, within Pf1N1B4_2037 at 2326.778 kb on - strand, within Pf1N1B4_2037 at 2326.932 kb on + strand, within Pf1N1B4_2037 at 2326.932 kb on + strand, within Pf1N1B4_2037 at 2326.932 kb on + strand, within Pf1N1B4_2037 at 2326.932 kb on + strand, within Pf1N1B4_2037 at 2326.933 kb on - strand, within Pf1N1B4_2037
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,323,358 + Pf1N1B4_2035 0.57 -1.7 2,323,358 + Pf1N1B4_2035 0.57 +0.6 2,323,448 + Pf1N1B4_2035 0.61 -0.1 2,323,502 + Pf1N1B4_2035 0.64 -1.0 2,323,503 - Pf1N1B4_2035 0.64 -1.2 2,323,610 + Pf1N1B4_2035 0.68 -1.2 2,323,787 + Pf1N1B4_2035 0.76 -0.1 2,323,787 + Pf1N1B4_2035 0.76 -1.7 2,323,799 + Pf1N1B4_2035 0.77 -1.6 2,323,865 + Pf1N1B4_2035 0.80 +0.0 2,323,865 + Pf1N1B4_2035 0.80 -0.6 2,323,865 + Pf1N1B4_2035 0.80 +0.3 2,323,865 + Pf1N1B4_2035 0.80 +1.3 2,323,866 - Pf1N1B4_2035 0.80 -0.7 2,323,928 + Pf1N1B4_2035 0.82 +0.2 2,323,929 - Pf1N1B4_2035 0.82 -3.3 2,323,929 - Pf1N1B4_2035 0.82 +0.6 2,324,318 + +0.8 2,324,330 + +0.7 2,324,339 + +0.1 2,324,339 + -0.4 2,324,339 + +0.4 2,324,339 + +1.6 2,324,339 + -0.6 2,324,339 + -3.3 2,325,194 + Pf1N1B4_2036 0.51 +0.4 2,325,195 - -0.7 2,325,341 + Pf1N1B4_2036 0.59 +2.2 2,325,341 + Pf1N1B4_2036 0.59 +0.1 2,325,341 + Pf1N1B4_2036 0.59 -0.1 2,325,342 - Pf1N1B4_2036 0.59 +0.0 2,325,342 - Pf1N1B4_2036 0.59 -3.0 2,325,353 + Pf1N1B4_2036 0.60 +0.0 2,325,353 + Pf1N1B4_2036 0.60 +0.6 2,325,353 + Pf1N1B4_2036 0.60 +1.4 2,325,428 + Pf1N1B4_2036 0.65 -1.7 2,325,458 + +0.0 2,325,818 + Pf1N1B4_2036 0.88 -0.3 2,325,818 + Pf1N1B4_2036 0.88 +0.1 2,325,863 + +1.2 2,325,863 + +1.9 2,325,863 + -0.1 2,325,914 + -0.6 2,325,971 + +0.9 2,325,998 + -1.7 2,325,998 + +0.6 2,326,012 + +1.0 2,326,139 + -0.2 2,326,139 + +0.2 2,326,139 + +0.6 2,326,494 + Pf1N1B4_2037 0.36 -2.9 2,326,495 - Pf1N1B4_2037 0.36 -2.1 2,326,593 + Pf1N1B4_2037 0.47 +3.0 2,326,594 - Pf1N1B4_2037 0.48 -0.4 2,326,777 + Pf1N1B4_2037 0.70 -2.7 2,326,778 - Pf1N1B4_2037 0.70 -1.4 2,326,932 + Pf1N1B4_2037 0.88 -0.7 2,326,932 + Pf1N1B4_2037 0.88 +0.3 2,326,932 + Pf1N1B4_2037 0.88 -0.4 2,326,932 + Pf1N1B4_2037 0.88 -1.2 2,326,933 - Pf1N1B4_2037 0.88 +1.3
Or see this region's nucleotide sequence