Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_2026 and Pf1N1B4_2027 are separated by 334 nucleotides Pf1N1B4_2027 and Pf1N1B4_2028 are separated by 11 nucleotides
Pf1N1B4_2026 - Transcriptional regulator, at 2,313,420 to 2,314,337
_2026
Pf1N1B4_2027 - Phosphoribosylaminoimidazole carboxylase catalytic subunit (EC 4.1.1.21), at 2,314,672 to 2,315,163
_2027
Pf1N1B4_2028 - Phosphoribosylaminoimidazole carboxylase ATPase subunit (EC 4.1.1.21), at 2,315,175 to 2,316,257
_2028
Position (kb)
2314
2315
2316 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2313.867 kb on + strand, within Pf1N1B4_2026 at 2313.867 kb on + strand at 2313.867 kb on + strand, within Pf1N1B4_2026 at 2314.018 kb on - strand, within Pf1N1B4_2026 at 2314.082 kb on + strand, within Pf1N1B4_2026 at 2314.083 kb on - strand, within Pf1N1B4_2026 at 2314.088 kb on + strand, within Pf1N1B4_2026 at 2314.088 kb on + strand, within Pf1N1B4_2026 at 2314.088 kb on + strand, within Pf1N1B4_2026 at 2314.088 kb on + strand, within Pf1N1B4_2026 at 2314.089 kb on - strand, within Pf1N1B4_2026 at 2314.089 kb on - strand, within Pf1N1B4_2026 at 2314.089 kb on - strand at 2314.092 kb on + strand, within Pf1N1B4_2026 at 2314.092 kb on + strand, within Pf1N1B4_2026 at 2314.331 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.455 kb on + strand at 2314.456 kb on - strand at 2314.456 kb on - strand at 2314.484 kb on + strand at 2314.484 kb on + strand at 2314.506 kb on - strand at 2314.534 kb on + strand at 2314.535 kb on - strand at 2314.535 kb on - strand at 2314.551 kb on + strand at 2314.551 kb on + strand at 2314.551 kb on + strand at 2314.551 kb on + strand at 2314.552 kb on - strand at 2314.552 kb on - strand at 2314.553 kb on + strand at 2314.553 kb on + strand at 2314.553 kb on + strand at 2314.553 kb on + strand at 2314.553 kb on + strand at 2314.587 kb on + strand at 2314.594 kb on + strand at 2315.763 kb on + strand, within Pf1N1B4_2028
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,313,867 + Pf1N1B4_2026 0.49 -1.6 2,313,867 + +0.0 2,313,867 + Pf1N1B4_2026 0.49 -1.9 2,314,018 - Pf1N1B4_2026 0.65 +0.0 2,314,082 + Pf1N1B4_2026 0.72 +0.4 2,314,083 - Pf1N1B4_2026 0.72 -1.9 2,314,088 + Pf1N1B4_2026 0.73 -0.4 2,314,088 + Pf1N1B4_2026 0.73 -1.7 2,314,088 + Pf1N1B4_2026 0.73 +0.0 2,314,088 + Pf1N1B4_2026 0.73 -1.4 2,314,089 - Pf1N1B4_2026 0.73 -0.5 2,314,089 - Pf1N1B4_2026 0.73 -0.4 2,314,089 - +0.6 2,314,092 + Pf1N1B4_2026 0.73 +0.0 2,314,092 + Pf1N1B4_2026 0.73 -1.0 2,314,331 + +1.9 2,314,455 + +1.9 2,314,455 + -1.0 2,314,455 + -1.6 2,314,455 + +0.0 2,314,455 + -0.7 2,314,455 + +0.3 2,314,455 + +0.9 2,314,455 + +2.8 2,314,456 - +0.7 2,314,456 - -0.7 2,314,484 + +1.1 2,314,484 + +2.0 2,314,506 - +1.0 2,314,534 + -1.0 2,314,535 - -1.6 2,314,535 - -0.1 2,314,551 + -1.2 2,314,551 + -0.1 2,314,551 + -1.4 2,314,551 + +2.2 2,314,552 - +0.4 2,314,552 - +1.0 2,314,553 + +0.3 2,314,553 + +0.0 2,314,553 + -1.7 2,314,553 + +0.0 2,314,553 + +0.0 2,314,587 + -1.6 2,314,594 + +1.5 2,315,763 + Pf1N1B4_2028 0.54 +0.0
Or see this region's nucleotide sequence