Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1873 and Pf1N1B4_1874 are separated by 59 nucleotides Pf1N1B4_1874 and Pf1N1B4_1875 are separated by 74 nucleotides Pf1N1B4_1875 and Pf1N1B4_1876 are separated by 300 nucleotides
Pf1N1B4_1873 - Homoserine kinase (EC 2.7.1.39), at 2,142,647 to 2,143,597
_1873
Pf1N1B4_1874 - FIG00953549: hypothetical protein, at 2,143,657 to 2,143,944
_1874
Pf1N1B4_1875 - DNA polymerase I (EC 2.7.7.7), at 2,144,019 to 2,146,787
_1875
Pf1N1B4_1876 - GTP-binding protein EngB, at 2,147,088 to 2,147,729
_1876
Position (kb)
2144
2145
2146
2147 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 2143.643 kb on - strand at 2143.643 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.648 kb on - strand at 2143.659 kb on - strand at 2143.670 kb on - strand at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand, within Pf1N1B4_1874 at 2143.752 kb on - strand at 2143.788 kb on - strand, within Pf1N1B4_1874 at 2143.846 kb on + strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.847 kb on - strand, within Pf1N1B4_1874 at 2143.933 kb on + strand at 2143.934 kb on - strand at 2143.947 kb on + strand at 2146.818 kb on + strand at 2146.846 kb on + strand at 2146.846 kb on + strand at 2146.847 kb on - strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.848 kb on + strand at 2146.849 kb on - strand at 2146.870 kb on + strand at 2146.871 kb on - strand at 2146.892 kb on + strand at 2146.902 kb on + strand at 2146.962 kb on - strand at 2146.972 kb on - strand at 2147.081 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 2,143,643 - -0.3 2,143,643 - -0.1 2,143,648 - -0.2 2,143,648 - +0.9 2,143,648 - +0.7 2,143,648 - +2.4 2,143,648 - -2.4 2,143,659 - +0.6 2,143,670 - +0.6 2,143,752 - Pf1N1B4_1874 0.33 -0.7 2,143,752 - Pf1N1B4_1874 0.33 +1.4 2,143,752 - Pf1N1B4_1874 0.33 -0.4 2,143,752 - +1.6 2,143,788 - Pf1N1B4_1874 0.45 -1.1 2,143,846 + Pf1N1B4_1874 0.66 -1.4 2,143,847 - Pf1N1B4_1874 0.66 -1.2 2,143,847 - Pf1N1B4_1874 0.66 +2.2 2,143,847 - Pf1N1B4_1874 0.66 +1.3 2,143,847 - Pf1N1B4_1874 0.66 +1.4 2,143,847 - +2.6 2,143,847 - Pf1N1B4_1874 0.66 -0.7 2,143,847 - Pf1N1B4_1874 0.66 -0.6 2,143,847 - Pf1N1B4_1874 0.66 -2.5 2,143,847 - Pf1N1B4_1874 0.66 +1.6 2,143,933 + -0.4 2,143,934 - +1.8 2,143,947 + -0.4 2,146,818 + -0.4 2,146,846 + +0.8 2,146,846 + +3.9 2,146,847 - -0.0 2,146,848 + -0.3 2,146,848 + -0.7 2,146,848 + -0.2 2,146,848 + -1.4 2,146,848 + +0.4 2,146,848 + +0.4 2,146,848 + -2.9 2,146,849 - +2.1 2,146,870 + -1.5 2,146,871 - +0.6 2,146,892 + -0.6 2,146,902 + -2.0 2,146,962 - -0.0 2,146,972 - -0.4 2,147,081 + -2.3
Or see this region's nucleotide sequence