Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1745 and Pf1N1B4_1746 are separated by 418 nucleotides Pf1N1B4_1746 and Pf1N1B4_1747 are separated by 181 nucleotides Pf1N1B4_1747 and Pf1N1B4_1748 are separated by 5 nucleotides
Pf1N1B4_1745 - Biofilm PGA outer membrane secretin PgaA, at 1,995,429 to 1,997,909
_1745
Pf1N1B4_1746 - GTP-binding protein, at 1,998,328 to 1,999,803
_1746
Pf1N1B4_1747 - hypothetical protein, at 1,999,985 to 2,000,116
_1747
Pf1N1B4_1748 - NADH:ubiquinone oxidoreductase subunit 2 (chain N), at 2,000,122 to 2,000,757
_1748
Position (kb)
1998
1999
2000 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1997.339 kb on - strand, within Pf1N1B4_1745 at 1997.356 kb on + strand, within Pf1N1B4_1745 at 1997.356 kb on + strand, within Pf1N1B4_1745 at 1997.357 kb on - strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.542 kb on + strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.543 kb on - strand, within Pf1N1B4_1745 at 1997.544 kb on + strand, within Pf1N1B4_1745 at 1997.544 kb on + strand, within Pf1N1B4_1745 at 1997.545 kb on - strand, within Pf1N1B4_1745 at 1997.645 kb on - strand, within Pf1N1B4_1745 at 1997.645 kb on - strand, within Pf1N1B4_1745 at 1997.745 kb on + strand at 1997.745 kb on + strand at 1997.745 kb on + strand at 1997.746 kb on - strand at 1997.746 kb on - strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.764 kb on + strand at 1997.765 kb on - strand at 1997.800 kb on + strand at 1997.871 kb on + strand at 1997.885 kb on + strand at 1997.885 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.900 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.923 kb on + strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1997.924 kb on - strand at 1998.000 kb on - strand at 1998.033 kb on + strand at 1998.034 kb on - strand at 1998.034 kb on - strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.047 kb on + strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.048 kb on - strand at 1998.141 kb on + strand at 1998.141 kb on + strand at 1998.142 kb on - strand at 1998.142 kb on - strand at 1998.290 kb on - strand at 1998.323 kb on - strand at 1998.435 kb on - strand at 1998.435 kb on - strand at 1999.007 kb on + strand, within Pf1N1B4_1746 at 1999.145 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.154 kb on + strand, within Pf1N1B4_1746 at 1999.155 kb on - strand, within Pf1N1B4_1746 at 1999.254 kb on + strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.255 kb on - strand, within Pf1N1B4_1746 at 1999.256 kb on + strand, within Pf1N1B4_1746 at 1999.360 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.481 kb on + strand, within Pf1N1B4_1746 at 1999.482 kb on - strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.639 kb on + strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.640 kb on - strand, within Pf1N1B4_1746 at 1999.767 kb on - strand at 1999.813 kb on + strand at 1999.814 kb on - strand at 1999.816 kb on + strand at 1999.816 kb on + strand at 1999.817 kb on - strand at 1999.833 kb on + strand at 1999.833 kb on + strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.834 kb on - strand at 1999.844 kb on + strand at 1999.986 kb on + strand at 2000.129 kb on + strand at 2000.129 kb on + strand at 2000.130 kb on - strand at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.187 kb on + strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.188 kb on - strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.189 kb on + strand, within Pf1N1B4_1748 at 2000.190 kb on - strand, within Pf1N1B4_1748 at 2000.457 kb on - strand, within Pf1N1B4_1748 at 2000.472 kb on - strand, within Pf1N1B4_1748 at 2000.578 kb on + strand, within Pf1N1B4_1748 at 2000.579 kb on - strand, within Pf1N1B4_1748 at 2000.645 kb on - strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.690 kb on + strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.691 kb on - strand, within Pf1N1B4_1748 at 2000.758 kb on + strand at 2000.758 kb on + strand at 2000.759 kb on - strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.761 kb on + strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand at 2000.762 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,997,339 - Pf1N1B4_1745 0.77 -0.8 1,997,356 + Pf1N1B4_1745 0.78 -0.5 1,997,356 + Pf1N1B4_1745 0.78 -1.0 1,997,357 - Pf1N1B4_1745 0.78 -1.2 1,997,542 + Pf1N1B4_1745 0.85 +0.3 1,997,542 + Pf1N1B4_1745 0.85 +3.1 1,997,542 + Pf1N1B4_1745 0.85 -1.9 1,997,542 + Pf1N1B4_1745 0.85 +1.3 1,997,542 + Pf1N1B4_1745 0.85 +2.2 1,997,542 + Pf1N1B4_1745 0.85 -0.4 1,997,542 + Pf1N1B4_1745 0.85 +0.8 1,997,542 + Pf1N1B4_1745 0.85 -1.5 1,997,542 + Pf1N1B4_1745 0.85 +1.3 1,997,542 + Pf1N1B4_1745 0.85 +0.8 1,997,542 + Pf1N1B4_1745 0.85 -0.5 1,997,542 + Pf1N1B4_1745 0.85 -0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.9 1,997,542 + Pf1N1B4_1745 0.85 +0.6 1,997,542 + Pf1N1B4_1745 0.85 +0.7 1,997,542 + Pf1N1B4_1745 0.85 -0.0 1,997,542 + Pf1N1B4_1745 0.85 +0.7 1,997,542 + Pf1N1B4_1745 0.85 -1.8 1,997,543 - Pf1N1B4_1745 0.85 -1.2 1,997,543 - Pf1N1B4_1745 0.85 -1.3 1,997,543 - Pf1N1B4_1745 0.85 -1.9 1,997,543 - Pf1N1B4_1745 0.85 +1.0 1,997,543 - Pf1N1B4_1745 0.85 -0.5 1,997,543 - Pf1N1B4_1745 0.85 -1.0 1,997,544 + Pf1N1B4_1745 0.85 -0.5 1,997,544 + Pf1N1B4_1745 0.85 -0.9 1,997,545 - Pf1N1B4_1745 0.85 -0.0 1,997,645 - Pf1N1B4_1745 0.89 -0.6 1,997,645 - Pf1N1B4_1745 0.89 +1.1 1,997,745 + +0.1 1,997,745 + -1.0 1,997,745 + +0.5 1,997,746 - +1.7 1,997,746 - +0.5 1,997,764 + +0.8 1,997,764 + +0.5 1,997,764 + +1.1 1,997,764 + +0.2 1,997,764 + +1.1 1,997,764 + -0.5 1,997,765 - +0.7 1,997,800 + +0.0 1,997,871 + -1.0 1,997,885 + -0.5 1,997,885 + -0.5 1,997,900 + +0.2 1,997,900 + -0.0 1,997,900 + +0.3 1,997,900 + -0.5 1,997,923 + -1.9 1,997,923 + -1.3 1,997,923 + +1.2 1,997,923 + -1.6 1,997,923 + +0.2 1,997,923 + -0.5 1,997,923 + +1.4 1,997,923 + -1.3 1,997,923 + +1.2 1,997,924 - +1.1 1,997,924 - -0.0 1,997,924 - +0.4 1,997,924 - -0.8 1,997,924 - +1.2 1,997,924 - +1.2 1,997,924 - +1.8 1,997,924 - -0.5 1,997,924 - -1.0 1,997,924 - +0.2 1,997,924 - -0.0 1,998,000 - -0.0 1,998,033 + +0.1 1,998,034 - -1.0 1,998,034 - +0.9 1,998,047 + -0.0 1,998,047 + +0.5 1,998,047 + -0.6 1,998,047 + +0.5 1,998,047 + -0.0 1,998,048 - +0.5 1,998,048 - -0.8 1,998,048 - +1.3 1,998,048 - +1.5 1,998,048 - +1.4 1,998,048 - +1.8 1,998,048 - -0.0 1,998,048 - +1.9 1,998,048 - +0.0 1,998,141 + +1.5 1,998,141 + -0.6 1,998,142 - +0.5 1,998,142 - -1.6 1,998,290 - +1.3 1,998,323 - +1.1 1,998,435 - +3.0 1,998,435 - +2.4 1,999,007 + Pf1N1B4_1746 0.46 +0.1 1,999,145 + Pf1N1B4_1746 0.55 +2.0 1,999,154 + Pf1N1B4_1746 0.56 -0.4 1,999,154 + Pf1N1B4_1746 0.56 +0.7 1,999,155 - Pf1N1B4_1746 0.56 +0.6 1,999,254 + Pf1N1B4_1746 0.63 +1.5 1,999,255 - Pf1N1B4_1746 0.63 +1.5 1,999,255 - Pf1N1B4_1746 0.63 +0.1 1,999,255 - Pf1N1B4_1746 0.63 -0.0 1,999,255 - Pf1N1B4_1746 0.63 +0.1 1,999,256 + Pf1N1B4_1746 0.63 +1.7 1,999,360 + Pf1N1B4_1746 0.70 +0.8 1,999,481 + Pf1N1B4_1746 0.78 +0.1 1,999,481 + Pf1N1B4_1746 0.78 -0.7 1,999,481 + Pf1N1B4_1746 0.78 +0.2 1,999,481 + Pf1N1B4_1746 0.78 -0.1 1,999,482 - Pf1N1B4_1746 0.78 +1.2 1,999,639 + Pf1N1B4_1746 0.89 +0.5 1,999,639 + Pf1N1B4_1746 0.89 +2.8 1,999,639 + Pf1N1B4_1746 0.89 -0.8 1,999,640 - Pf1N1B4_1746 0.89 -1.5 1,999,640 - Pf1N1B4_1746 0.89 +0.5 1,999,640 - Pf1N1B4_1746 0.89 +1.9 1,999,640 - Pf1N1B4_1746 0.89 -1.9 1,999,767 - +2.4 1,999,813 + -1.0 1,999,814 - -0.5 1,999,816 + -0.5 1,999,816 + -0.8 1,999,817 - +1.0 1,999,833 + -0.5 1,999,833 + -0.5 1,999,834 - +2.4 1,999,834 - +1.0 1,999,834 - +1.3 1,999,844 + +1.4 1,999,986 + +0.2 2,000,129 + +1.5 2,000,129 + +1.9 2,000,130 - -0.5 2,000,187 + Pf1N1B4_1748 0.10 -0.5 2,000,187 + Pf1N1B4_1748 0.10 +0.5 2,000,187 + Pf1N1B4_1748 0.10 -1.3 2,000,187 + Pf1N1B4_1748 0.10 +2.0 2,000,188 - Pf1N1B4_1748 0.10 -1.6 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 +1.5 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -2.7 2,000,188 - +0.5 2,000,188 - Pf1N1B4_1748 0.10 -0.0 2,000,188 - Pf1N1B4_1748 0.10 -1.4 2,000,188 - Pf1N1B4_1748 0.10 -2.5 2,000,189 + Pf1N1B4_1748 0.11 +0.7 2,000,189 + Pf1N1B4_1748 0.11 +1.2 2,000,189 + Pf1N1B4_1748 0.11 -1.5 2,000,189 + Pf1N1B4_1748 0.11 +1.9 2,000,190 - Pf1N1B4_1748 0.11 +1.5 2,000,457 - Pf1N1B4_1748 0.53 -1.3 2,000,472 - Pf1N1B4_1748 0.55 -2.3 2,000,578 + Pf1N1B4_1748 0.72 -1.0 2,000,579 - Pf1N1B4_1748 0.72 -0.5 2,000,645 - Pf1N1B4_1748 0.82 -0.0 2,000,690 + Pf1N1B4_1748 0.89 -0.0 2,000,690 + Pf1N1B4_1748 0.89 +2.5 2,000,690 + Pf1N1B4_1748 0.89 -1.0 2,000,691 - Pf1N1B4_1748 0.89 -1.1 2,000,691 - Pf1N1B4_1748 0.89 -1.6 2,000,691 - Pf1N1B4_1748 0.89 -1.9 2,000,691 - Pf1N1B4_1748 0.89 +0.7 2,000,691 - Pf1N1B4_1748 0.89 -1.0 2,000,691 - Pf1N1B4_1748 0.89 -2.3 2,000,758 + -1.6 2,000,758 + -0.5 2,000,759 - -0.0 2,000,761 + -1.6 2,000,761 + +1.1 2,000,761 + -0.8 2,000,761 + -1.6 2,000,762 - -0.0 2,000,762 - -1.5 2,000,762 - +0.5 2,000,762 - -0.0 2,000,762 - -1.8 2,000,762 - +0.3 2,000,762 - -1.0 2,000,762 - -0.2 2,000,762 - -0.5 2,000,762 - -0.0 2,000,762 - -2.5 2,000,762 - -0.5 2,000,762 - +0.4 2,000,762 - +0.2
Or see this region's nucleotide sequence