Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1732 and Pf1N1B4_1733 are separated by 148 nucleotides Pf1N1B4_1733 and Pf1N1B4_1734 are separated by 180 nucleotides
Pf1N1B4_1732 - response regulator, at 1,979,828 to 1,981,042
_1732
Pf1N1B4_1733 - 5-aminovalerate aminotransferase (EC 2.6.1.48) / Gamma-aminobutyrate:alpha-ketoglutarate aminotransferase (EC 2.6.1.19), at 1,981,191 to 1,982,468
_1733
Pf1N1B4_1734 - Succinate-semialdehyde dehydrogenase [NAD(P)+] (EC 1.2.1.16), at 1,982,649 to 1,984,091
_1734
Position (kb)
1981
1982
1983 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1980.192 kb on + strand, within Pf1N1B4_1732 at 1980.193 kb on - strand, within Pf1N1B4_1732 at 1980.240 kb on + strand, within Pf1N1B4_1732 at 1980.283 kb on - strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.451 kb on + strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.452 kb on - strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.517 kb on + strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.518 kb on - strand, within Pf1N1B4_1732 at 1980.588 kb on + strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.589 kb on - strand, within Pf1N1B4_1732 at 1980.711 kb on + strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.797 kb on - strand, within Pf1N1B4_1732 at 1980.802 kb on + strand, within Pf1N1B4_1732 at 1980.802 kb on + strand, within Pf1N1B4_1732 at 1980.876 kb on + strand, within Pf1N1B4_1732 at 1980.876 kb on + strand, within Pf1N1B4_1732 at 1980.877 kb on - strand, within Pf1N1B4_1732 at 1980.877 kb on - strand, within Pf1N1B4_1732 at 1981.025 kb on + strand at 1981.072 kb on + strand at 1981.073 kb on - strand at 1981.073 kb on - strand at 1981.093 kb on + strand at 1981.094 kb on - strand at 1981.128 kb on + strand at 1981.128 kb on + strand at 1981.128 kb on + strand at 1981.129 kb on - strand at 1981.270 kb on - strand at 1981.289 kb on - strand at 1981.289 kb on - strand at 1981.311 kb on - strand at 1981.441 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.573 kb on + strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.574 kb on - strand, within Pf1N1B4_1733 at 1981.601 kb on + strand, within Pf1N1B4_1733 at 1981.612 kb on + strand, within Pf1N1B4_1733 at 1981.612 kb on + strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.613 kb on - strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.743 kb on + strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.744 kb on - strand, within Pf1N1B4_1733 at 1981.761 kb on + strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.762 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1981.823 kb on - strand, within Pf1N1B4_1733 at 1982.015 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.053 kb on + strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.210 kb on - strand, within Pf1N1B4_1733 at 1982.218 kb on + strand, within Pf1N1B4_1733 at 1982.219 kb on - strand, within Pf1N1B4_1733 at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.468 kb on + strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.469 kb on - strand at 1982.531 kb on + strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.532 kb on - strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.650 kb on + strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.651 kb on - strand at 1982.668 kb on + strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.669 kb on - strand at 1982.683 kb on + strand at 1982.683 kb on + strand at 1982.698 kb on + strand at 1982.699 kb on - strand at 1982.699 kb on - strand at 1982.795 kb on - strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.842 kb on + strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.843 kb on - strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.848 kb on + strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1982.849 kb on - strand, within Pf1N1B4_1734 at 1983.187 kb on + strand, within Pf1N1B4_1734 at 1983.205 kb on + strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734 at 1983.206 kb on - strand, within Pf1N1B4_1734
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,980,192 + Pf1N1B4_1732 0.30 -1.8 1,980,193 - Pf1N1B4_1732 0.30 -1.3 1,980,240 + Pf1N1B4_1732 0.34 +0.7 1,980,283 - Pf1N1B4_1732 0.37 -0.2 1,980,451 + Pf1N1B4_1732 0.51 -0.1 1,980,451 + Pf1N1B4_1732 0.51 -0.5 1,980,452 - Pf1N1B4_1732 0.51 -0.5 1,980,452 - Pf1N1B4_1732 0.51 -0.0 1,980,517 + Pf1N1B4_1732 0.57 +0.2 1,980,517 + Pf1N1B4_1732 0.57 -1.5 1,980,518 - Pf1N1B4_1732 0.57 +0.9 1,980,518 - Pf1N1B4_1732 0.57 +1.5 1,980,588 + Pf1N1B4_1732 0.63 -0.4 1,980,589 - Pf1N1B4_1732 0.63 +0.4 1,980,589 - Pf1N1B4_1732 0.63 +1.7 1,980,589 - Pf1N1B4_1732 0.63 +0.5 1,980,589 - Pf1N1B4_1732 0.63 -0.7 1,980,711 + Pf1N1B4_1732 0.73 -0.5 1,980,797 - Pf1N1B4_1732 0.80 -0.8 1,980,797 - Pf1N1B4_1732 0.80 +1.5 1,980,797 - Pf1N1B4_1732 0.80 +2.1 1,980,802 + Pf1N1B4_1732 0.80 +0.4 1,980,802 + Pf1N1B4_1732 0.80 +0.2 1,980,876 + Pf1N1B4_1732 0.86 -0.6 1,980,876 + Pf1N1B4_1732 0.86 +1.9 1,980,877 - Pf1N1B4_1732 0.86 -0.7 1,980,877 - Pf1N1B4_1732 0.86 -0.5 1,981,025 + +3.5 1,981,072 + +1.7 1,981,073 - -0.0 1,981,073 - -0.0 1,981,093 + -0.6 1,981,094 - -0.6 1,981,128 + -0.5 1,981,128 + +0.7 1,981,128 + -1.3 1,981,129 - -1.5 1,981,270 - -1.2 1,981,289 - -0.0 1,981,289 - +1.0 1,981,311 - +1.9 1,981,441 + Pf1N1B4_1733 0.20 -1.5 1,981,573 + Pf1N1B4_1733 0.30 +1.0 1,981,573 + Pf1N1B4_1733 0.30 -1.6 1,981,573 + Pf1N1B4_1733 0.30 +0.2 1,981,574 - Pf1N1B4_1733 0.30 +1.0 1,981,574 - Pf1N1B4_1733 0.30 +1.1 1,981,574 - Pf1N1B4_1733 0.30 -1.4 1,981,574 - Pf1N1B4_1733 0.30 +0.5 1,981,574 - Pf1N1B4_1733 0.30 -1.5 1,981,601 + Pf1N1B4_1733 0.32 -1.4 1,981,612 + Pf1N1B4_1733 0.33 -0.8 1,981,612 + Pf1N1B4_1733 0.33 +2.2 1,981,613 - Pf1N1B4_1733 0.33 -1.0 1,981,613 - Pf1N1B4_1733 0.33 -1.5 1,981,613 - Pf1N1B4_1733 0.33 +1.3 1,981,613 - Pf1N1B4_1733 0.33 +0.2 1,981,613 - Pf1N1B4_1733 0.33 -1.9 1,981,613 - Pf1N1B4_1733 0.33 -0.3 1,981,743 + Pf1N1B4_1733 0.43 -1.0 1,981,743 + Pf1N1B4_1733 0.43 +1.5 1,981,744 - Pf1N1B4_1733 0.43 +0.0 1,981,744 - Pf1N1B4_1733 0.43 -1.3 1,981,744 - Pf1N1B4_1733 0.43 -0.0 1,981,744 - Pf1N1B4_1733 0.43 +1.5 1,981,761 + Pf1N1B4_1733 0.45 -0.1 1,981,762 - Pf1N1B4_1733 0.45 -2.9 1,981,762 - Pf1N1B4_1733 0.45 -1.5 1,981,762 - Pf1N1B4_1733 0.45 -0.0 1,981,762 - Pf1N1B4_1733 0.45 -1.7 1,981,762 - Pf1N1B4_1733 0.45 -2.0 1,981,762 - Pf1N1B4_1733 0.45 -1.9 1,981,762 - Pf1N1B4_1733 0.45 -0.2 1,981,823 - Pf1N1B4_1733 0.49 -0.8 1,981,823 - Pf1N1B4_1733 0.49 -1.5 1,982,015 + Pf1N1B4_1733 0.64 +0.8 1,982,053 + Pf1N1B4_1733 0.67 -0.4 1,982,053 + Pf1N1B4_1733 0.67 +1.5 1,982,210 - Pf1N1B4_1733 0.80 -1.9 1,982,210 - Pf1N1B4_1733 0.80 -0.8 1,982,210 - Pf1N1B4_1733 0.80 -1.8 1,982,210 - Pf1N1B4_1733 0.80 -1.6 1,982,218 + Pf1N1B4_1733 0.80 -0.5 1,982,219 - Pf1N1B4_1733 0.80 +0.3 1,982,468 + -0.8 1,982,468 + -1.0 1,982,468 + +0.1 1,982,468 + +1.5 1,982,468 + -0.6 1,982,468 + +0.1 1,982,469 - -0.3 1,982,469 - +0.5 1,982,469 - -0.2 1,982,469 - +1.8 1,982,469 - +1.4 1,982,469 - -1.0 1,982,531 + +0.4 1,982,532 - +0.1 1,982,532 - +1.5 1,982,532 - +1.5 1,982,650 + +1.5 1,982,650 + +2.5 1,982,650 + +0.9 1,982,650 + -0.0 1,982,650 + -0.8 1,982,650 + -1.2 1,982,650 + +0.5 1,982,651 - +0.2 1,982,651 - -1.0 1,982,651 - -2.5 1,982,668 + -0.0 1,982,669 - -0.6 1,982,669 - +1.0 1,982,669 - -0.3 1,982,669 - +1.9 1,982,683 + +0.5 1,982,683 + +1.4 1,982,698 + -1.5 1,982,699 - +0.1 1,982,699 - +0.1 1,982,795 - Pf1N1B4_1734 0.10 -0.5 1,982,842 + Pf1N1B4_1734 0.13 -0.3 1,982,842 + Pf1N1B4_1734 0.13 +0.5 1,982,842 + Pf1N1B4_1734 0.13 +1.5 1,982,842 + Pf1N1B4_1734 0.13 +2.7 1,982,842 + Pf1N1B4_1734 0.13 -1.3 1,982,843 - Pf1N1B4_1734 0.13 -0.0 1,982,843 - Pf1N1B4_1734 0.13 -0.8 1,982,843 - Pf1N1B4_1734 0.13 -1.0 1,982,843 - Pf1N1B4_1734 0.13 -0.0 1,982,843 - Pf1N1B4_1734 0.13 -0.3 1,982,843 - Pf1N1B4_1734 0.13 -0.8 1,982,843 - Pf1N1B4_1734 0.13 +3.5 1,982,843 - Pf1N1B4_1734 0.13 -1.3 1,982,843 - Pf1N1B4_1734 0.13 -1.3 1,982,843 - Pf1N1B4_1734 0.13 -2.5 1,982,843 - Pf1N1B4_1734 0.13 +1.1 1,982,843 - Pf1N1B4_1734 0.13 -0.0 1,982,848 + Pf1N1B4_1734 0.14 +1.2 1,982,848 + Pf1N1B4_1734 0.14 +1.0 1,982,848 + Pf1N1B4_1734 0.14 -0.9 1,982,848 + Pf1N1B4_1734 0.14 -0.5 1,982,848 + Pf1N1B4_1734 0.14 -1.0 1,982,848 + Pf1N1B4_1734 0.14 +2.7 1,982,849 - Pf1N1B4_1734 0.14 -1.5 1,982,849 - Pf1N1B4_1734 0.14 -0.0 1,982,849 - Pf1N1B4_1734 0.14 +1.1 1,983,187 + Pf1N1B4_1734 0.37 -0.5 1,983,205 + Pf1N1B4_1734 0.39 -0.0 1,983,206 - Pf1N1B4_1734 0.39 +0.9 1,983,206 - Pf1N1B4_1734 0.39 +2.7 1,983,206 - Pf1N1B4_1734 0.39 -2.2
Or see this region's nucleotide sequence