Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1591 and Pf1N1B4_1592 are separated by 118 nucleotides Pf1N1B4_1592 and Pf1N1B4_1593 are separated by 33 nucleotides Pf1N1B4_1593 and Pf1N1B4_1594 are separated by 291 nucleotides
Pf1N1B4_1591 - Formiminoglutamic iminohydrolase (EC 3.5.3.13) (from data) , at 1,832,279 to 1,833,643
_1591
Pf1N1B4_1592 - Outer membrane lipoprotein Blc, at 1,833,762 to 1,834,331
_1592
Pf1N1B4_1593 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 1,834,365 to 1,834,619
_1593
Pf1N1B4_1594 - Methyl-accepting chemotaxis protein, at 1,834,911 to 1,836,791
_1594
Position (kb)
1834
1835 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.467 kb on + strand, within Pf1N1B4_1591 at 1833.601 kb on + strand at 1833.602 kb on - strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.745 kb on + strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.746 kb on - strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.763 kb on + strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.764 kb on - strand at 1833.789 kb on - strand at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.940 kb on + strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.941 kb on - strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.955 kb on + strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.956 kb on - strand, within Pf1N1B4_1592 at 1833.967 kb on + strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1833.968 kb on - strand, within Pf1N1B4_1592 at 1834.057 kb on + strand at 1834.144 kb on + strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.145 kb on - strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.180 kb on + strand, within Pf1N1B4_1592 at 1834.181 kb on - strand, within Pf1N1B4_1592 at 1834.266 kb on + strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.267 kb on - strand, within Pf1N1B4_1592 at 1834.312 kb on - strand at 1834.333 kb on - strand at 1834.355 kb on + strand at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.411 kb on + strand, within Pf1N1B4_1593 at 1834.412 kb on - strand, within Pf1N1B4_1593 at 1834.425 kb on - strand, within Pf1N1B4_1593 at 1834.604 kb on + strand at 1834.604 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.624 kb on + strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.625 kb on - strand at 1834.669 kb on + strand at 1834.669 kb on + strand at 1834.669 kb on + strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.670 kb on - strand at 1834.709 kb on + strand at 1834.741 kb on + strand at 1834.741 kb on + strand at 1834.770 kb on + strand at 1834.771 kb on - strand at 1834.798 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.824 kb on + strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.825 kb on - strand at 1834.835 kb on + strand at 1834.835 kb on + strand at 1834.836 kb on - strand at 1834.897 kb on + strand at 1834.897 kb on + strand at 1834.897 kb on + strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1834.898 kb on - strand at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.178 kb on + strand, within Pf1N1B4_1594 at 1835.180 kb on + strand, within Pf1N1B4_1594 at 1835.202 kb on + strand, within Pf1N1B4_1594 at 1835.202 kb on + strand, within Pf1N1B4_1594 at 1835.203 kb on - strand, within Pf1N1B4_1594 at 1835.203 kb on - strand, within Pf1N1B4_1594 at 1835.262 kb on + strand, within Pf1N1B4_1594 at 1835.262 kb on + strand, within Pf1N1B4_1594 at 1835.263 kb on - strand, within Pf1N1B4_1594 at 1835.286 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.331 kb on + strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.332 kb on - strand, within Pf1N1B4_1594 at 1835.363 kb on + strand, within Pf1N1B4_1594 at 1835.486 kb on + strand, within Pf1N1B4_1594 at 1835.486 kb on + strand, within Pf1N1B4_1594 at 1835.559 kb on + strand, within Pf1N1B4_1594
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,833,467 + Pf1N1B4_1591 0.87 +1.4 1,833,467 + Pf1N1B4_1591 0.87 -0.8 1,833,601 + -1.5 1,833,602 - -0.5 1,833,745 + -0.2 1,833,745 + -1.0 1,833,745 + -0.1 1,833,745 + +1.3 1,833,745 + -1.1 1,833,746 - -0.1 1,833,746 - -0.4 1,833,746 - -0.8 1,833,746 - +1.2 1,833,746 - +1.4 1,833,746 - -2.7 1,833,746 - -1.1 1,833,746 - +1.2 1,833,763 + +0.4 1,833,763 + +1.6 1,833,763 + -0.1 1,833,764 - -1.3 1,833,764 - -2.9 1,833,764 - -0.1 1,833,764 - -0.6 1,833,789 - -0.1 1,833,940 + Pf1N1B4_1592 0.31 +1.0 1,833,940 + Pf1N1B4_1592 0.31 +1.3 1,833,940 + Pf1N1B4_1592 0.31 -1.6 1,833,940 + Pf1N1B4_1592 0.31 +0.5 1,833,941 - Pf1N1B4_1592 0.31 +0.2 1,833,941 - Pf1N1B4_1592 0.31 -2.2 1,833,941 - Pf1N1B4_1592 0.31 -0.6 1,833,941 - Pf1N1B4_1592 0.31 +0.5 1,833,955 + Pf1N1B4_1592 0.34 -0.8 1,833,955 + Pf1N1B4_1592 0.34 -0.2 1,833,955 + Pf1N1B4_1592 0.34 +0.4 1,833,956 - Pf1N1B4_1592 0.34 -0.1 1,833,956 - Pf1N1B4_1592 0.34 +0.1 1,833,956 - Pf1N1B4_1592 0.34 -0.5 1,833,956 - Pf1N1B4_1592 0.34 -1.9 1,833,967 + Pf1N1B4_1592 0.36 +0.5 1,833,968 - Pf1N1B4_1592 0.36 +1.4 1,833,968 - Pf1N1B4_1592 0.36 +0.5 1,833,968 - Pf1N1B4_1592 0.36 +0.5 1,834,057 + +0.5 1,834,144 + Pf1N1B4_1592 0.67 +1.7 1,834,145 - Pf1N1B4_1592 0.67 +2.3 1,834,145 - Pf1N1B4_1592 0.67 -1.8 1,834,180 + Pf1N1B4_1592 0.73 +0.4 1,834,180 + Pf1N1B4_1592 0.73 +1.2 1,834,180 + Pf1N1B4_1592 0.73 -1.1 1,834,181 - Pf1N1B4_1592 0.74 +1.5 1,834,266 + Pf1N1B4_1592 0.88 -0.9 1,834,267 - Pf1N1B4_1592 0.89 +0.7 1,834,267 - Pf1N1B4_1592 0.89 +0.5 1,834,267 - Pf1N1B4_1592 0.89 -0.8 1,834,312 - -0.5 1,834,333 - -1.6 1,834,355 + -0.6 1,834,411 + Pf1N1B4_1593 0.18 -1.6 1,834,411 + Pf1N1B4_1593 0.18 +0.8 1,834,411 + Pf1N1B4_1593 0.18 -1.8 1,834,412 - Pf1N1B4_1593 0.18 +0.2 1,834,425 - Pf1N1B4_1593 0.24 +1.5 1,834,604 + +0.3 1,834,604 + -0.8 1,834,624 + -1.6 1,834,624 + -1.5 1,834,624 + -0.5 1,834,625 - -0.5 1,834,625 - -0.1 1,834,625 - -0.1 1,834,625 - +0.1 1,834,625 - +1.8 1,834,669 + -1.5 1,834,669 + -0.9 1,834,669 + +0.1 1,834,670 - -0.8 1,834,670 - +2.5 1,834,670 - -1.3 1,834,670 - +0.2 1,834,709 + +0.5 1,834,741 + +0.8 1,834,741 + -2.4 1,834,770 + -1.3 1,834,771 - -0.5 1,834,798 + -2.9 1,834,824 + -0.3 1,834,824 + +0.2 1,834,824 + -0.8 1,834,824 + -0.2 1,834,824 + -0.3 1,834,824 + +0.5 1,834,824 + -1.5 1,834,824 + -0.1 1,834,824 + -0.3 1,834,825 - -1.2 1,834,825 - -0.9 1,834,825 - -2.2 1,834,825 - -1.1 1,834,825 - -0.7 1,834,825 - +0.9 1,834,825 - -0.1 1,834,825 - -0.3 1,834,835 + -0.1 1,834,835 + +0.5 1,834,836 - -1.3 1,834,897 + -0.3 1,834,897 + -2.2 1,834,897 + -0.9 1,834,898 - +0.1 1,834,898 - -0.3 1,834,898 - +1.2 1,834,898 - -0.4 1,834,898 - +1.0 1,834,898 - +0.5 1,835,178 + Pf1N1B4_1594 0.14 +1.0 1,835,178 + Pf1N1B4_1594 0.14 +0.2 1,835,178 + Pf1N1B4_1594 0.14 -0.3 1,835,180 + Pf1N1B4_1594 0.14 -0.1 1,835,202 + Pf1N1B4_1594 0.15 -1.9 1,835,202 + Pf1N1B4_1594 0.15 -1.1 1,835,203 - Pf1N1B4_1594 0.16 -0.1 1,835,203 - Pf1N1B4_1594 0.16 -0.5 1,835,262 + Pf1N1B4_1594 0.19 -1.3 1,835,262 + Pf1N1B4_1594 0.19 -1.3 1,835,263 - Pf1N1B4_1594 0.19 +2.3 1,835,286 + Pf1N1B4_1594 0.20 +0.5 1,835,331 + Pf1N1B4_1594 0.22 +0.9 1,835,331 + Pf1N1B4_1594 0.22 +1.7 1,835,331 + Pf1N1B4_1594 0.22 +3.1 1,835,331 + Pf1N1B4_1594 0.22 +1.9 1,835,331 + Pf1N1B4_1594 0.22 +1.0 1,835,331 + Pf1N1B4_1594 0.22 -2.1 1,835,332 - Pf1N1B4_1594 0.22 -0.8 1,835,332 - Pf1N1B4_1594 0.22 -0.4 1,835,332 - Pf1N1B4_1594 0.22 +0.7 1,835,332 - Pf1N1B4_1594 0.22 +1.3 1,835,363 + Pf1N1B4_1594 0.24 -0.2 1,835,486 + Pf1N1B4_1594 0.31 -1.6 1,835,486 + Pf1N1B4_1594 0.31 -0.1 1,835,559 + Pf1N1B4_1594 0.34 -1.2
Or see this region's nucleotide sequence