Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1506 and Pf1N1B4_1507 are separated by 164 nucleotides Pf1N1B4_1507 and Pf1N1B4_1508 overlap by 4 nucleotides Pf1N1B4_1508 and Pf1N1B4_1509 overlap by 20 nucleotides
Pf1N1B4_1506 - FIGfam138462: Acyl-CoA synthetase, AMP-(fatty) acid ligase / (3R)-hydroxymyristoyl-[ACP] dehydratase (EC 4.2.1.-), at 1,738,249 to 1,739,550
_1506
Pf1N1B4_1507 - FIG143263: Glycosyl transferase, at 1,739,715 to 1,740,449
_1507
Pf1N1B4_1508 - Lysophospholipid acyltransferase, at 1,740,446 to 1,741,393
_1508
Pf1N1B4_1509 - Putative histidine ammonia-lyase protein, at 1,741,374 to 1,742,918
_1509
Position (kb)
1739
1740
1741 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3 at 1738.771 kb on - strand, within Pf1N1B4_1506 at 1738.783 kb on + strand, within Pf1N1B4_1506 at 1738.783 kb on + strand, within Pf1N1B4_1506 at 1738.784 kb on - strand, within Pf1N1B4_1506 at 1738.897 kb on - strand, within Pf1N1B4_1506 at 1738.897 kb on - strand, within Pf1N1B4_1506 at 1738.988 kb on + strand, within Pf1N1B4_1506 at 1739.004 kb on - strand, within Pf1N1B4_1506 at 1739.056 kb on - strand, within Pf1N1B4_1506 at 1739.136 kb on - strand, within Pf1N1B4_1506 at 1739.282 kb on - strand, within Pf1N1B4_1506 at 1739.282 kb on - strand, within Pf1N1B4_1506 at 1739.479 kb on + strand at 1739.480 kb on - strand at 1739.480 kb on - strand at 1739.480 kb on - strand at 1739.529 kb on - strand at 1739.566 kb on - strand at 1739.624 kb on - strand at 1739.651 kb on - strand at 1739.651 kb on - strand at 1739.651 kb on - strand at 1739.721 kb on - strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.745 kb on + strand at 1739.746 kb on - strand at 1739.746 kb on - strand at 1739.880 kb on + strand, within Pf1N1B4_1507 at 1739.880 kb on + strand, within Pf1N1B4_1507 at 1740.012 kb on - strand, within Pf1N1B4_1507 at 1740.073 kb on - strand, within Pf1N1B4_1507 at 1740.101 kb on - strand, within Pf1N1B4_1507 at 1740.101 kb on - strand, within Pf1N1B4_1507 at 1740.102 kb on + strand at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.103 kb on - strand, within Pf1N1B4_1507 at 1740.187 kb on - strand, within Pf1N1B4_1507 at 1740.240 kb on - strand, within Pf1N1B4_1507 at 1740.240 kb on - strand, within Pf1N1B4_1507 at 1740.364 kb on + strand, within Pf1N1B4_1507 at 1740.364 kb on + strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand, within Pf1N1B4_1507 at 1740.365 kb on - strand at 1740.569 kb on + strand, within Pf1N1B4_1508 at 1740.585 kb on - strand, within Pf1N1B4_1508 at 1740.585 kb on - strand, within Pf1N1B4_1508 at 1740.802 kb on + strand, within Pf1N1B4_1508 at 1740.802 kb on + strand, within Pf1N1B4_1508 at 1740.803 kb on - strand, within Pf1N1B4_1508 at 1740.803 kb on - strand, within Pf1N1B4_1508 at 1740.834 kb on + strand at 1741.320 kb on - strand at 1741.356 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,738,771 - Pf1N1B4_1506 0.40 +0.6 1,738,783 + Pf1N1B4_1506 0.41 +2.0 1,738,783 + Pf1N1B4_1506 0.41 -0.1 1,738,784 - Pf1N1B4_1506 0.41 -2.2 1,738,897 - Pf1N1B4_1506 0.50 -0.9 1,738,897 - Pf1N1B4_1506 0.50 -2.1 1,738,988 + Pf1N1B4_1506 0.57 +1.2 1,739,004 - Pf1N1B4_1506 0.58 -2.2 1,739,056 - Pf1N1B4_1506 0.62 -0.2 1,739,136 - Pf1N1B4_1506 0.68 -1.3 1,739,282 - Pf1N1B4_1506 0.79 -4.5 1,739,282 - Pf1N1B4_1506 0.79 -2.4 1,739,479 + +1.9 1,739,480 - -1.1 1,739,480 - +0.9 1,739,480 - -2.1 1,739,529 - -1.8 1,739,566 - -1.3 1,739,624 - -1.5 1,739,651 - +1.1 1,739,651 - -0.7 1,739,651 - -2.9 1,739,721 - -0.9 1,739,745 + +0.1 1,739,745 + +2.5 1,739,745 + +1.8 1,739,745 + -1.0 1,739,746 - +0.3 1,739,746 - -0.1 1,739,880 + Pf1N1B4_1507 0.22 -1.5 1,739,880 + Pf1N1B4_1507 0.22 -1.8 1,740,012 - Pf1N1B4_1507 0.40 -1.8 1,740,073 - Pf1N1B4_1507 0.49 -0.3 1,740,101 - Pf1N1B4_1507 0.53 -2.9 1,740,101 - Pf1N1B4_1507 0.53 -0.5 1,740,102 + +0.5 1,740,103 - Pf1N1B4_1507 0.53 -1.0 1,740,103 - Pf1N1B4_1507 0.53 -0.5 1,740,103 - Pf1N1B4_1507 0.53 -0.8 1,740,187 - Pf1N1B4_1507 0.64 -2.6 1,740,240 - Pf1N1B4_1507 0.71 -1.7 1,740,240 - Pf1N1B4_1507 0.71 -1.3 1,740,364 + Pf1N1B4_1507 0.88 +1.7 1,740,364 + Pf1N1B4_1507 0.88 +0.5 1,740,365 - Pf1N1B4_1507 0.88 -0.8 1,740,365 - Pf1N1B4_1507 0.88 -1.8 1,740,365 - Pf1N1B4_1507 0.88 -3.7 1,740,365 - +3.1 1,740,569 + Pf1N1B4_1508 0.13 -1.1 1,740,585 - Pf1N1B4_1508 0.15 -1.1 1,740,585 - Pf1N1B4_1508 0.15 -0.3 1,740,802 + Pf1N1B4_1508 0.38 +0.9 1,740,802 + Pf1N1B4_1508 0.38 -1.8 1,740,803 - Pf1N1B4_1508 0.38 -2.5 1,740,803 - Pf1N1B4_1508 0.38 -2.5 1,740,834 + -0.1 1,741,320 - +0.3 1,741,356 - +0.2
Or see this region's nucleotide sequence