Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_1219 and Pf1N1B4_1220 are separated by 220 nucleotides Pf1N1B4_1220 and Pf1N1B4_1221 are separated by 156 nucleotides
Pf1N1B4_1219 - Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2), at 1,382,749 to 1,383,906
_1219
Pf1N1B4_1220 - 2-hydroxy-3-oxopropionate reductase (EC 1.1.1.60), at 1,384,127 to 1,385,002
_1220
Pf1N1B4_1221 - coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase, at 1,385,159 to 1,386,304
_1221
Position (kb)
1384
1385
1386 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1383.200 kb on + strand, within Pf1N1B4_1219 at 1383.203 kb on + strand, within Pf1N1B4_1219 at 1383.216 kb on - strand, within Pf1N1B4_1219 at 1383.229 kb on - strand, within Pf1N1B4_1219 at 1383.440 kb on - strand, within Pf1N1B4_1219 at 1383.456 kb on - strand, within Pf1N1B4_1219 at 1383.456 kb on - strand, within Pf1N1B4_1219 at 1383.497 kb on + strand, within Pf1N1B4_1219 at 1383.569 kb on + strand, within Pf1N1B4_1219 at 1383.569 kb on + strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.570 kb on - strand, within Pf1N1B4_1219 at 1383.599 kb on + strand, within Pf1N1B4_1219 at 1383.600 kb on - strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.772 kb on + strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.773 kb on - strand, within Pf1N1B4_1219 at 1383.806 kb on + strand at 1383.806 kb on + strand at 1383.895 kb on - strand at 1383.916 kb on + strand at 1383.916 kb on + strand at 1383.917 kb on - strand at 1383.923 kb on + strand at 1383.923 kb on + strand at 1383.924 kb on - strand at 1383.924 kb on - strand at 1383.927 kb on + strand at 1383.967 kb on - strand at 1384.023 kb on - strand at 1384.097 kb on - strand at 1384.128 kb on + strand at 1384.129 kb on - strand at 1384.129 kb on - strand at 1384.129 kb on - strand at 1384.285 kb on + strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.426 kb on - strand, within Pf1N1B4_1220 at 1384.665 kb on + strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.666 kb on - strand, within Pf1N1B4_1220 at 1384.732 kb on + strand, within Pf1N1B4_1220 at 1384.732 kb on + strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand, within Pf1N1B4_1220 at 1384.733 kb on - strand at 1384.987 kb on + strand at 1384.988 kb on - strand at 1384.988 kb on - strand at 1385.069 kb on - strand at 1385.102 kb on + strand at 1385.103 kb on - strand at 1385.103 kb on - strand at 1385.106 kb on + strand at 1385.136 kb on + strand at 1385.136 kb on + strand at 1385.136 kb on + strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.137 kb on - strand at 1385.174 kb on + strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.175 kb on - strand at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.354 kb on + strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.355 kb on - strand, within Pf1N1B4_1221 at 1385.441 kb on + strand, within Pf1N1B4_1221 at 1385.501 kb on - strand, within Pf1N1B4_1221 at 1385.533 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.732 kb on + strand, within Pf1N1B4_1221 at 1385.733 kb on - strand, within Pf1N1B4_1221 at 1385.762 kb on - strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.872 kb on + strand, within Pf1N1B4_1221 at 1385.873 kb on - strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.921 kb on + strand, within Pf1N1B4_1221 at 1385.922 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 1,383,200 + Pf1N1B4_1219 0.39 +1.4 1,383,203 + Pf1N1B4_1219 0.39 +2.1 1,383,216 - Pf1N1B4_1219 0.40 -0.2 1,383,229 - Pf1N1B4_1219 0.41 -0.4 1,383,440 - Pf1N1B4_1219 0.60 +1.4 1,383,456 - Pf1N1B4_1219 0.61 +0.1 1,383,456 - Pf1N1B4_1219 0.61 +0.3 1,383,497 + Pf1N1B4_1219 0.65 +0.8 1,383,569 + Pf1N1B4_1219 0.71 -1.8 1,383,569 + Pf1N1B4_1219 0.71 +0.4 1,383,570 - Pf1N1B4_1219 0.71 -0.2 1,383,570 - Pf1N1B4_1219 0.71 -1.9 1,383,570 - Pf1N1B4_1219 0.71 -0.2 1,383,570 - Pf1N1B4_1219 0.71 -1.8 1,383,570 - Pf1N1B4_1219 0.71 -1.2 1,383,570 - Pf1N1B4_1219 0.71 -1.2 1,383,570 - Pf1N1B4_1219 0.71 -1.2 1,383,570 - Pf1N1B4_1219 0.71 +0.6 1,383,570 - Pf1N1B4_1219 0.71 +0.4 1,383,570 - Pf1N1B4_1219 0.71 -0.9 1,383,570 - Pf1N1B4_1219 0.71 +1.4 1,383,570 - Pf1N1B4_1219 0.71 +1.9 1,383,599 + Pf1N1B4_1219 0.73 +0.5 1,383,600 - Pf1N1B4_1219 0.73 -0.2 1,383,772 + Pf1N1B4_1219 0.88 -1.6 1,383,772 + Pf1N1B4_1219 0.88 -0.6 1,383,772 + Pf1N1B4_1219 0.88 +0.3 1,383,773 - Pf1N1B4_1219 0.88 -0.2 1,383,773 - Pf1N1B4_1219 0.88 -1.8 1,383,773 - Pf1N1B4_1219 0.88 -0.8 1,383,773 - Pf1N1B4_1219 0.88 -0.5 1,383,773 - Pf1N1B4_1219 0.88 +1.7 1,383,773 - Pf1N1B4_1219 0.88 -0.4 1,383,773 - Pf1N1B4_1219 0.88 +0.4 1,383,773 - Pf1N1B4_1219 0.88 +0.3 1,383,773 - Pf1N1B4_1219 0.88 -1.4 1,383,773 - Pf1N1B4_1219 0.88 -1.8 1,383,773 - Pf1N1B4_1219 0.88 -0.2 1,383,806 + +0.7 1,383,806 + -1.4 1,383,895 - +1.2 1,383,916 + +0.4 1,383,916 + +0.8 1,383,917 - -2.6 1,383,923 + +0.3 1,383,923 + -1.6 1,383,924 - +0.4 1,383,924 - -0.8 1,383,927 + +1.4 1,383,967 - +0.4 1,384,023 - -1.2 1,384,097 - -1.2 1,384,128 + -0.9 1,384,129 - +0.1 1,384,129 - -1.4 1,384,129 - -1.1 1,384,285 + Pf1N1B4_1220 0.18 +0.4 1,384,426 - Pf1N1B4_1220 0.34 -0.7 1,384,426 - Pf1N1B4_1220 0.34 -1.5 1,384,426 - Pf1N1B4_1220 0.34 -1.4 1,384,665 + Pf1N1B4_1220 0.61 -1.6 1,384,666 - Pf1N1B4_1220 0.62 -1.8 1,384,666 - Pf1N1B4_1220 0.62 -0.4 1,384,666 - Pf1N1B4_1220 0.62 +2.4 1,384,732 + Pf1N1B4_1220 0.69 -1.6 1,384,732 + Pf1N1B4_1220 0.69 -1.4 1,384,733 - Pf1N1B4_1220 0.69 -2.1 1,384,733 - Pf1N1B4_1220 0.69 -1.3 1,384,733 - Pf1N1B4_1220 0.69 +1.4 1,384,733 - Pf1N1B4_1220 0.69 -1.4 1,384,733 - Pf1N1B4_1220 0.69 +1.1 1,384,733 - Pf1N1B4_1220 0.69 -1.6 1,384,733 - Pf1N1B4_1220 0.69 -1.8 1,384,733 - Pf1N1B4_1220 0.69 -0.2 1,384,733 - Pf1N1B4_1220 0.69 -2.7 1,384,733 - Pf1N1B4_1220 0.69 -0.1 1,384,733 - Pf1N1B4_1220 0.69 -0.0 1,384,733 - -0.9 1,384,987 + -0.6 1,384,988 - -0.2 1,384,988 - -0.6 1,385,069 - -1.8 1,385,102 + +0.4 1,385,103 - -0.0 1,385,103 - -1.2 1,385,106 + -0.4 1,385,136 + -0.6 1,385,136 + +1.4 1,385,136 + -0.6 1,385,137 - -0.6 1,385,137 - -1.4 1,385,137 - -1.8 1,385,137 - -0.6 1,385,174 + -1.2 1,385,175 - -1.4 1,385,175 - +0.4 1,385,175 - -0.4 1,385,175 - +0.4 1,385,175 - -1.8 1,385,175 - -1.5 1,385,354 + Pf1N1B4_1221 0.17 -0.4 1,385,354 + Pf1N1B4_1221 0.17 -2.8 1,385,355 - Pf1N1B4_1221 0.17 -0.9 1,385,355 - Pf1N1B4_1221 0.17 +0.4 1,385,355 - Pf1N1B4_1221 0.17 +0.8 1,385,355 - Pf1N1B4_1221 0.17 -0.6 1,385,355 - Pf1N1B4_1221 0.17 -0.3 1,385,441 + Pf1N1B4_1221 0.25 -1.4 1,385,501 - Pf1N1B4_1221 0.30 -0.6 1,385,533 + Pf1N1B4_1221 0.33 +2.4 1,385,732 + Pf1N1B4_1221 0.50 +0.4 1,385,732 + Pf1N1B4_1221 0.50 -1.2 1,385,732 + Pf1N1B4_1221 0.50 -0.8 1,385,733 - Pf1N1B4_1221 0.50 -0.9 1,385,762 - Pf1N1B4_1221 0.53 -0.6 1,385,872 + Pf1N1B4_1221 0.62 -0.2 1,385,872 + Pf1N1B4_1221 0.62 +0.7 1,385,873 - Pf1N1B4_1221 0.62 -2.6 1,385,921 + Pf1N1B4_1221 0.66 +1.4 1,385,921 + Pf1N1B4_1221 0.66 -0.2 1,385,921 + Pf1N1B4_1221 0.66 -0.2 1,385,921 + Pf1N1B4_1221 0.66 +1.0 1,385,922 - +0.8
Or see this region's nucleotide sequence