Experiment: Doxycycline hyclate 0.0001 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf1N1B4_104 and Pf1N1B4_105 overlap by 4 nucleotides Pf1N1B4_105 and Pf1N1B4_106 are separated by 3 nucleotides Pf1N1B4_106 and Pf1N1B4_107 are separated by 38 nucleotides Pf1N1B4_107 and Pf1N1B4_108 are separated by 243 nucleotides
Pf1N1B4_104 - Sensor protein PhoQ (EC 2.7.13.3), at 110,365 to 111,678
_104
Pf1N1B4_105 - Two-component system response regulator QseB, at 111,675 to 112,343
_105
Pf1N1B4_106 - Phosphonate ABC transporter phosphate-binding periplasmic component (TC 3.A.1.9.1), at 112,347 to 112,628
_106
Pf1N1B4_107 - FIG00857858: hypothetical protein, at 112,667 to 112,975
_107
Pf1N1B4_108 - FIG00954456: hypothetical protein, at 113,219 to 114,298
_108
Position (kb)
112
113 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 111.435 kb on + strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.436 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.441 kb on - strand, within Pf1N1B4_104 at 111.996 kb on + strand, within Pf1N1B4_105 at 112.232 kb on - strand, within Pf1N1B4_105 at 112.364 kb on + strand at 112.364 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.368 kb on + strand at 112.369 kb on - strand at 112.395 kb on + strand, within Pf1N1B4_106 at 112.409 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand, within Pf1N1B4_106 at 112.438 kb on + strand at 112.438 kb on + strand, within Pf1N1B4_106 at 112.444 kb on + strand, within Pf1N1B4_106 at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.668 kb on + strand at 112.761 kb on + strand, within Pf1N1B4_107 at 112.762 kb on - strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.767 kb on + strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.768 kb on - strand, within Pf1N1B4_107 at 112.777 kb on - strand at 112.864 kb on + strand, within Pf1N1B4_107 at 112.865 kb on - strand, within Pf1N1B4_107 at 112.988 kb on + strand at 113.006 kb on + strand at 113.006 kb on + strand at 113.007 kb on - strand at 113.007 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.016 kb on - strand at 113.020 kb on + strand at 113.020 kb on + strand at 113.035 kb on - strand at 113.087 kb on - strand at 113.126 kb on + strand at 113.127 kb on - strand at 113.128 kb on + strand at 113.154 kb on + strand at 113.154 kb on + strand at 113.155 kb on - strand at 113.510 kb on + strand, within Pf1N1B4_108 at 113.510 kb on + strand, within Pf1N1B4_108
Per-strain Table
Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml remove 111,435 + Pf1N1B4_104 0.81 +0.7 111,436 - Pf1N1B4_104 0.82 -0.5 111,436 - Pf1N1B4_104 0.82 +0.1 111,436 - Pf1N1B4_104 0.82 -1.5 111,441 - Pf1N1B4_104 0.82 -3.2 111,441 - Pf1N1B4_104 0.82 -0.9 111,996 + Pf1N1B4_105 0.48 -1.5 112,232 - Pf1N1B4_105 0.83 -1.9 112,364 + -0.1 112,364 + +1.1 112,368 + -1.7 112,368 + +0.1 112,368 + +1.2 112,369 - +1.1 112,395 + Pf1N1B4_106 0.17 +0.4 112,409 + Pf1N1B4_106 0.22 +1.1 112,438 + Pf1N1B4_106 0.32 +1.4 112,438 + +1.1 112,438 + Pf1N1B4_106 0.32 +1.7 112,444 + Pf1N1B4_106 0.34 +1.6 112,668 + +0.1 112,668 + +1.1 112,668 + -1.7 112,668 + -0.9 112,668 + -1.5 112,761 + Pf1N1B4_107 0.30 -1.9 112,762 - Pf1N1B4_107 0.31 +1.7 112,767 + Pf1N1B4_107 0.32 -0.7 112,767 + Pf1N1B4_107 0.32 +0.1 112,767 + Pf1N1B4_107 0.32 +1.8 112,768 - Pf1N1B4_107 0.33 -0.2 112,768 - Pf1N1B4_107 0.33 -1.2 112,768 - Pf1N1B4_107 0.33 -0.2 112,768 - Pf1N1B4_107 0.33 +3.7 112,777 - +1.1 112,864 + Pf1N1B4_107 0.64 -1.1 112,865 - Pf1N1B4_107 0.64 -1.9 112,988 + +0.1 113,006 + -0.9 113,006 + -1.2 113,007 - -0.9 113,007 - -1.2 113,016 - -0.5 113,016 - -1.2 113,016 - +0.7 113,020 + +0.1 113,020 + -0.7 113,035 - -0.9 113,087 - -1.5 113,126 + -1.3 113,127 - -0.5 113,128 + -0.1 113,154 + +1.4 113,154 + +1.2 113,155 - +0.1 113,510 + Pf1N1B4_108 0.27 +1.1 113,510 + Pf1N1B4_108 0.27 -0.5
Or see this region's nucleotide sequence