Strain Fitness in Pseudomonas fluorescens FW300-N1B4 around Pf1N1B4_100

Experiment: Doxycycline hyclate 0.0001 mg/ml

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf1N1B4_99 and Pf1N1B4_100 are separated by 308 nucleotidesPf1N1B4_100 and Pf1N1B4_101 are separated by 64 nucleotidesPf1N1B4_101 and Pf1N1B4_102 are separated by 374 nucleotides Pf1N1B4_99 - Purine nucleoside permease, at 104,512 to 105,546 _99 Pf1N1B4_100 - FIG00953437: hypothetical protein, at 105,855 to 106,082 _100 Pf1N1B4_101 - hypothetical protein, at 106,147 to 106,311 _101 Pf1N1B4_102 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 106,686 to 108,446 _102 Position (kb) 105 106 107Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 104.902 kb on + strand, within Pf1N1B4_99at 104.902 kb on + strand, within Pf1N1B4_99at 104.903 kb on - strand, within Pf1N1B4_99at 104.903 kb on - strandat 105.020 kb on - strand, within Pf1N1B4_99at 105.192 kb on + strand, within Pf1N1B4_99at 105.192 kb on + strand, within Pf1N1B4_99at 105.255 kb on + strand, within Pf1N1B4_99at 105.268 kb on + strand, within Pf1N1B4_99at 105.268 kb on + strand, within Pf1N1B4_99at 105.269 kb on - strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.301 kb on + strand, within Pf1N1B4_99at 105.302 kb on - strandat 105.302 kb on - strand, within Pf1N1B4_99at 105.428 kb on - strandat 105.657 kb on + strandat 105.657 kb on + strandat 105.657 kb on + strandat 105.658 kb on - strandat 105.791 kb on - strandat 105.828 kb on - strandat 105.828 kb on - strandat 105.829 kb on + strandat 105.830 kb on - strandat 105.830 kb on - strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.833 kb on + strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.834 kb on - strandat 105.845 kb on + strandat 105.846 kb on - strandat 105.854 kb on + strandat 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.885 kb on + strand, within Pf1N1B4_100at 105.886 kb on - strand, within Pf1N1B4_100at 106.226 kb on + strand, within Pf1N1B4_101at 106.226 kb on + strand, within Pf1N1B4_101at 106.227 kb on - strand, within Pf1N1B4_101at 106.293 kb on + strand, within Pf1N1B4_101at 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.424 kb on + strandat 106.428 kb on - strandat 106.428 kb on - strandat 106.501 kb on + strandat 106.502 kb on - strandat 106.549 kb on + strandat 106.550 kb on - strandat 106.677 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Doxycycline hyclate 0.0001 mg/ml
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104,902 + Pf1N1B4_99 0.38 -1.2
104,902 + Pf1N1B4_99 0.38 +0.8
104,903 - Pf1N1B4_99 0.38 -1.9
104,903 - +2.7
105,020 - Pf1N1B4_99 0.49 +2.1
105,192 + Pf1N1B4_99 0.66 +0.0
105,192 + Pf1N1B4_99 0.66 -0.5
105,255 + Pf1N1B4_99 0.72 +0.2
105,268 + Pf1N1B4_99 0.73 -1.4
105,268 + Pf1N1B4_99 0.73 +0.2
105,269 - Pf1N1B4_99 0.73 -0.2
105,301 + Pf1N1B4_99 0.76 -1.9
105,301 + Pf1N1B4_99 0.76 +2.3
105,301 + Pf1N1B4_99 0.76 -1.9
105,301 + Pf1N1B4_99 0.76 -1.2
105,301 + Pf1N1B4_99 0.76 -0.5
105,302 - -1.2
105,302 - Pf1N1B4_99 0.76 -0.6
105,428 - -0.5
105,657 + +0.8
105,657 + -1.1
105,657 + -1.2
105,658 - +0.2
105,791 - +0.7
105,828 - -0.5
105,828 - +1.7
105,829 + -0.3
105,830 - -1.1
105,830 - +2.1
105,833 + -1.2
105,833 + -0.5
105,833 + -1.2
105,834 - -0.9
105,834 - +1.4
105,834 - +1.1
105,834 - -1.1
105,834 - +1.0
105,834 - +1.6
105,834 - +1.2
105,834 - -3.2
105,845 + -0.3
105,846 - -0.8
105,854 + +0.6
105,885 + Pf1N1B4_100 0.13 +1.5
105,885 + Pf1N1B4_100 0.13 -1.2
105,885 + Pf1N1B4_100 0.13 +1.1
105,886 - Pf1N1B4_100 0.14 +1.6
106,226 + Pf1N1B4_101 0.48 +1.5
106,226 + Pf1N1B4_101 0.48 +0.1
106,227 - Pf1N1B4_101 0.48 -0.1
106,293 + Pf1N1B4_101 0.88 +0.3
106,424 + +1.1
106,424 + -0.9
106,424 + -0.7
106,424 + +0.6
106,428 - +0.0
106,428 - +0.3
106,501 + +1.1
106,502 - -2.6
106,549 + -0.9
106,550 - -0.9
106,677 + +0.3

Or see this region's nucleotide sequence