Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS08085 and MMP_RS08090 are separated by 88 nucleotides MMP_RS08090 and MMP_RS08095 are separated by 11 nucleotides MMP_RS08095 and MMP_RS08100 are separated by 62 nucleotides
MMP_RS08085: MMP_RS08085 - hypothetical protein, at 1,522,102 to 1,523,037
_RS08085
MMP_RS08090: MMP_RS08090 - hypothetical protein, at 1,523,126 to 1,523,422
_RS08090
MMP_RS08095: MMP_RS08095 - dethiobiotin synthase, at 1,523,434 to 1,524,006
_RS08095
MMP_RS08100: MMP_RS08100 - 8-amino-7-oxononanoate synthase, at 1,524,069 to 1,525,181
_RS08100
Position (kb)
1523
1524 Strain fitness (log2 ratio)
-5
-4
-3
-2
-1
0
1 at 1522.265 kb on + strand, within MMP_RS08085 at 1522.309 kb on - strand, within MMP_RS08085 at 1522.349 kb on + strand, within MMP_RS08085 at 1522.377 kb on - strand, within MMP_RS08085 at 1522.387 kb on - strand, within MMP_RS08085 at 1522.414 kb on - strand, within MMP_RS08085 at 1522.458 kb on + strand, within MMP_RS08085 at 1522.479 kb on - strand, within MMP_RS08085 at 1522.480 kb on + strand, within MMP_RS08085 at 1522.570 kb on + strand, within MMP_RS08085 at 1522.595 kb on + strand, within MMP_RS08085 at 1522.658 kb on - strand, within MMP_RS08085 at 1522.664 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.672 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.704 kb on + strand, within MMP_RS08085 at 1522.707 kb on + strand, within MMP_RS08085 at 1522.727 kb on + strand, within MMP_RS08085 at 1522.847 kb on + strand, within MMP_RS08085 at 1522.859 kb on + strand, within MMP_RS08085 at 1522.951 kb on + strand at 1522.952 kb on - strand at 1522.991 kb on - strand at 1523.023 kb on - strand at 1523.024 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.029 kb on + strand at 1523.040 kb on - strand at 1523.064 kb on - strand at 1523.088 kb on + strand at 1523.091 kb on - strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.092 kb on + strand at 1523.093 kb on - strand at 1523.093 kb on - strand at 1523.094 kb on + strand at 1523.095 kb on - strand at 1523.095 kb on - strand at 1523.104 kb on + strand at 1523.104 kb on + strand at 1523.156 kb on - strand, within MMP_RS08090 at 1523.367 kb on - strand, within MMP_RS08090 at 1523.371 kb on + strand, within MMP_RS08090 at 1523.371 kb on + strand, within MMP_RS08090 at 1523.392 kb on + strand, within MMP_RS08090 at 1523.451 kb on - strand at 1523.575 kb on + strand, within MMP_RS08095 at 1523.707 kb on - strand, within MMP_RS08095 at 1523.758 kb on - strand, within MMP_RS08095 at 1523.762 kb on - strand, within MMP_RS08095 at 1523.763 kb on + strand, within MMP_RS08095 at 1523.801 kb on + strand, within MMP_RS08095 at 1523.815 kb on - strand, within MMP_RS08095 at 1523.920 kb on + strand, within MMP_RS08095 at 1523.924 kb on - strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.925 kb on + strand, within MMP_RS08095 at 1523.931 kb on - strand, within MMP_RS08095 at 1523.962 kb on - strand at 1523.965 kb on + strand at 1523.965 kb on + strand at 1523.965 kb on + strand at 1524.040 kb on + strand at 1524.058 kb on - strand at 1524.254 kb on - strand, within MMP_RS08100
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 1,522,265 + MMP_RS08085 0.17 +0.4 1,522,309 - MMP_RS08085 0.22 +0.1 1,522,349 + MMP_RS08085 0.26 +0.1 1,522,377 - MMP_RS08085 0.29 -0.6 1,522,387 - MMP_RS08085 0.30 +0.4 1,522,414 - MMP_RS08085 0.33 -0.0 1,522,458 + MMP_RS08085 0.38 +0.0 1,522,479 - MMP_RS08085 0.40 +0.6 1,522,480 + MMP_RS08085 0.40 +0.6 1,522,570 + MMP_RS08085 0.50 +0.2 1,522,595 + MMP_RS08085 0.53 -0.1 1,522,658 - MMP_RS08085 0.59 -0.2 1,522,664 + MMP_RS08085 0.60 +0.5 1,522,672 + MMP_RS08085 0.61 +0.5 1,522,672 + MMP_RS08085 0.61 +0.3 1,522,704 + MMP_RS08085 0.64 +0.2 1,522,704 + MMP_RS08085 0.64 +0.6 1,522,707 + MMP_RS08085 0.65 +0.7 1,522,727 + MMP_RS08085 0.67 +0.1 1,522,847 + MMP_RS08085 0.80 +0.5 1,522,859 + MMP_RS08085 0.81 +0.8 1,522,951 + +0.4 1,522,952 - +0.9 1,522,991 - +1.1 1,523,023 - -0.4 1,523,024 + +0.8 1,523,029 + +0.4 1,523,029 + -0.0 1,523,029 + -0.6 1,523,029 + +0.7 1,523,040 - +0.8 1,523,064 - +1.0 1,523,088 + +0.8 1,523,091 - -2.1 1,523,092 + +0.6 1,523,092 + +0.5 1,523,092 + +0.4 1,523,093 - +0.3 1,523,093 - +0.5 1,523,094 + +0.2 1,523,095 - +0.9 1,523,095 - +1.0 1,523,104 + -0.0 1,523,104 + +1.0 1,523,156 - MMP_RS08090 0.10 +0.6 1,523,367 - MMP_RS08090 0.81 +0.3 1,523,371 + MMP_RS08090 0.82 +0.1 1,523,371 + MMP_RS08090 0.82 +0.2 1,523,392 + MMP_RS08090 0.90 +0.4 1,523,451 - -0.4 1,523,575 + MMP_RS08095 0.25 -3.8 1,523,707 - MMP_RS08095 0.48 -4.0 1,523,758 - MMP_RS08095 0.57 -4.5 1,523,762 - MMP_RS08095 0.57 -5.0 1,523,763 + MMP_RS08095 0.57 -1.5 1,523,801 + MMP_RS08095 0.64 -3.3 1,523,815 - MMP_RS08095 0.66 -2.2 1,523,920 + MMP_RS08095 0.85 -3.3 1,523,924 - MMP_RS08095 0.86 -4.6 1,523,925 + MMP_RS08095 0.86 -3.6 1,523,925 + MMP_RS08095 0.86 -3.8 1,523,925 + MMP_RS08095 0.86 -4.3 1,523,931 - MMP_RS08095 0.87 -3.0 1,523,962 - -3.3 1,523,965 + -3.0 1,523,965 + -4.3 1,523,965 + -4.6 1,524,040 + -3.9 1,524,058 - -4.7 1,524,254 - MMP_RS08100 0.17 -5.4
Or see this region's nucleotide sequence