Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01710 and MMP_RS01715 are separated by 132 nucleotides MMP_RS01715 and MMP_RS01720 are separated by 0 nucleotides MMP_RS01720 and MMP_RS01725 are separated by 9 nucleotides
MMP_RS01710: MMP_RS01710 - shikimate kinase, at 316,643 to 317,494
_RS01710
MMP_RS01715: MMP_RS01715 - DUF447 family protein, at 317,627 to 318,172
_RS01715
MMP_RS01720: MMP_RS01720 - replication factor C large subunit, at 318,173 to 319,633
_RS01720
MMP_RS01725: MMP_RS01725 - class I SAM-dependent methyltransferase family protein, at 319,643 to 320,671
_RS01725
Position (kb) 318
319
320 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 317.543 kb on + strand at 317.549 kb on - strand at 317.558 kb on + strand at 317.559 kb on - strand at 317.570 kb on - strand at 317.570 kb on - strand at 317.573 kb on - strand at 317.573 kb on - strand at 317.576 kb on - strand at 317.578 kb on - strand at 317.579 kb on + strand at 317.581 kb on - strand at 317.581 kb on - strand at 317.587 kb on + strand at 317.587 kb on + strand at 317.614 kb on + strand at 317.626 kb on - strand at 317.627 kb on + strand at 317.718 kb on + strand at 317.718 kb on + strand at 317.762 kb on - strand at 317.770 kb on + strand at 317.773 kb on - strand at 317.774 kb on + strand at 317.786 kb on - strand at 317.865 kb on - strand at 318.064 kb on - strand at 318.065 kb on + strand at 318.096 kb on - strand at 318.100 kb on - strand at 318.268 kb on - strand at 318.525 kb on - strand, within MMP_RS01720 at 318.573 kb on - strand, within MMP_RS01720 at 319.396 kb on + strand, within MMP_RS01720 at 319.656 kb on + strand at 319.684 kb on + strand at 319.786 kb on - strand, within MMP_RS01725 at 319.831 kb on - strand, within MMP_RS01725 at 319.871 kb on + strand, within MMP_RS01725 at 319.985 kb on - strand, within MMP_RS01725 at 319.999 kb on + strand, within MMP_RS01725 at 320.173 kb on + strand, within MMP_RS01725 at 320.432 kb on + strand, within MMP_RS01725 at 320.584 kb on - strand at 320.594 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 317,543 + -3.6 317,549 - -2.5 317,558 + -2.5 317,559 - -1.2 317,570 - -0.7 317,570 - -0.1 317,573 - -0.3 317,573 - -0.4 317,576 - +0.3 317,578 - -0.3 317,579 + -0.1 317,581 - -1.0 317,581 - -0.4 317,587 + -1.5 317,587 + -1.3 317,614 + -2.5 317,626 - -3.6 317,627 + -5.1 317,718 + -3.5 317,718 + -2.1 317,762 - -5.3 317,770 + -3.1 317,773 - -2.8 317,774 + -5.4 317,786 - -2.2 317,865 - -2.9 318,064 - -2.8 318,065 + -3.3 318,096 - -2.7 318,100 - -3.5 318,268 - -2.4 318,525 - MMP_RS01720 0.24 -1.3 318,573 - MMP_RS01720 0.27 -3.9 319,396 + MMP_RS01720 0.84 +0.5 319,656 + -0.6 319,684 + -4.0 319,786 - MMP_RS01725 0.14 -2.4 319,831 - MMP_RS01725 0.18 -3.8 319,871 + MMP_RS01725 0.22 -5.6 319,985 - MMP_RS01725 0.33 -3.9 319,999 + MMP_RS01725 0.35 -1.9 320,173 + MMP_RS01725 0.52 -4.5 320,432 + MMP_RS01725 0.77 -3.2 320,584 - -4.0 320,594 + -4.3
Or see this region's nucleotide sequence