Experiment: H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01415 and MMP_RS08935 are separated by 331 nucleotides MMP_RS08935 and MMP_RS01420 are separated by 241 nucleotides MMP_RS01420 and MMP_RS01425 are separated by 229 nucleotides
MMP_RS01415: MMP_RS01415 - DUF2067 domain-containing protein, at 262,131 to 262,748
_RS01415
MMP_RS08935: MMP_RS08935 - hypothetical protein, at 263,080 to 263,229
_RS08935
MMP_RS01420: MMP_RS01420 - hypothetical protein, at 263,471 to 263,668
_RS01420
MMP_RS01425: MMP_RS01425 - tyrosine--tRNA ligase, at 263,898 to 264,827
_RS01425
Position (kb) 263
264 Strain fitness (log2 ratio) -5
-4
-3
-2
-1
0
1 at 262.625 kb on + strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.682 kb on - strand, within MMP_RS01415 at 262.729 kb on + strand at 262.734 kb on - strand at 262.748 kb on - strand at 262.762 kb on - strand at 262.789 kb on - strand at 262.790 kb on + strand at 262.793 kb on - strand at 262.796 kb on + strand at 262.824 kb on - strand at 262.825 kb on + strand at 262.825 kb on + strand at 262.828 kb on - strand at 262.828 kb on - strand at 262.834 kb on - strand at 262.835 kb on + strand at 262.839 kb on + strand at 262.849 kb on - strand at 262.926 kb on - strand at 262.927 kb on + strand at 263.020 kb on - strand at 263.020 kb on - strand at 263.069 kb on - strand at 263.072 kb on - strand at 263.427 kb on + strand at 263.454 kb on - strand at 263.476 kb on + strand at 263.486 kb on - strand at 263.618 kb on - strand, within MMP_RS01420 at 263.621 kb on + strand, within MMP_RS01420 at 263.681 kb on + strand at 263.706 kb on - strand at 263.732 kb on - strand at 263.736 kb on + strand at 263.758 kb on - strand at 263.778 kb on + strand at 263.801 kb on + strand at 263.838 kb on + strand at 263.838 kb on + strand at 263.902 kb on - strand at 263.909 kb on - strand at 264.233 kb on - strand at 264.238 kb on - strand at 264.238 kb on - strand at 264.291 kb on + strand at 264.341 kb on + strand at 264.368 kb on + strand at 264.512 kb on - strand at 264.547 kb on - strand at 264.547 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction H2 McCas, 2.5g/L bicarb, 200 mM MOPs, 40 psi H2CO2, SO4 FREE remove 262,625 + MMP_RS01415 0.80 -4.4 262,682 - MMP_RS01415 0.89 -1.2 262,682 - MMP_RS01415 0.89 -1.8 262,682 - MMP_RS01415 0.89 -2.9 262,729 + -3.1 262,734 - -2.3 262,748 - -2.2 262,762 - +0.4 262,789 - -1.8 262,790 + -4.5 262,793 - -5.7 262,796 + -3.0 262,824 - -1.8 262,825 + -2.7 262,825 + -4.9 262,828 - -3.3 262,828 - -3.4 262,834 - -2.3 262,835 + -0.8 262,839 + -0.2 262,849 - +0.2 262,926 - -0.1 262,927 + +0.9 263,020 - +1.2 263,020 - +0.2 263,069 - +0.1 263,072 - -0.4 263,427 + +0.4 263,454 - -0.2 263,476 + +0.2 263,486 - +0.1 263,618 - MMP_RS01420 0.74 +0.1 263,621 + MMP_RS01420 0.76 +0.5 263,681 + +0.5 263,706 - +0.4 263,732 - +0.1 263,736 + -0.4 263,758 - +0.3 263,778 + +0.1 263,801 + -2.5 263,838 + -3.3 263,838 + -2.9 263,902 - -4.0 263,909 - -2.7 264,233 - -2.0 264,238 - -2.8 264,238 - -2.2 264,291 + -1.7 264,341 + -2.8 264,368 + -2.8 264,512 - -2.3 264,547 - -2.4 264,547 - -3.5
Or see this region's nucleotide sequence