Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03276 and CCNA_R0075 are separated by 99 nucleotides CCNA_R0075 and CCNA_03277 are separated by 44 nucleotides
CCNA_03276: CCNA_03276 - glycerophosphoryl diester phosphodiesterase, at 3,442,782 to 3,443,771
_03276
CCNA_R0075: CCNA_R0075 - tRNA-Met, at 3,443,871 to 3,443,947
_R0075
CCNA_03277: CCNA_03277 - glycosyltransferase, at 3,443,992 to 3,445,041
_03277
Position (kb)
3443
3444 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4
5 at 3442.880 kb on + strand at 3442.887 kb on + strand, within CCNA_03276 at 3442.942 kb on - strand, within CCNA_03276 at 3442.947 kb on + strand, within CCNA_03276 at 3442.955 kb on - strand, within CCNA_03276 at 3443.000 kb on + strand, within CCNA_03276 at 3443.002 kb on - strand, within CCNA_03276 at 3443.008 kb on - strand, within CCNA_03276 at 3443.008 kb on - strand, within CCNA_03276 at 3443.023 kb on + strand, within CCNA_03276 at 3443.059 kb on + strand, within CCNA_03276 at 3443.114 kb on + strand, within CCNA_03276 at 3443.114 kb on + strand, within CCNA_03276 at 3443.122 kb on - strand, within CCNA_03276 at 3443.122 kb on - strand, within CCNA_03276 at 3443.166 kb on + strand, within CCNA_03276 at 3443.174 kb on - strand, within CCNA_03276 at 3443.179 kb on - strand, within CCNA_03276 at 3443.188 kb on + strand, within CCNA_03276 at 3443.193 kb on + strand, within CCNA_03276 at 3443.277 kb on - strand, within CCNA_03276 at 3443.279 kb on + strand, within CCNA_03276 at 3443.287 kb on - strand, within CCNA_03276 at 3443.287 kb on - strand, within CCNA_03276 at 3443.291 kb on + strand, within CCNA_03276 at 3443.299 kb on - strand, within CCNA_03276 at 3443.322 kb on - strand, within CCNA_03276 at 3443.340 kb on - strand, within CCNA_03276 at 3443.365 kb on + strand, within CCNA_03276 at 3443.399 kb on + strand, within CCNA_03276 at 3443.402 kb on - strand, within CCNA_03276 at 3443.407 kb on - strand, within CCNA_03276 at 3443.409 kb on + strand, within CCNA_03276 at 3443.450 kb on - strand, within CCNA_03276 at 3443.461 kb on - strand, within CCNA_03276 at 3443.461 kb on - strand, within CCNA_03276 at 3443.477 kb on - strand, within CCNA_03276 at 3443.478 kb on - strand, within CCNA_03276 at 3443.507 kb on + strand, within CCNA_03276 at 3443.527 kb on - strand, within CCNA_03276 at 3443.532 kb on + strand, within CCNA_03276 at 3443.535 kb on - strand, within CCNA_03276 at 3443.599 kb on + strand, within CCNA_03276 at 3443.599 kb on + strand, within CCNA_03276 at 3443.607 kb on - strand, within CCNA_03276 at 3443.622 kb on - strand, within CCNA_03276 at 3443.632 kb on + strand, within CCNA_03276 at 3443.632 kb on + strand, within CCNA_03276 at 3443.705 kb on - strand at 3443.712 kb on - strand at 3443.740 kb on - strand at 3443.756 kb on + strand at 3443.793 kb on + strand at 3443.802 kb on + strand at 3443.802 kb on + strand at 3443.810 kb on - strand at 3443.810 kb on - strand at 3443.810 kb on - strand at 3443.810 kb on - strand at 3443.810 kb on - strand at 3443.810 kb on - strand at 3443.894 kb on - strand, within CCNA_R0075 at 3443.901 kb on + strand, within CCNA_R0075 at 3443.915 kb on + strand, within CCNA_R0075 at 3443.922 kb on + strand, within CCNA_R0075 at 3443.953 kb on - strand at 3443.953 kb on - strand at 3443.953 kb on - strand at 3443.958 kb on - strand at 3443.990 kb on + strand at 3444.863 kb on - strand, within CCNA_03277
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 3,442,880 + -0.9 3,442,887 + CCNA_03276 0.11 +0.3 3,442,942 - CCNA_03276 0.16 -1.1 3,442,947 + CCNA_03276 0.17 -0.3 3,442,955 - CCNA_03276 0.17 -0.1 3,443,000 + CCNA_03276 0.22 -2.5 3,443,002 - CCNA_03276 0.22 +0.1 3,443,008 - CCNA_03276 0.23 +1.2 3,443,008 - CCNA_03276 0.23 -0.8 3,443,023 + CCNA_03276 0.24 +1.3 3,443,059 + CCNA_03276 0.28 -0.8 3,443,114 + CCNA_03276 0.34 -0.7 3,443,114 + CCNA_03276 0.34 -2.0 3,443,122 - CCNA_03276 0.34 -0.0 3,443,122 - CCNA_03276 0.34 +2.2 3,443,166 + CCNA_03276 0.39 -0.6 3,443,174 - CCNA_03276 0.40 +0.2 3,443,179 - CCNA_03276 0.40 -1.4 3,443,188 + CCNA_03276 0.41 +0.7 3,443,193 + CCNA_03276 0.42 -1.2 3,443,277 - CCNA_03276 0.50 +0.2 3,443,279 + CCNA_03276 0.50 -1.6 3,443,287 - CCNA_03276 0.51 -0.5 3,443,287 - CCNA_03276 0.51 -1.6 3,443,291 + CCNA_03276 0.51 -1.0 3,443,299 - CCNA_03276 0.52 +1.4 3,443,322 - CCNA_03276 0.55 +1.3 3,443,340 - CCNA_03276 0.56 +0.1 3,443,365 + CCNA_03276 0.59 +5.4 3,443,399 + CCNA_03276 0.62 +1.5 3,443,402 - CCNA_03276 0.63 -1.6 3,443,407 - CCNA_03276 0.63 +2.3 3,443,409 + CCNA_03276 0.63 +0.4 3,443,450 - CCNA_03276 0.67 -1.8 3,443,461 - CCNA_03276 0.69 +0.0 3,443,461 - CCNA_03276 0.69 -1.5 3,443,477 - CCNA_03276 0.70 -0.0 3,443,478 - CCNA_03276 0.70 +1.4 3,443,507 + CCNA_03276 0.73 +2.0 3,443,527 - CCNA_03276 0.75 +1.9 3,443,532 + CCNA_03276 0.76 -0.4 3,443,535 - CCNA_03276 0.76 +0.8 3,443,599 + CCNA_03276 0.83 -1.0 3,443,599 + CCNA_03276 0.83 -0.4 3,443,607 - CCNA_03276 0.83 +2.3 3,443,622 - CCNA_03276 0.85 +0.4 3,443,632 + CCNA_03276 0.86 -2.1 3,443,632 + CCNA_03276 0.86 -1.2 3,443,705 - +0.5 3,443,712 - +0.4 3,443,740 - +2.2 3,443,756 + -0.6 3,443,793 + -0.9 3,443,802 + -0.9 3,443,802 + -1.6 3,443,810 - +1.5 3,443,810 - +2.4 3,443,810 - +0.3 3,443,810 - -2.5 3,443,810 - +0.3 3,443,810 - -1.7 3,443,894 - CCNA_R0075 0.30 -2.5 3,443,901 + CCNA_R0075 0.39 +0.6 3,443,915 + CCNA_R0075 0.57 +0.6 3,443,922 + CCNA_R0075 0.66 -1.3 3,443,953 - +2.1 3,443,953 - +1.9 3,443,953 - +0.1 3,443,958 - +0.0 3,443,990 + -0.6 3,444,863 - CCNA_03277 0.83 +1.0
Or see this region's nucleotide sequence