Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_03562 and CCNA_03563 are separated by 52 nucleotides CCNA_03563 and CCNA_03564 are separated by 192 nucleotides CCNA_03564 and CCNA_03565 are separated by 153 nucleotides CCNA_03565 and CCNA_03993 are separated by 245 nucleotides
CCNA_03562: CCNA_03562 - ATP synthase alpha chain, at 3,717,106 to 3,718,638
_03562
CCNA_03563: CCNA_03563 - ATP synthase delta chain, at 3,718,691 to 3,719,245
_03563
CCNA_03564: CCNA_03564 - PaaI-family thioesterase-like protein, at 3,719,438 to 3,719,677
_03564
CCNA_03565: CCNA_03565 - hypothetical protein, at 3,719,831 to 3,720,154
_03565
CCNA_03993: CCNA_03993 - hypothetical protein, at 3,720,400 to 3,720,816
_03993
Position (kb)
3719
3720 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 3719.443 kb on - strand at 3719.451 kb on - strand at 3719.464 kb on - strand, within CCNA_03564 at 3719.488 kb on + strand, within CCNA_03564 at 3719.498 kb on + strand, within CCNA_03564 at 3719.498 kb on + strand, within CCNA_03564 at 3719.502 kb on + strand, within CCNA_03564 at 3719.503 kb on + strand, within CCNA_03564 at 3719.510 kb on - strand, within CCNA_03564 at 3719.510 kb on - strand, within CCNA_03564 at 3719.510 kb on - strand, within CCNA_03564 at 3719.512 kb on + strand, within CCNA_03564 at 3719.547 kb on - strand, within CCNA_03564 at 3719.552 kb on - strand, within CCNA_03564 at 3719.552 kb on - strand, within CCNA_03564 at 3719.624 kb on + strand, within CCNA_03564 at 3719.631 kb on + strand, within CCNA_03564 at 3719.631 kb on + strand, within CCNA_03564 at 3719.631 kb on + strand, within CCNA_03564 at 3719.639 kb on - strand, within CCNA_03564 at 3719.639 kb on - strand, within CCNA_03564 at 3719.666 kb on + strand at 3719.680 kb on + strand at 3719.737 kb on + strand at 3719.770 kb on - strand at 3719.771 kb on - strand at 3719.776 kb on - strand at 3719.800 kb on + strand at 3719.819 kb on - strand at 3719.819 kb on - strand at 3719.867 kb on + strand, within CCNA_03565 at 3719.867 kb on + strand, within CCNA_03565 at 3719.872 kb on + strand, within CCNA_03565 at 3719.875 kb on - strand, within CCNA_03565 at 3719.888 kb on - strand, within CCNA_03565 at 3719.927 kb on - strand, within CCNA_03565 at 3719.936 kb on - strand, within CCNA_03565 at 3719.941 kb on + strand, within CCNA_03565 at 3719.966 kb on - strand, within CCNA_03565 at 3719.975 kb on + strand, within CCNA_03565 at 3719.983 kb on - strand, within CCNA_03565 at 3720.008 kb on + strand, within CCNA_03565 at 3720.016 kb on - strand, within CCNA_03565 at 3720.016 kb on - strand, within CCNA_03565 at 3720.033 kb on + strand, within CCNA_03565 at 3720.033 kb on + strand, within CCNA_03565 at 3720.033 kb on + strand, within CCNA_03565 at 3720.033 kb on + strand, within CCNA_03565 at 3720.033 kb on + strand, within CCNA_03565 at 3720.041 kb on - strand, within CCNA_03565 at 3720.042 kb on - strand, within CCNA_03565 at 3720.042 kb on - strand, within CCNA_03565 at 3720.057 kb on - strand, within CCNA_03565 at 3720.062 kb on - strand, within CCNA_03565 at 3720.117 kb on - strand, within CCNA_03565 at 3720.121 kb on + strand, within CCNA_03565 at 3720.188 kb on - strand at 3720.198 kb on - strand at 3720.227 kb on + strand at 3720.377 kb on + strand at 3720.378 kb on + strand at 3720.398 kb on + strand at 3720.412 kb on + strand at 3720.413 kb on - strand at 3720.434 kb on + strand at 3720.442 kb on - strand, within CCNA_03993 at 3720.442 kb on - strand, within CCNA_03993 at 3720.454 kb on + strand, within CCNA_03993 at 3720.454 kb on + strand, within CCNA_03993 at 3720.462 kb on - strand, within CCNA_03993 at 3720.462 kb on - strand, within CCNA_03993 at 3720.467 kb on + strand, within CCNA_03993 at 3720.469 kb on - strand, within CCNA_03993 at 3720.516 kb on + strand, within CCNA_03993 at 3720.565 kb on + strand, within CCNA_03993 at 3720.565 kb on + strand, within CCNA_03993 at 3720.568 kb on - strand, within CCNA_03993 at 3720.568 kb on - strand, within CCNA_03993 at 3720.582 kb on - strand, within CCNA_03993 at 3720.597 kb on - strand, within CCNA_03993 at 3720.597 kb on - strand, within CCNA_03993 at 3720.597 kb on - strand, within CCNA_03993 at 3720.616 kb on - strand, within CCNA_03993 at 3720.616 kb on - strand, within CCNA_03993 at 3720.622 kb on + strand, within CCNA_03993 at 3720.622 kb on + strand, within CCNA_03993 at 3720.622 kb on + strand, within CCNA_03993 at 3720.622 kb on + strand, within CCNA_03993 at 3720.630 kb on - strand, within CCNA_03993 at 3720.630 kb on - strand, within CCNA_03993 at 3720.630 kb on - strand, within CCNA_03993 at 3720.630 kb on - strand, within CCNA_03993 at 3720.630 kb on - strand, within CCNA_03993
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 3,719,443 - -1.8 3,719,451 - -1.3 3,719,464 - CCNA_03564 0.11 +0.7 3,719,488 + CCNA_03564 0.21 +0.6 3,719,498 + CCNA_03564 0.25 +1.9 3,719,498 + CCNA_03564 0.25 -0.5 3,719,502 + CCNA_03564 0.27 +0.6 3,719,503 + CCNA_03564 0.27 +0.3 3,719,510 - CCNA_03564 0.30 +0.7 3,719,510 - CCNA_03564 0.30 +1.8 3,719,510 - CCNA_03564 0.30 -0.7 3,719,512 + CCNA_03564 0.31 +2.3 3,719,547 - CCNA_03564 0.45 +3.1 3,719,552 - CCNA_03564 0.47 +1.1 3,719,552 - CCNA_03564 0.47 -1.9 3,719,624 + CCNA_03564 0.78 +2.2 3,719,631 + CCNA_03564 0.80 -2.5 3,719,631 + CCNA_03564 0.80 +1.4 3,719,631 + CCNA_03564 0.80 +1.9 3,719,639 - CCNA_03564 0.84 +0.6 3,719,639 - CCNA_03564 0.84 -1.1 3,719,666 + +1.1 3,719,680 + -2.3 3,719,737 + +0.6 3,719,770 - -0.9 3,719,771 - +0.5 3,719,776 - +1.3 3,719,800 + +1.5 3,719,819 - +0.8 3,719,819 - +1.2 3,719,867 + CCNA_03565 0.11 +2.6 3,719,867 + CCNA_03565 0.11 +1.2 3,719,872 + CCNA_03565 0.13 +0.3 3,719,875 - CCNA_03565 0.14 -0.9 3,719,888 - CCNA_03565 0.18 -1.4 3,719,927 - CCNA_03565 0.30 -0.7 3,719,936 - CCNA_03565 0.32 +1.6 3,719,941 + CCNA_03565 0.34 -1.4 3,719,966 - CCNA_03565 0.42 -2.7 3,719,975 + CCNA_03565 0.44 +0.5 3,719,983 - CCNA_03565 0.47 -0.1 3,720,008 + CCNA_03565 0.55 +3.1 3,720,016 - CCNA_03565 0.57 +0.8 3,720,016 - CCNA_03565 0.57 -0.9 3,720,033 + CCNA_03565 0.62 -0.1 3,720,033 + CCNA_03565 0.62 +1.4 3,720,033 + CCNA_03565 0.62 +0.1 3,720,033 + CCNA_03565 0.62 +0.6 3,720,033 + CCNA_03565 0.62 -0.9 3,720,041 - CCNA_03565 0.65 +3.0 3,720,042 - CCNA_03565 0.65 -2.2 3,720,042 - CCNA_03565 0.65 -1.5 3,720,057 - CCNA_03565 0.70 +2.4 3,720,062 - CCNA_03565 0.71 -0.3 3,720,117 - CCNA_03565 0.88 -0.1 3,720,121 + CCNA_03565 0.90 -0.9 3,720,188 - +1.0 3,720,198 - -0.6 3,720,227 + -0.5 3,720,377 + +0.6 3,720,378 + -1.2 3,720,398 + -1.2 3,720,412 + +1.9 3,720,413 - +1.6 3,720,434 + -2.0 3,720,442 - CCNA_03993 0.10 +2.0 3,720,442 - CCNA_03993 0.10 -0.4 3,720,454 + CCNA_03993 0.13 -1.3 3,720,454 + CCNA_03993 0.13 -1.9 3,720,462 - CCNA_03993 0.15 -1.3 3,720,462 - CCNA_03993 0.15 -1.9 3,720,467 + CCNA_03993 0.16 +0.6 3,720,469 - CCNA_03993 0.17 -1.6 3,720,516 + CCNA_03993 0.28 -1.0 3,720,565 + CCNA_03993 0.40 +0.1 3,720,565 + CCNA_03993 0.40 -1.2 3,720,568 - CCNA_03993 0.40 +1.4 3,720,568 - CCNA_03993 0.40 +0.3 3,720,582 - CCNA_03993 0.44 -1.6 3,720,597 - CCNA_03993 0.47 +0.2 3,720,597 - CCNA_03993 0.47 -0.9 3,720,597 - CCNA_03993 0.47 -2.5 3,720,616 - CCNA_03993 0.52 -1.1 3,720,616 - CCNA_03993 0.52 -1.1 3,720,622 + CCNA_03993 0.53 -1.6 3,720,622 + CCNA_03993 0.53 -1.0 3,720,622 + CCNA_03993 0.53 +0.5 3,720,622 + CCNA_03993 0.53 -0.5 3,720,630 - CCNA_03993 0.55 +1.2 3,720,630 - CCNA_03993 0.55 +0.2 3,720,630 - CCNA_03993 0.55 +0.4 3,720,630 - CCNA_03993 0.55 +0.2 3,720,630 - CCNA_03993 0.55 +0.7
Or see this region's nucleotide sequence