Experiment: PYE with Nalidixic 0.06 mg/ml
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt CCNA_01718 and CCNA_01719 overlap by 4 nucleotides CCNA_01719 and CCNA_03893 are separated by 103 nucleotides CCNA_03893 and CCNA_01721 are separated by 22 nucleotides CCNA_01721 and CCNA_01722 overlap by 32 nucleotides CCNA_01722 and CCNA_01723 are separated by 92 nucleotides
CCNA_01718: CCNA_01718 - RNA polymerase ECF sigma factor, at 1,847,210 to 1,847,725
_01718
CCNA_01719: CCNA_01719 - hypothetical protein, at 1,847,722 to 1,848,030
_01719
CCNA_03893: CCNA_03893 - hypothetical protein, at 1,848,134 to 1,848,526
_03893
CCNA_01721: CCNA_01721 - RNA polymerase ECF sigma factor, at 1,848,549 to 1,849,133
_01721
CCNA_01722: CCNA_01722 - hypothetical protein, at 1,849,102 to 1,849,506
_01722
CCNA_01723: CCNA_01723 - 3-methyl-2-oxobutanoate hydroxymethyltransferase, at 1,849,599 to 1,850,426
_01723
Position (kb)
1848
1849
1850 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 1847.589 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand at 1847.601 kb on + strand, within CCNA_01718 at 1847.601 kb on + strand, within CCNA_01718 at 1847.606 kb on + strand, within CCNA_01718 at 1847.612 kb on + strand, within CCNA_01718 at 1847.614 kb on - strand, within CCNA_01718 at 1847.614 kb on - strand, within CCNA_01718 at 1847.616 kb on + strand, within CCNA_01718 at 1847.629 kb on + strand, within CCNA_01718 at 1847.629 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.651 kb on + strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.659 kb on - strand, within CCNA_01718 at 1847.705 kb on + strand at 1847.713 kb on - strand at 1847.739 kb on + strand at 1847.787 kb on + strand, within CCNA_01719 at 1847.858 kb on + strand at 1847.881 kb on + strand, within CCNA_01719 at 1848.025 kb on + strand at 1848.083 kb on + strand at 1848.083 kb on + strand at 1848.189 kb on - strand, within CCNA_03893 at 1848.268 kb on + strand, within CCNA_03893 at 1848.292 kb on + strand, within CCNA_03893 at 1848.323 kb on + strand, within CCNA_03893 at 1848.331 kb on - strand, within CCNA_03893 at 1848.386 kb on + strand, within CCNA_03893 at 1848.461 kb on + strand, within CCNA_03893 at 1848.495 kb on + strand at 1848.495 kb on + strand at 1848.520 kb on + strand at 1848.520 kb on + strand at 1848.536 kb on + strand at 1848.537 kb on + strand at 1848.589 kb on + strand at 1848.597 kb on - strand at 1848.614 kb on - strand, within CCNA_01721 at 1848.629 kb on + strand, within CCNA_01721 at 1848.666 kb on + strand, within CCNA_01721 at 1848.674 kb on - strand, within CCNA_01721 at 1848.675 kb on - strand, within CCNA_01721 at 1848.731 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.812 kb on + strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.820 kb on - strand, within CCNA_01721 at 1848.827 kb on + strand, within CCNA_01721 at 1848.881 kb on - strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.936 kb on + strand, within CCNA_01721 at 1848.988 kb on + strand, within CCNA_01721 at 1849.044 kb on + strand, within CCNA_01721 at 1849.052 kb on - strand, within CCNA_01721 at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.122 kb on + strand at 1849.130 kb on - strand at 1849.198 kb on + strand at 1849.200 kb on + strand, within CCNA_01722 at 1849.277 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.282 kb on + strand, within CCNA_01722 at 1849.520 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction PYE with Nalidixic 0.06 mg/ml remove 1,847,589 + CCNA_01718 0.73 -1.9 1,847,601 + +1.7 1,847,601 + CCNA_01718 0.76 +0.2 1,847,601 + CCNA_01718 0.76 +0.7 1,847,606 + CCNA_01718 0.77 -2.2 1,847,612 + CCNA_01718 0.78 +0.0 1,847,614 - CCNA_01718 0.78 -1.8 1,847,614 - CCNA_01718 0.78 -2.1 1,847,616 + CCNA_01718 0.79 +1.4 1,847,629 + CCNA_01718 0.81 -0.1 1,847,629 + CCNA_01718 0.81 -0.9 1,847,651 + CCNA_01718 0.85 +1.4 1,847,651 + CCNA_01718 0.85 -0.1 1,847,651 + CCNA_01718 0.85 +0.6 1,847,651 + CCNA_01718 0.85 +0.9 1,847,651 + CCNA_01718 0.85 +0.8 1,847,651 + CCNA_01718 0.85 +0.1 1,847,651 + CCNA_01718 0.85 -1.5 1,847,651 + CCNA_01718 0.85 -1.1 1,847,651 + CCNA_01718 0.85 +0.7 1,847,651 + CCNA_01718 0.85 -0.4 1,847,651 + CCNA_01718 0.85 -0.3 1,847,659 - CCNA_01718 0.87 +2.2 1,847,659 - CCNA_01718 0.87 -1.2 1,847,659 - CCNA_01718 0.87 +1.4 1,847,659 - CCNA_01718 0.87 -1.8 1,847,659 - CCNA_01718 0.87 -1.7 1,847,659 - CCNA_01718 0.87 +1.1 1,847,659 - CCNA_01718 0.87 +1.1 1,847,659 - CCNA_01718 0.87 -0.4 1,847,659 - CCNA_01718 0.87 +0.9 1,847,705 + -1.4 1,847,713 - -1.6 1,847,739 + -2.3 1,847,787 + CCNA_01719 0.21 +0.7 1,847,858 + +1.7 1,847,881 + CCNA_01719 0.51 -0.2 1,848,025 + -1.4 1,848,083 + -0.0 1,848,083 + +1.2 1,848,189 - CCNA_03893 0.14 +3.7 1,848,268 + CCNA_03893 0.34 +1.1 1,848,292 + CCNA_03893 0.40 +0.7 1,848,323 + CCNA_03893 0.48 +0.5 1,848,331 - CCNA_03893 0.50 +0.7 1,848,386 + CCNA_03893 0.64 +0.9 1,848,461 + CCNA_03893 0.83 -0.5 1,848,495 + +2.4 1,848,495 + +0.2 1,848,520 + +0.5 1,848,520 + +1.0 1,848,536 + -0.2 1,848,537 + -0.3 1,848,589 + +0.5 1,848,597 - +1.1 1,848,614 - CCNA_01721 0.11 +1.7 1,848,629 + CCNA_01721 0.14 -0.7 1,848,666 + CCNA_01721 0.20 -1.0 1,848,674 - CCNA_01721 0.21 -1.2 1,848,675 - CCNA_01721 0.22 +0.5 1,848,731 + CCNA_01721 0.31 -0.8 1,848,812 + CCNA_01721 0.45 +0.8 1,848,812 + CCNA_01721 0.45 +1.1 1,848,820 - CCNA_01721 0.46 +2.9 1,848,820 - CCNA_01721 0.46 +0.9 1,848,827 + CCNA_01721 0.48 +3.1 1,848,881 - CCNA_01721 0.57 -0.8 1,848,936 + CCNA_01721 0.66 +0.4 1,848,936 + CCNA_01721 0.66 +1.8 1,848,988 + CCNA_01721 0.75 +1.8 1,849,044 + CCNA_01721 0.85 +0.9 1,849,052 - CCNA_01721 0.86 +0.8 1,849,122 + +3.4 1,849,122 + -2.1 1,849,122 + -0.4 1,849,122 + -0.9 1,849,130 - +0.4 1,849,198 + +0.9 1,849,200 + CCNA_01722 0.24 -2.8 1,849,277 + CCNA_01722 0.43 -0.9 1,849,282 + CCNA_01722 0.44 -3.2 1,849,282 + CCNA_01722 0.44 -2.1 1,849,520 + -1.4
Or see this region's nucleotide sequence